Characterisation and quality assessment of binding sites on a propazine-imprinted polymer prepared by precipitation polymerisation

Cacho, Carmen; Turiel, Esther; Martín‐Esteban, Antonio; Pérez-Conde, C.; Cámara, Carmen

doi:10.1016/j.jchromb.2003.12.018

Cited by 88 publications

(58 citation statements)

References 14 publications

(21 reference statements)

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“…Antibodies can be difficult to generate against non-immunogenic molecule, they can be expensive and a reliance on animals in their production is viewed with some concern. As an alternative, this study uses molecular imprinting in order to develop an alternative analytical tool for use in the analysis of atrazine (Muldoon and Stanker, 1995;Bjarnason et al, 1999;Sergeyeva et al, 1999;Shoji et al, 2003;Matsui et al, 1997;Matsui et al, 2000) and pesticides in general (Surugiu et al, 2000(Surugiu et al, , 2001aHaupt, 2001;Pap et al, 2002;Cacho et al, 2004;Zhu et al, 2002;Bastide et al, 2005). These studies have shown that for atrazine-imprinted polymers the recognition process, and hence the selectivity of the polymer, relies on interactions between the polymer and the chlorine atom.…”

Section: Introductionmentioning

confidence: 99%

Concentration dependent atrazine–atrazine complex formation promotes selectivity in atrazine imprinted polymers

Lavignac

Brain

Allender

2006

Biosensors and Bioelectronics

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An atrazine (ATR) molecularly imprinted polymer (MIP) was prepared using a non-covalent strategy. The affinity and selectivity of the polymer was initially evaluated under non-equilibrium conditions and the polymer was shown to possess good template selectivity. The selectivity of the polymer was further investigated under equilibrium conditions and over a range of concentrations using Scatchard plots and Hill plots and by assessing distribution coefficients and normalised selectivity values. It was observed that both selectivity and affinity were dependent on the concentration of the ligand and that unusually selectivity and affinity were better at higher atrazine concentrations. It was concluded that this phenomenon resulted from the formation of atrazine-atrazine complexes during the pre-polymerisation stage and during rebinding and that the polymer demonstrated improved atrazine affinity when the conditions favoured complex formation.

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Section: Introductionmentioning

confidence: 99%

Concentration dependent atrazine–atrazine complex formation promotes selectivity in atrazine imprinted polymers

Lavignac

Brain

Allender

2006

Biosensors and Bioelectronics

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“…The template used in polymerization has a direct influence on the final morphology of the polymer obtained, as reported by Cacho et al 97 Fenuron imprinted polymer displayed discrete uniformly sized microspheres while propazine imprinted polymer consisted of agglomerates of different sizes. The same conclusion was obtained by Tamayo et al 98 when they prepared MIPs by PP for the extraction of phenylurea herbicides by using linuron or isoproturon as template.…”

Section: Precipitation Polymerizationmentioning

confidence: 72%

Recent advances in molecular imprinting technology: current status, challenges and highlighted applications

2011

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Molecular imprinting technology (MIT) concerns formation of selective sites in a polymer matrix with the memory of a template. Recently, molecularly imprinted polymers (MIPs) have aroused extensive attention and been widely applied in many fields, such as solid-phase extraction, chemical sensors and artificial antibodies owing to their desired selectivity, physical robustness, thermal stability, as well as low cost and easy preparation. With the rapid development of MIT as a research hotspot, it faces a number of challenges, involving biological macromolecule imprinting, heterogeneous binding sites, template leakage, incompatibility with aqueous media, low binding capacity and slow mass transfer, which restricts its applications in various aspects. This critical review briefly reviews the current status of MIT, particular emphasis on significant progresses of novel imprinting methods, some challenges and effective strategies for MIT, and highlighted applications of MIPs. Finally, some significant attempts in further developing MIT are also proposed (236 references).

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“…In addition, as stated below (see Table 1), the particles obtained have a more homogeneous binding site distribution compared to that exhibited in bulk polymers [23,37]. Although particles of approximately 1-µm diameter are typically obtained and are thus not really suitable for HPLC, the synthesis of 3-to 5-µm spherical particles has been recently reported [38] making them ideal for chromatographic purposes.…”

Section: Precipitation Polymerizationmentioning

confidence: 92%

Molecularly imprinted polymers: towards highly selective stationary phases in liquid chromatography and capillary electrophoresis

Turiel

Martín‐Esteban

2004

Analytical and Bioanalytical Chemistry

153

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Molecularly imprinted polymers (MIPs) are synthetic polymers with a predetermined selectivity for a given analyte, or group of structurally related compounds, that make them ideal materials to be used in separation processes. In this sense, it is not surprising that the first applications of MIPs were as tailor-made chiral stationary phases in liquid chromatography. However, peak broadening and tailing, especially of the more retained enantiomers, were observed. Accordingly, this paper gives an overview of the attempts carried out during the recent years to improve the chromatographic performance of MIPs in liquid chromatography and capillary electrophoresis as well as the more recent applications. We conclude that MIPs are very promising materials to be used as selective stationary phases in chromatography although further developments are necessary in order to fully exploit their potential.

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Characterisation and quality assessment of binding sites on a propazine-imprinted polymer prepared by precipitation polymerisation

Cited by 88 publications

References 14 publications

Concentration dependent atrazine–atrazine complex formation promotes selectivity in atrazine imprinted polymers

Concentration dependent atrazine–atrazine complex formation promotes selectivity in atrazine imprinted polymers

Recent advances in molecular imprinting technology: current status, challenges and highlighted applications

Molecularly imprinted polymers: towards highly selective stationary phases in liquid chromatography and capillary electrophoresis

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