Characterisation and stability of anthocyanins in purple-fleshed sweet potato P40

Abstract

11Purple-fleshed sweet potato P40 has been shown to prevent colorectal cancer in a murine 12 model. This study is to identify anthocyanins by using HPLC/MS-MS and assess the stability 13 during various cooking conditions. P40 possesses a high content of anthocyanins up to 14 mg/g 14 dry matter. Total 12 acylated anthocyanins are identified. Top three anthocyanins, e.g., cyanidin 15 3-caffeoyl-p-hydroxybenzoyl sophoroside-5-glucoside, peonidin 3-caffeoyl sophoroside-5-16 glucoside, and cyanidin 3-(6'' -caffeoyl-6''-feruloylsophoroside)-5-glucoside, account for half 17 of the anthocyanin contents. Over 80% of anthocyanins measured by acid hydrolysis were 18 cyanidin derivatives, indicating P40 is unique when compared with other purple-fleshed sweet 19 potatoes that usually contain more peonidin than cyanidin. Steaming, pressure cooking, 20 microwaving, and frying but not baking significantly reduced 8-16% of total anthocyanin 21 contents. Mono-acylated anthocyanins showed a higher resistance against heat than di-and non-22 acylated. Among of which, cyanidin 3-p-hydroxybenzoylsophoroside-5-glucoside exhibited the 23 best thermal stability. The stable acylated and cyanidin-predominated anthocyanins in P40 may 24 provide extra benefits for cancer prevention. 25Keywords: Anthocyanins / purple-fleshed sweet potato / cancer prevention / stability / cooking 26 conditions 27

Phenotypic Diversity of Anthocyanins in Sorghum Accessions with Various Pericarp Pigments

“…According to our previous publications [19,28], Shimadzu HPLC system (Kyoto, Japan) was used for chromatographic analysis and separation. This system employed a DGU-20A3 built in degasser, a LC-20AB solvent delivery pump, a SIL-20ACHT auto-sampler, a CTO-20AC column-holding oven, a CBM-20A communicator module, and a SPD-M20A Photodiode Array Detectors.…”

“…They are synthesized by the flavonoid branch of the phenylpropanoid pathway through secondary metabolism in the plants. Among the over 600 types of anthocyanins [17], the majority of anthocyanin aglycone found in the food items usually consists of six anthocyanidins, i.e., cyanidin, delphinidin, petunidin, peonidin, pelargonidin, and malvidin [18,19]. However, sorghum anthocyanins are unique 3-deoxyanthocyanidins, which include the orange luteolinidin and the yellow apigeninidin [20,21].…”

Anti-proliferative and Pro-apoptotic Effects by Lees Extracts of Ehwa Makgeolli Containing Oriental Herbs

“…최근 대장암을 비롯한 여 러 가지 암 예방을 위한 다양한 천연물 발굴 및 식품 개발을 위한 연구가 활발하게 진행되고 있다 [4,16]. 식물에서 분리한 생리활성 물질들이 항암 활성이 높은 것들이 많으며, 암세포 의 사멸을 유도하는 대표적인 물질로는 안토시아닌(anthocyanin) [19], 아세토제닌(acetogenin) [2] 그리고 탁솔(taxol) …”

Effect of heat treatment on forage quality of bio-fortified orange fleshed Ipomea batatas crop residues and roots

“…Sweet potato are an important food security crop especially in rural areas (Xu et al, 2015;Mohanraj and Sivasankar, 2014;Sun et al, 2014a). Ruminant livestock scavenge on crop residues.…”