Characterisation of a class 1 integron associated with the formation of quadruple blaGES-5 cassettes from an IncP-1β group plasmid in Pseudomonas aeruginosa

Xu, Teng; Wang, Jian; Ying, Jianchao; Zhu, Tingyuan; Liu, Yabo; Xu, Lei; Li, Pingping; Li, Peizhen; Ying, Jun; Li, Kewei; Yi, Huiguang; Lü, Jing; Hu, Yunliang; Zhou, Tieli; Bao, Qiyu

doi:10.1016/j.ijantimicag.2018.07.002

Cited by 21 publications

(13 citation statements)

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“…Several types of integron classes have been found nowadays in bacteria [3]. Integrons are often located on transmissible plasmids or transposons, which can facilitate the transferability of antimicrobial genes among bacteria [4].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Huang

Lan

et al. 2020

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Background. Escherichia coli sequence type 131 (ST131) is an important multidrug-resistant extraintestinal pathogen, which can cause many kinds of infections. Integrons may play a crucial role in the dissemination of antibiotic resistance genes. e purpose of this study was to characterize the prevelance of integrons among E. coli ST131 strains in China. Methods. Eighty-three E. coli ST131 isolates were used in this study. e antibiotic susceptibility test was performed by the disk diffusion method. e presence and characterization of class 1, 2, and 3 integrons, as well as promotor of gene cassettes and other antimicrobial resistance genes, were detected by PCR and DNA sequencing. Transfer of integrons was carried out using a broth culture mating method. Clonal relatedness of E. coli ST131 isolates was analyzed by PFGE. Results. Overall, 26.5% (22/83) of the E. coli ST131 isolates carried class 1 integrons. Class 2 and 3 integrons were not found in this study. Two types of gene cassette arrays were demonstrated in this study and were as follows: dfrA17-aadA5 and aac(6′)-Ib-cr-cmlA5. Only one type of Pc promoter variant was detected among 22 integron-positive isolates (PcW). In vivo transfer of integron was successful for 9 of integron-positive E. coli ST131 isolates harboring resistance gene cassettes. Results of PFGE demonstrated that the integron-positive E. coli ST131 isolates were grouped into 12 different PFGE clusters. Conclusions. Our study showed a low prevalence of integrons was detected in E. coli ST131. Continued surveillance of this mobile genetic element should be performed to study the evolution of antibiotic resistance among E. coli ST131.

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Section: Introductionmentioning

confidence: 99%

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Huang

Lan

et al. 2020

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…Most recently, a clinical P. aeruginosa isolate named P. aeruginosa PA1280 belonging to ST1119 with strong carbapenems resistance was characterized. The major mechanism of high-level carbapenems resistance in P. aeruginosa PA1280 is that it harboured an IncP-1β conjugative plasmid, termed pICP-4GES, where a class 1 integron containing four consecutive bla GES-5 gene cassettes was located 7. In addition to four copies of bla GES-5 , the plasmid pICP-4GES also harboured a putative Ambler class A β-lactamase gene predictively encoding 303 amino acids (accession number APC57487), which was embedded in a Tn 3 -like transposon 7.…”

Section: Introductionmentioning

confidence: 99%

<p>PAU-1, a Novel Plasmid-Encoded Ambler Class A β-Lactamase Identified in a Clinical <em>Pseudomonas aeruginosa</em> Isolate</p>

Jian

Ying

et al. 2019

IDR

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PurposeThe aim of this work was to identify a novel β-lactamase gene blaPAU-1 encoded on the plasmid of a clinical Pseudomonas aeruginosa isolate.Materials and methodsThe clinical P. aeruginosa isolates were isolated from a hospital in southern China. Molecular cloning was performed to analyze the function of the resistance gene. The minimum inhibitory concentration (MIC) was determined by means of the agar dilution method to determine the antimicrobial susceptibilities of the strains. Whole-genome sequencing and comparative genomics analysis were performed to analyze the structures of the resistance gene-related sequences.ResultsPAU-1 is a molecular class A, Bush-Jacoby group 2be enzyme which encoded 293 amino acids and shared 74% amino acid identity with a putative class A β-lactamase from Rhodoferax saidenbachensis. Cloned blaPAU-1 in Escherichia coli and P. aeruginosa conferred resistance to piperacillin and ampicillin, and elevated the MIC with a 2–3 dilution for some oxyimino-β-lactams in P. aeruginosa. The genetic environment of blaPAU-1 is tnpA-res-hp-relE-blaPAU-1-lysR, which is in accordance with the structure of a Tn3 transposon. Epidemiological investigation of blaPAU-1 in the same district did not show any evidences of molecular dissemination associated with this determinant.ConclusionA novel class A β-lactamase gene, blaPAU-1, associated with the mobile genetic element was identified on a transferable plasmid in a clinical P. aeruginosa isolate. Strict surveillance for the emergence of the new determinant should be established and an effort should be made to block the dissemination of this determinant.

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“…The bla KLUC-5 -positive strain and rifampin-resistant E . coli EC600 strain were used as the donor and recipient, respectively, to conduct conjugation assays as described elsewhere ( Xu et al, 2018 ). The transconjugant was selected on Mueller-Hinton agar plates containing 1800 μg/ml rifampin and 512 μg/ml ampicillin.…”

Section: Methodsmentioning

confidence: 99%

Characterization of a Novel blaKLUC Variant With Reduced β-Lactam Resistance From an IncA/C Group Plasmid in a Clinical Klebsiella pneumoniae Isolate

Shen

Zhang

et al. 2018

Front. Microbiol.

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Similar to other CTX-M family enzymes, KLUC is a recently identified and emerging determinant of cefotaxime resistance that has been recovered from at least three Enterobacteriaceae species, including Kluyvera cryocrescens, Escherichia coli, and Enterobacter cloacae. Whether this extended-spectrum β-lactamase (ESBL) has been disseminated among commonly isolated Enterobacteriaceae is worthy of further investigation. In this study, we screened 739 nosocomial Enterobacteriaceae isolates (240 Klebsiella pneumoniae and 499 E. coli strains) and found that one K. pneumoniae and four E. coli isolates harbored the blaKLUC gene. Three blaKLUC determinants isolated from E. coli were entirely identical to a blaKLUC-3 gene previously recovered in the same hospital. PFGE of four blaKLUC-harboring E. coli strains showed that prevalence of these determinants was most likely mediated by horizontal gene transfer but not clonal dissemination. However, the variant isolated from K. pneumoniae belonged to a novel member of the KLUC enzyme group. This newly identified enzyme (KLUC-5) has an amino acid substitution compared with previously identified KLUC-1 (G18S) and KLUC-3 (G240D). Antimicrobial susceptibility tests showed that KLUC-5 significantly reduced resistance activity to almost all the selected antimicrobials compared to previously identified KLUC-3. Site-directed mutagenesis showed that blaKLUC-5-D240G and blaKLUC-5-S18G significantly enhanced the MIC against its best substrate. Conjugation and S1-PFGE indicated that blaKLUC-5 was located on a transferable plasmid, which was further decoded by single-molecule, real-time sequencing. Comparative genome analysis showed that its backbone exhibited genetic homology to the IncA/C incompatibility group plasmids. A transposable element, ISEcp1, was detected 256-bp upstream of the blaKLUC-5 gene; this location was inconsistent with the previously identified blaKLUC-1 but congruent with the variants recovered from E. coli in the same hospital. These data provide evidence of the increasingly emerging KLUC group of ESBLs in China.

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Characterisation of a class 1 integron associated with the formation of quadruple blaGES-5 cassettes from an IncP-1β group plasmid in Pseudomonas aeruginosa

Cited by 21 publications

References 41 publications

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

<p>PAU-1, a Novel Plasmid-Encoded Ambler Class A β-Lactamase Identified in a Clinical <em>Pseudomonas aeruginosa</em> Isolate</p>

Characterization of a Novel blaKLUC Variant With Reduced β-Lactam Resistance From an IncA/C Group Plasmid in a Clinical Klebsiella pneumoniae Isolate

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