Characterisation of a novel GR31 plasmid co-harbouring blaNDM-1 and blaOXA-58 in an Acinetobacter sp. isolate

Genomic study of Acinetobacter baumannii strains co-harboring blaOXA-58 and blaNDM-1 reveals a large multidrug-resistant plasmid encoding these carbapenemases in Brazil

“…Interestingly, none of the STs described here belonged to the main clonal complexes CC1, CC15, CC25, and CC79 predominant in South America or to CC2, which is the most prevalent clonal lineage in the world ( Rodríguez et al, 2018 ). The same diversity of plasmids co-harboring bla OXA-58 and bla NDM-1 can also be highlighted, considering isolates from other countries ( Alattraqchi et al, 2021 ; Liu et al, 2021 ; Li et al, 2022a , b ).…”

“… Genomic comparison of plasmid pCCBH31258 with (A) other Acinetobacter baumannii strains from this study and (B) selected plasmids from GenBank: pC54_001 (CP042365.1), pDETAB2 (CP047975.1) ( Liu et al, 2021 ), pNDM_SCLZS86 (CP090865.1) ( Li et al, 2022b ), pNDM_SCLZS30 (CP090384.1) ( Li et al, 2022a ), pAC1530 (CP045561.1) ( Alattraqchi et al, 2021 ), and pAC1633-1 (CP059301) ( Alattraqchi et al, 2021 ). Inside out, the first ring is the plasmid pCCBH31258, used as a reference (CP101888.1).…”

“…Only the strains isolated in Malaysia and China have the bla OXA-58 and bla NDM-1 genes located on the same plasmid ( Alattraqchi et al, 2021 ; Liu et al, 2021 ) ( Figure 2B ), while in the Algerian isolate, the genes were chromosomally located ( Ramoul et al, 2016 ), and the genome of the Japanese strain is a draft ( Oinuma et al, 2016 ). Other non- baumannii Acinetobacter carrying bla OXA-58 and bla NDM-1 genes have been reported from Malaysia ( Alattraqchi et al, 2021 ), China ( Zhou et al, 2015 ; Chen et al, 2019 ; Li et al, 2022a , b ), and Palestine ( Regeen et al, 2014 ). Of these reports, three isolates were sequenced and found to carry these genes on the same plasmids ( Alattraqchi et al, 2021 ; Li et al, 2022a , b ) ( Figure 2B ).…”

“…Other non- baumannii Acinetobacter carrying bla OXA-58 and bla NDM-1 genes have been reported from Malaysia ( Alattraqchi et al, 2021 ), China ( Zhou et al, 2015 ; Chen et al, 2019 ; Li et al, 2022a , b ), and Palestine ( Regeen et al, 2014 ). Of these reports, three isolates were sequenced and found to carry these genes on the same plasmids ( Alattraqchi et al, 2021 ; Li et al, 2022a , b ) ( Figure 2B ). To the best of our knowledge, this is the first study reporting A. baumannii strains in Brazil co-harboring bla OXA-58 and bla NDM-1 genes.…”