Characterisation of a pathogenic virus isolated from marine threadfin fish (Eleutheronema tetradactylus) during a disease outbreak

Histopathological Changes Probably Associated with a Virus in Larval and Juvenile Marbled Sole Pleuronectes yokohamae

“…Complete virions had an elec tron-dense core surrounded by an double layered nucleocapsid with a hexagonal outline and an edge to-edge diameter of 75-80 nm. These morphological features of complete virions and the virion replicating process resemble those of viruses in the family Reoviridae (Rivas et al, 1998;Mertens et al, 2000;Nibert and Schiff, 2001;Seng et al, 2002). The forma tion of VAS was observed neither in slightly atrophic hepatocytes nor normal-looking hepatocytes.…”

An improved RT-PCR assay for rapid and sensitive detection of grass carp reovirus

“…The threadfin reovirus (TFV) was kindly provided by Dr. Yokemin Sin (National University of Singapore) (Seng et al, 2002), and other viruses isolated from cultured aquatic animals, such as chum salmon reovirus (CSV), white spot syndrome virus (WSSV), infectious pancreatic necrosis virus (IPNV), and Chinese mandarin fish iridovirus (CMIV), were obtained from the Wuhan Institute of Virology and Institute of Hydrobiology and used for this study.…”

Development of a rapid, sensitive and specific diagnostic assay for fish Aquareovirus based on RT-PCR

“…BF-2 cell line was used to propagate TFV as previously described by Seng et al (2002). Similarly, GPV was also propagated in BF-2.…”

“…Purified TFV, GPV and GCRV were obtained from infected cell culture supernatants by centrifugation and genomic dsRNA was extracted using the High Pure TM Viral RNA Kit (Boehringer Mannheim) as described by Seng et al (2002). The dsRNA was resuspended in DEPC-treated water and stored at −80 • C until use.…”

“…Recently, another pathogenic aquareovirus strain was isolated and reported by Chang et al (2002) from moribund threadfin fish. This virus was then characterized further by Seng et al (2002) and designated as Threadfin reovirus (TFV). The TFV virions are non-enveloped, icosahedral, 75-80 nm in diameter and consist of a double-layer capsid.…”