Characterisation of a resource population of pigs screened for resistance to salmonellosis

Diemen, Pauline M. van; Kreukniet, M.B.; Galina, Lucina; Bumstead, Nat; Wallis, Timothy S.

doi:10.1016/s0165-2427(02)00165-4

Cited by 37 publications

(45 citation statements)

References 22 publications

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“…These strains have been extensively characterized following oral inoculation of pigs (53) and infection of porcine alveolar macrophages (53) and in bovine oral challenge and ligated ileal loop models (34). Their behavior in such models is comparable 24,36,48, and 72 h after oral inoculation of pigs are shown. Triplicate samples were taken from each tissue, and means were calculated to give a value per animal.…”

Section: Discussionmentioning

confidence: 99%

“…These data are consistent with the known role of aromatic amino acid biosynthesis in Salmonella replication in vivo (33). Since the system had been validated, plasmid partitioning was used to measure the net growth of serovar Typhimurium 4/74 and serovar Choleraesuis A50 following oral inoculation of pigs by sacrificing groups of at least 3 pigs at intervals of 24,36,48, and 72 h postinfection. The data indicated that serovar Typhimurium 4/74 replicates faster in the ileal and colonic mucosa than does serovar Choleraesuis A50, whereas strain A50 reached higher numbers in draining mesenteric lymph nodes than did strain 4/74.…”

Section: Vol 75 2007 Net Replication Of S Enterica Serovars In Pigmentioning

confidence: 99%

“…Indeed, strains of serovars that are systemically avirulent in these hosts following oral inoculation, including serovar Typhimurium, exhibited increased invasion (9,34,47) and elicited greater enteropathogenic responses (34,47). Furthermore, although the natural resistance-associated macrophage protein is important in the control of systemic serovar Choleraesuis infection in pigs (48), strains of serovar Choleraesuis do not exhibit an ability to survive better in porcine primary alveolar macrophages compared to serovar Typhimurium and no significant differences in the induction of proinflammatory cytokines in cultured porcine alveolar macrophages by the serovars could be detected (53). It has been noted that host-restricted serovars persist better in mesenteric lymph nodes, indicating that bacterial net growth at intestinal sites may be an important determinant of systemic virulence (34,47).…”

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confidence: 99%

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Net Replication ofSalmonella entericaSerovars Typhimurium and Choleraesuis in Porcine Intestinal Mucosa and Nodes Is Associated with Their Differential Virulence

et al. 2007

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Salmonella enterica is a facultative intracellular pathogen of worldwide importance and causes a spectrum of diseases depending on serovar-and host-specific factors. Oral infection of pigs with S. enterica serovar Typhimurium strain 4/74 produces acute enteritis but is rarely fatal, whereas serovar Choleraesuis strain A50 causes systemic disease with a high mortality rate. With a porcine ligated ileal loop model, we observed that systemic virulence of serovar Choleraesuis A50 is not associated with enhanced intestinal invasion, secretory responses, or neutrophil recruitment compared to serovar Typhimurium 4/74. The net growth in vivo of serovar Choleraesuis A50 and serovar Typhimurium 4/74 was monitored following oral inoculation of pigs with strains harboring pHSG422, which exhibits temperature-sensitive replication. Analysis of plasmid partitioning revealed that the enteric virulence of serovar Typhimurium 4/74 relative to that of serovar Choleraesuis A50 is associated with rapid replication in the intestinal wall, whereas systemic virulence of serovar Choleraesuis A50 is associated with enhanced persistence in intestinal mesenteric lymph nodes. Faster replication of serovar Typhimurium, compared to that of serovar Choleraesuis, in the intestinal mucosa was associated with greater induction of the proinflammatory cytokines tumor necrosis factor alpha, interleukin-8 (IL-8), and IL-18 as detected by reverse transcriptase PCR analysis of transcripts from infected mucosa. During replication in batch culture and porcine alveolar macrophages, transcription of genes encoding components of type III secretion systems 1 (sipC) and 2 (sseC) was observed to be significantly higher in serovar Typhimurium 4/74 than in serovar Choleraesuis A50, and this may contribute to the differences in epithelial invasion and intracellular proliferation. The rapid induction of proinflammatory responses by strain 4/74 may explain why pigs confine serovar Typhimurium infection to the intestines, whereas slow replication of serovar Choleraesuis may enable it to evade host innate immunity and thus disseminate by stealth.

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Section: Discussionmentioning

confidence: 99%

Section: Vol 75 2007 Net Replication Of S Enterica Serovars In Pigmentioning

confidence: 99%

mentioning

confidence: 99%

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Net Replication ofSalmonella entericaSerovars Typhimurium and Choleraesuis in Porcine Intestinal Mucosa and Nodes Is Associated with Their Differential Virulence

et al. 2007

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“…Resistance to intestinal colonization of poultry by Salmonella is believed to be determined by multiple genes and is less well understood. Characterization of a resource population of pigs exhibiting heritable differences in resistance to systemic S. Choleraesuis infection revealed that resistant pigs had higher levels of circulating neutrophils with improved phagocytic and oxidative function, yet mitogen-induced proliferation of lymphocytes was impaired (van Diemen et al 2002). The underlying genetic basis of these effects is unknown.…”

Section: Impact Of Host Genetic Backgroundmentioning

confidence: 99%

Molecular insights into farm animal and zoonoticSalmonellainfections

Stevens

Humphrey

Maskell

2009

Phil. Trans. R. Soc. B

145

102

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Salmonella enterica is a facultative intracellular pathogen of worldwide importance. Infections may present in a variety of ways, from asymptomatic colonization to inflammatory diarrhoea or typhoid fever depending on serovar-and host-specific factors. Human diarrhoeal infections are frequently acquired via the food chain and farm environment by virtue of the ability of selected non-typhoidal serovars to colonize the intestines of food-producing animals and contaminate the avian reproductive tract and egg. Colonization of reservoir hosts often occurs in the absence of clinical symptoms; however, some S. enterica serovars threaten animal health owing to their ability to cause acute enteritis or translocate from the intestines to other organs causing fever, septicaemia and abortion. Despite the availability of complete genome sequences of isolates representing several serovars, the molecular mechanisms underlying Salmonella colonization, pathogenesis and transmission in reservoir hosts remain ill-defined. Here we review current knowledge of the bacterial factors influencing colonization of food-producing animals by Salmonella and the basis of host range, differential virulence and zoonotic potential.

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“…Breed differences and additive genetic variation in incidences of atrophic rhinitis, respiratory diseases, and enteric diseases have been reported (Lundeheim, 1979(Lundeheim, , 1988Van Diemen et al, 2002). Incidence of pigs in disease classifications and distributions of leukocytes in serum of pigs are low to moderately heritable (Henryon et al, 2001(Henryon et al, , 2002.…”

Section: Introductionmentioning

confidence: 99%

Differential immunity in pigs with high and low responses to porcine reproductive and respiratory syndrome virus infection1,2

Petry

Lunney

Boyd

et al. 2007

Journal of Animal Science

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ABSTRACT:One hundred Hampshire × Duroc crossbred pigs (HD) and 100 NE Index line (I) pigs were infected with porcine reproductive and respiratory syndrome (PRRS) virus and evaluated for resistance/susceptibility. Controls (100/line) were uninfected littermates to the infected pigs. Viremia, change in weight (WT⌬), and rectal temperature at 0, 4, 7, and 14 d postinfection were recorded. Lung, bronchial lymph node (BLN), and blood tissue were collected at necropsy (14 d postinfection). The first principal component from principal component analyses of all variables was used to rank the pigs for phenotypic response to PRRS virus. Low responders (low PRRS burden) had high WT⌬, low viremia, and few lung lesions; high responders (high PRRS burden) had low WT⌬, high viremia, and many lesions. The RNA was extracted from lung and BLN tissue of the 7 highest and 7 lowest responders per line

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Characterisation of a resource population of pigs screened for resistance to salmonellosis

Cited by 37 publications

References 22 publications

Net Replication ofSalmonella entericaSerovars Typhimurium and Choleraesuis in Porcine Intestinal Mucosa and Nodes Is Associated with Their Differential Virulence

Net Replication ofSalmonella entericaSerovars Typhimurium and Choleraesuis in Porcine Intestinal Mucosa and Nodes Is Associated with Their Differential Virulence

Molecular insights into farm animal and zoonoticSalmonellainfections

Differential immunity in pigs with high and low responses to porcine reproductive and respiratory syndrome virus infection1,2

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