Characterisation of a strain of Potato virus Y causing eggplant mosaic in southern Iran

Sadeghi, M. H.; Behjatnia, S. A. A.; Masumi, M.; Izadpanah, K.

doi:10.1071/ap07087

Cited by 19 publications

(5 citation statements)

References 16 publications

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“…Although PVY has long been known to occur in several regions of Iran [23,28,31,55,60], the coat protein (CP) and 3 0 -UTR nucleotide sequence of the eggplant [53] isolates of PVY from Iran has just recently been reported. To determine the diversity of PVY in Iran, we have analysed the biological, serological and molecular identity (P1, CP and 3 0 -UTR regions) of 14 PVY isolates.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of potato virus Y isolates from Iran

et al. 2010

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A survey of Potato virus Y (PVY) was conducted in cultivated fields in six Iranian provinces between January 2005 to July 2007. Two hundred samples from potato and tomato were collected and analyzed by enzyme-linked immunosorbent assay (ELISA) for potyviruses. Almost one fourth of the samples were found to be infected by PVY. Analysis of these PVY-positive samples using three monoclonal antibodies (MAbs) facilitating the simultaneous detection of three main strains namely the ordinary (PVY(O)), strain (PVY(N)) and C (PVY(C)) strains. However, the fourth strain (PVY(NTN)) and some others recombinant isolates were also identified by molecular methods. Host range and symptoms analysis using sap inoculation of four different strains of PVY onto a range of plants revealed that the four strains showed biological properties that seemed to be consistent with their molecular grouping. Fourteen isolates of PVY were chosen based on the host and geographical location, primer specificity and serology for further biological and molecular characterisation. The coat protein (CP) and P1 genes and 3'-non-translated region (3'NTR) from 14 representative isolates were sequenced and analysed with the sequences available in GenBank. Composite analysis of the P1, CP and 3'-UTR sequences with all full genome sequences of PVY revealed that there are three potential strains of PVY in Iran, PVY(O), PVY(N)-W and PVY(NTN). Isolate KER.SA(N) was the most divergent of all the 14 isolates reacted with PVY(N) specific MAbs but grouped with PVY(O) strains in maximum likelihood phylogentic analysis. The PVY(NTN) isolates from Iran more closely related to the European than North American PVY(NTN) isolates.

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Section: Introductionmentioning

confidence: 99%

Characterisation of potato virus Y isolates from Iran

et al. 2010

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“…Table 1 summarizes information about all viruses naturally infecting the eggplant plant. Some of these viruses, such as the cucumber mosaic virus (CMV, [17]), tomato spotted wilt orthotospovirus (TSWV, [26]) and eggplant mottled dwarf alphanucleorhabdovirus (EMDV, [27]) are polyphagous and have worldwide diffusion, while for others, e.g., potato virus Y (PVY, [14]) and pepino mosaic virus (PepMV, [22]) the host range is restricted to solanaceous species. Several studies have described the occurrence of Potato virus X in eggplant.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and Distribution of Major Viruses Infecting Eggplant in Lebanon and Molecular Characterization of a Local Potato Virus X Isolate

et al. 2021

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This research was carried out in order to evaluate the presence and distribution of viral infections causing severe disease in eggplant plants collected from different districts in Bekaa valley, Lebanon. Most infected plants showed virus-like symptoms consisting predominantly of leaf blotch, mottling chlorotic and ring spots; leaf twisting and plant dwarf were also observed in the visited fields. Symptomatic and asymptomatic plants were collected and screened by ELISA test for the presence of several different pathogenic viruses potentially present in the area. Results showed that potato virus Y (PVY) was the most prevalent virus found by ELISA (detected in the 15.3% of the tested plants), followed by eggplant mottled dwarf virus (EMDV, 2.9%) and cucumber mosaic virus (CMV, 1.2%), while tomato spotted wilt virus (TSWV), alfalfa mosaic virus (AMV) and pepper mottle virus (PepMoV) were not detected. Biological indexing of symptomatic ELISA-negative plants, followed by electron microscopy, indicated the presence of virus-like particles of the genus Potexvirus, which was subsequently confirmed as potato virus X (PVX) by RT-PCR and Sanger sequencing. PVX was found in 35.3% of the tested plants, all sampled in the northern Bekaa area. In a phylogenetic analysis, the partial coat protein gene sequence of a selected Lebanese isolate, PVX-AK1, clustered together with other PVX isolates from Asia. Furthermore, the 124-aa sequence of PVX-AK1 shared 100% identity with PVX-UK3, an isolate which is known as avirulent in potato genotypes carrying either Nx or Rx resistance genes. This work revealed a picture of the previously uninvestigated phytosanitary status of eggplant crops in an important horticultural area of Lebanon.

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“…Many viruses belonging to Tymovirus, Potyvirus, Cucumovirus, Tobamovirus and Carlavirus genera were reported to infect eggplant (Sastry, 1982;Vyanjane and Mali, 1984;Zhou et al, 2015;Hančinský et al, 2020), which can cause significant damage due to plant stunting, crinkling, and mottling accompanied by leaf and fruit abnormalities. The viruses that commonly occur in eggplant either in field or greenhouse cultivation are cucumber mosaic virus, CMV (Crescenzi et al, 1994;Bagewadi et al, 2015), potato virus Y, PVY (Sastry et al, 1974;Sadeghi et al, 2008), as well as alfalfa mosaic virus, AMV (Kemp and Troup, 1977;Al-Ani et al, 2011). Although AMV has been described as being able to infect eggplant, it is considered to be of minor economic importance to eggplant (Marchoux and Rougier, 1974;Al-Shahwan and Abdulla, 1998).…”

Section: Introductionmentioning

confidence: 99%

Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia

Milošević¹,

Ignjatov²,

Nikolić³

et al. 2021

Acta agriculturae Serbica

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In the 2018-19 growing season, a total of 51 leaves of eggplant plants grown under field conditions were collected randomly from nine private gardens at four different localities in the Province of Vojvodina. Eggplants with nearly 40% of plants showing bright yellow to white mosaic or mottling of leaves were found throughout the inspected fields (gardens). The collected samples were analyzed for the presence of alfalfa mosaic virus (AMV), cucumber mosaic virus (CMV) and potato virus Y (PVY) using commercial double-antibody sandwich (DAS)-ELISA kits. Serological analysis of eggplant samples revealed the presence of AMV in 80.39% collected samples. None of the analyzed samples was positive for CMV and PVY. The virus was successfully mechanically transmitted to test plants including Nicotiana benthamiana, Chenopodium quinoa, C. amaranticolor, as well as eggplant seedlings, confirming the infectious nature of the disease. The presence of AMV in eggplants was further verified by reverse transcription-polymerase chain reaction (RT-PCR) and sequencing, using the primers CP AMV1 and CP AMV2 that amplify part of the coat protein (CP) gene. The phylogenetic analysis showed that Serbian AMV isolates grouped into a separate well-supported group together with AMV isolates from Italy, Croatia and previously characterized isolates from Serbia. To our knowledge, this is the first report of AMV infection of eggplant in Serbia.

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Characterisation of a strain of Potato virus Y causing eggplant mosaic in southern Iran

Cited by 19 publications

References 16 publications

Characterisation of potato virus Y isolates from Iran

Characterisation of potato virus Y isolates from Iran

Occurrence and Distribution of Major Viruses Infecting Eggplant in Lebanon and Molecular Characterization of a Local Potato Virus X Isolate

Occurrence and molecular characterization of alfalfa mosaic virus in eggplant in Serbia

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