Characterisation of algicidal bacterial exometabolites against the lipid-accumulating diatom Skeletonema sp.

Wang, Hui; Butt, Lisa; Rooks, Paul; Khan, F. Nawaz; Allen, Michael J.; Ali, S. Twareque

doi:10.1016/j.algal.2015.11.012

Cited by 17 publications

(24 citation statements)

References 23 publications

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“…They are able to outgrow native bacterial communities in coastal areas ( 63 ) and degrade a variety of algal-derived molecules such as silica ( 64 ), nutrients released during decaying phytoplankton blooms ( 65 ), and a variety of diatom-derived polysaccharides ( 66 , 67 ). More specifically, A. macleodii competes with diatoms for nitrate ( 68 ), while closely related Alteromonas species contribute to the lysis of dinoflagellates ( 69 ) and produce an array of algicidal compounds effective against diatoms and other phytoplankton lineages ( 70 – 72 ). The placement of A. macleodii F12 within the A .…”

Section: Discussionmentioning

confidence: 99%

Diatom modulation of select bacteria through use of two unique secondary metabolites

Shibl

Isaac

Ochsenkühn

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

148

112

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Unicellular eukaryotic phytoplankton, such as diatoms, rely on microbial communities for survival despite lacking specialized compartments to house microbiomes (e.g., animal gut). Microbial communities have been widely shown to benefit from diatom excretions that accumulate within the microenvironment surrounding phytoplankton cells, known as the phycosphere. However, mechanisms that enable diatoms and other unicellular eukaryotes to nurture specific microbiomes by fostering beneficial bacteria and repelling harmful ones are mostly unknown. We hypothesized that diatom exudates may tune microbial communities and employed an integrated multiomics approach using the ubiquitous diatom Asterionellopsis glacialis to reveal how it modulates its naturally associated bacteria. We show that A. glacialis reprograms its transcriptional and metabolic profiles in response to bacteria to secrete a suite of central metabolites and two unusual secondary metabolites, rosmarinic acid and azelaic acid. While central metabolites are utilized by potential bacterial symbionts and opportunists alike, rosmarinic acid promotes attachment of beneficial bacteria to the diatom and simultaneously suppresses the attachment of opportunists. Similarly, azelaic acid enhances growth of beneficial bacteria while simultaneously inhibiting growth of opportunistic ones. We further show that the bacterial response to azelaic acid is numerically rare but globally distributed in the world’s oceans and taxonomically restricted to a handful of bacterial genera. Our results demonstrate the innate ability of an important unicellular eukaryotic group to modulate select bacteria in their microbial consortia, similar to higher eukaryotes, using unique secondary metabolites that regulate bacterial growth and behavior inversely across different bacterial populations.

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Section: Discussionmentioning

confidence: 99%

Diatom modulation of select bacteria through use of two unique secondary metabolites

Shibl

Isaac

Ochsenkühn

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

148

112

View full text Add to dashboard Cite

show abstract

“…Furthermore, this study demonstrates for the first time the antibacterial activity of bacterial strains affiliated to the genus Maribacter. Algicidal activity of Maribacter isolates has been reported (Wang et al 2016), but bioactivity of this genus of the Flavobacteriaceae family is virtually unexplored.…”

Section: Discussionmentioning

confidence: 99%

“…Algicidal activity of Maribacter isolates has been reported (Wang et al . 2016), but bioactivity of this genus of the Flavobacteriaceae family is virtually unexplored.…”

Section: Discussionmentioning

confidence: 99%

The sponges Hymeniacidon perlevis and Halichondria panicea are reservoirs of antibiotic‐producing bacteria against multi‐drug resistant Staphylococcus aureus

et al. 2021

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Aims: Evaluation of the antibacterial activity of cultivable bacteria associated with the marine sponges Hymeniacidon perlevis and Halichondria panicea against multi-drug-resistant Staphylococcus aureus. Methods and Results: One hundred and fourteen bacterial isolates were recovered from H. perlevis and H. panicea. Antibacterial action was demonstrated by 70% of the isolates against reference strain Staphylococcus aureus ATCC 29213 and by 31Á6% against Pseudomonas aeruginosa ATCC 27853 in agar overlay assays. Antibacterial potential was further analysed against 36 multi-drug-resistant hospital Staphylococcus aureus strains with diverse resistance profiles. Among the 80 isolates positive against S. aureus ATCC 29213, 76Á3% were active against at least one clinical S. aureus pathogen and 73Á6% inhibited one or more methicillin-resistant (MRSA) and vancomycin non-susceptible S. aureus strains. In addition, 41Á3% inhibited all vancomycin nonsusceptible MRSA strains. Conclusions: Culturable bacteria associated to H. perlevis and H. panicea are promising sources of antibacterial compounds of great pharmaceutical interest. Significance and Impact of the Study: This study was the first to explore the antibacterial potential of culturable bacteria associated with the marine sponges H. perlevis and H. panicea against MDR bacteria. This is the first report of antibacterial activity by Aquimarina, Denitrobaculum, Maribacter and Vagococcus isolates against MDR S. aureus strains, including vancomycin nonsusceptible and methicillin-resistant ones, against which new antibiotics are urgently needed.

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“…Structural analysis of algicidal substances has been a hot but difficult area in recent years (Talaat, 2019). A few algicidal substances have been isolated, mainly including macromolecules such as peptides, chitinase (Wang et al, 2016) luciferin (Zhang et al, 2016b), triterpene saponins (Luo et al, 2013), rhamnolipids (Xu et al, 2020), amino acids (Quan et al, 2021) and tryptamine (Zhang et al, 2016a).…”

Section: Algicidal Substancesmentioning

confidence: 99%

Algicidal substances of Brevibacillus laterosporus and their effect on red tide organisms

Liu

et al. 2023

Front. Mar. Sci.

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Herein, the algicidal effects of Brevibacillus laterosporus on typical red tide organisms were investigated. Through comparative analysis of the fermentation solution, sterile filtrate and bacterial body, it was found that this strain mainly exerts its algicidal function by secreting algicidal substances. In this paper, we established a method for extracting the algicidal substances of B. laterosporus and systematically investigated their features and effect on Heterosigma akashiwo. The results showed that the algicidal substances are a mixture of compounds all with molecular weights less than 3500 Da, with those below 100 Da producing 45% of the algicidal effect; these are more polar and best extracted with methanol. The algicidal substances are stable over a range of temperatures (-80.0 to 70.0°C) and pH values (4.0-8.0). The algicidal substances caused most H. akashiwo cells to rupture within 24 h. In the remaining cells, the algicidal substances activated the antioxidant system and reduced their metabolic activity, leading to apoptosis, as observed by cell crumbling and a reduction in membrane potential. The responses of different algal cell surface structures to the algicidal substances were also compared and analysed. It was concluded that these algicidal substances can act on the cell membrane and change its permeability, allowing entry of the algicidal substances to produce an algicidal effect.

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Characterisation of algicidal bacterial exometabolites against the lipid-accumulating diatom Skeletonema sp.

Cited by 17 publications

References 23 publications

Diatom modulation of select bacteria through use of two unique secondary metabolites

Diatom modulation of select bacteria through use of two unique secondary metabolites

The sponges Hymeniacidon perlevis and Halichondria panicea are reservoirs of antibiotic‐producing bacteria against multi‐drug resistant Staphylococcus aureus

Algicidal substances of Brevibacillus laterosporus and their effect on red tide organisms

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