Characterisation of an Aromatic Plant-based Formula using UV-Vis Spectroscopy, LC–ESI(+)QTOF-MS and HPLC-DAD Analysis

Abstract

Abstract. It is known for a long time that seasoning/condimentary herbs have antioxidant activity and antibacterial properties, being good natural alternatives for disease prevention. The different efficiency of these plants is assigned to their bioactive molecules, stability and bioavailability. In the present study seven aromatic herbs (basil, thyme, oregano, rosemary, clove, cinnamon and sage) were investigated individually. A new product was developed using basil, thyme, oregano, rosemary, clove, cinnamon and sage, according to a default recipe. The characterization of each plant aimed to identify the specific “fingerprint” by its main bioactive molecules and the “traceability” of these molecules in the new product, made by mixing the selected plants according to a default recipe. In order to determine the main bioactive compounds of the individual plants composition, in comparison with the new plant-based (EPC) formula, high throughput techniques like UV-Vis spectroscopy and LC-QTOF-MS spectrometry were used. The most important bioactive compounds determined in the studied herbs, which may exert antioxidant activity and antibacterial properties, were phenolic compounds (phenolic acids, flavonoids), quinones, clorophylls as well some polar terpenoids. The fingerprints are providing comprehensive and accurate information about the compounds that may exert antimicrobial properties. In order to assure the biological effects and the bioavailability of the polyphenols and the secondary metabolites we have to consider the antagonistic and synergistic effect that the metabolites can exert on each other.

Spectroscopic Studies on Pouzolzia Wightii Benn

“…The occurrence of UV-Vis spectroscopic around 280-330 nm indicates the existence of phenolic derivatives, at 330 nm confirms the presence of flavonoids in the crude extracts [20,21]. In the present study, the chlorophyll occurrence was confirmed in all the studied extracts of P. wightii.…”

Innovative phytosynthesized silver nanoarchitectures with enhanced antifungal and antioxidant properties

“…1 shows the UV-Vis spectra obtained for the diluted extracts -dilution factor 10 and, respectively, 100) performed on the extracts identified the maxima wavelengths specific to phenolic acids at 220-280 nm, to flavonoids at 290-420 nm and chlorophylls at 600-670 nm [23]. Table 1 represents the specific absorption values for the plant extracts, as well the extraction efficiency (EF factors) calculated according to the formula described in Section 2.…”

“…The obtained results were processed using specific data analysis software (Origin Pro 8.0). The Extraction Factor (EF) was determined, considering the absorption values (A max ), multiplied with the dilution factor (DF) [23].…”

Phytochemical and Antimicrobial Studies of Colloidal Silver Nanoparticles Mediated by <i>Laportea aestuans</i> Extract

“…Normalized UV-Vis spectra of L. aestuans extract and biosynthesized silver nanoparticles (L-Ag) in water are presented in Figure 3. Absorption of spectrum of L. aestuans extract revealed bands at around 289 and 320 nm, corresponding to the magnitude of resonance delocalization (( → ( conjugative characters) in the biomolecules in the plant extract [27]. Previous report has shown that absorption band around 280 and 330 nm could be due to the presence of polyphenols (tannins) and flavonoids, respectively [27].…”