Characterisation of Arthrobacter sp. S1 that can degrade α and β-haloalkanoic acids isolated from contaminated soil

Bagherbaigi, Saeedeh; Gicana, Ronnie G.; Lamis, Robert J.; Nemati, Mahdieh; Huyop, Fahrul

doi:10.1007/s13213-012-0595-4

Cited by 18 publications

(13 citation statements)

References 31 publications

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“…Successful amplification of the putative dehalogenase gene was obtained using primers from group I suggesting the dehalogenase from MF2 might fall in the same category. In contrast, Bagherbaigi et al [36] reported that strain S1 grew well on α/β-haloalkanoic acids (α/β-HA) and only group II primers [22] from Xanthobacter autotrophicus -dhlB314 and dhlB637 showed amplification. This observation was possibly due to L-2-haloacid dehalogenases being more common than group I dehalogenases.…”

Section: Sequencing and Analysis Of Putative Dehalogenase Genementioning

confidence: 92%

Further Analysis of <i>Burkholderia pseudomallei</i> MF2 and Identification of Putative Dehalogenase Gene by PCR

Edbeib¹,

Wahab

Huyop

et al. 2020

Indones. J. Chem.

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Halogenated organic compounds are extensively and widely used as pesticides, herbicides, and antibiotics that contribute to the pollution. This research was aimed to further analyze and characterize a bacterium that has the ability to utilize 2,2dichloropropionic acid (2,2-DCP) as a model to study dehalogenase enzyme production. Microscopic observation, biochemical tests and PCR technique were carried out in order to characterize the isolated bacterium. Strain MF2 showed its ability to grow on 10 mM 2,2-DCP liquid minimal medium with doubling time of 13 h with maximum chloride ion released of 19.8 molCl -/mL. The 16S rDNA analysis suggested that strain MF2 belongs to the genus Burkholderia. This was supported by the microscopic observation and biochemical tests. Dehalogenase gene was observed when using only primers dehIfor1 and dehIrev2 derived from group I deh PCR primer sequences, whereas no amplification using dhlB-314-forward and dhlB-637-reverse (group II dehalogenase) and haloacetate dehalogenase (H2-1157-forward and H2-1662-reverse) PCR primer sequences. The results suggested that, possibly, dehalogenase from MF2 was related to group I deh. In conclusion, strain MF2 showed the ability to utilize 2,2-DCP as sole source of carbon and energy. Further analysis revealed the MF2 strain consisted of dehalogenase gene that could be used for degradation of man-made halogenated compounds present in the environment. Using existing dehalogenase PCR primers, it was possible to amplify the dehalogenase genes sequence.

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Section: Sequencing and Analysis Of Putative Dehalogenase Genementioning

confidence: 92%

Further Analysis of <i>Burkholderia pseudomallei</i> MF2 and Identification of Putative Dehalogenase Gene by PCR

Edbeib¹,

Wahab

Huyop

et al. 2020

Indones. J. Chem.

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“…Interestingly, the Arthrobacter sp. S1 that could utilize a myriad of a-halo compounds such as a-halocarboxylic acid (aHA), 2,2-dichloropropionic acid (2,2-DCP) and D,L-2-chloropropionic acid (D,L-2-CP) as well as b-halocarboxylix acid (bHA) and 3-chloropropionic acid (3CP) as sole carbon source, exhibited cell doubling times of 5 ± 0.2 hours, 7 ± 0.1 hours and 10 ± 0.1 hours, respectively (Bagherbaigi et al, 2013). The variety of observed microbial growth speeds in media supplemented with 3CP may correlate with the well-known toxicity of this halogenated compound (Jing and Huyop, 2007).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a biodegrading 3-chloropropionic acid Burkholderia cepacia WH1 isolated from abandoned agricultural land

Muslem

Huyop

Zakaria

et al. 2015

Asia-Pacific Journal of Molecular Biology and Biotechnology

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The widespread use of herbicides that may contain the recalcitrant halogenated compound 3-chloropropionic acid poses significant environmental hazards and may prove detrimental to human kind. Therefore, it is important that an environmentally friendly bio-based method to detoxify such substance is developed. Consequently, the research detailed here investigated the isolation and identification of a bacterial strain that could degrade 3-chloropropionic acid (3CP) as its sole carbon source. A dehalogenase producing bacteria capable of utilizing 3CP was successfully isolated from abandoned agricultural land and designated strain WH1. Analysis of 16s rRNA as well as biochemical and morphological tests found that the strain WH1 showed a 96% sequence identity with and characteristics similar to that of Burkholderia cepacia. Analysis of phylogeny and BIOLOG confirmed that the strain WH1 was indeed B. cepacia. The bacteria grew well at 37°C in media containing 10 mM 3CP but exhibited a rather slow doubling time of 43.62 h, with an optimum chloride ion release of 0.194 µmol Cl − /mL. Most importantly, analysis by high perfomance chromatography revealed that the B. cepacia isolate effectively degraded ~100% of available 10 mM 3CP. This is the first report detailing a B. cepacia strain able to competently utilize 3CP as its sole carbon source.

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“…These approaches may also include works in the improvement of catalytic properties of known enzymes through protein engineering, either through molecular biology or direct protein modification (Hamid et al, 2011). Moreover, chances of finding enzymes with extraordinary properties is fairly reasonable considering numerous reports pertaining to microbial isolation studies (Jing et al, 2008;Roslan et al, 2011;Abel et al, 2012b;Bagherbaigi et al, 2013;Niknam et al, 2014). Such findings have since open doors on explorations of different properties of dehalogenases from countless microorganisms capable of hydrolyzing 2,2-DCP (Jing et al, 2008;Roslan et al, 2011;Abel et al, 2012b;Bagherbaigi et al, 2013;Niknam et al, 2014).…”

Section: Introductionmentioning

confidence: 97%

Isolation and identification of bacteria isolated from ruminant animal waste that able to degrade 2,2-dichloropropionic acid (2,2-DCP)

Ismail¹,

Wahab²,

Huyop³

2016

MJM

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Aims: A 2,2-dichloropropionic acid (2,2-DCP) naturally degrading bacterial species, strain SN1 was successfully isolated from cow dung capable of utilizing the substance as the sole carbon source and energy. Methodology and results: Strain SN1 was preferred over other strains (SN2, SN3 and SN4) following observations on its rapid growth in 20 mM 2,2-DCP liquid minimal media. Since strain SN1 clearly exhibited tolerance towards 2,2-DCP, its growth in various concentrations (10 mM, 20 mM, 30 mM and 40 mM) of the substance was evaluated. The study found the bacteria grew particularly well in 20 mM 2,2-DCP with the highest chloride release of 39.5 µmole Cl-/mL while exhibiting a remarkably short doubling time of 3.85 h. In view of such notable characteristics, species identification via Biolog GEN III system and 16S rRNA analysis was performed and established strain SN1 as Bacillus cereus. Conclusion, significance and impact of study: Considering the rapid growth of B. cereus strain SN1 in such medium, its employment as a bioremediation agent to treat 2,2-DCP contaminated soils may prove beneficial. Moreover, this is the first reported case of a Bacillus sp. isolated from cow dung capable of utilizing 2,2-DCP. Therefore, further assessment into its ability to degrade other types of haloalkanoic acids merit special consideration.

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Characterisation of Arthrobacter sp. S1 that can degrade α and β-haloalkanoic acids isolated from contaminated soil

Cited by 18 publications

References 31 publications

Further Analysis of <i>Burkholderia pseudomallei</i> MF2 and Identification of Putative Dehalogenase Gene by PCR

Further Analysis of <i>Burkholderia pseudomallei</i> MF2 and Identification of Putative Dehalogenase Gene by PCR

Isolation and characterization of a biodegrading 3-chloropropionic acid Burkholderia cepacia WH1 isolated from abandoned agricultural land

Isolation and identification of bacteria isolated from ruminant animal waste that able to degrade 2,2-dichloropropionic acid (2,2-DCP)

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