Characterisation of bovine viral diarrhoea virus (BVDV) isolates from an outbreak with haemorrhagic enteritis and severe pneumonia

Yeşilbağ, Kadir; Förster, Christine; Özyiğit, M. Özgür; Alpay, Gizem; Tuncer, Pelin; Thiel, Heinz-Jürgen; König, Matthias

doi:10.1016/j.vetmic.2013.12.005

Cited by 38 publications

(27 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Initially, members of the BVDV-2 species were considered more virulent than BVDV-1 strains in general and the latter were believed unable to induce hemorrhagic disease. However, more detailed analyses revealed that infection of cattle with BVDV-2 can be inapparent or accompanied by only mild symptoms and, vice versa, BVDV-1 is able to induce hemorrhagic disease (Lunardi, Headley, Lisboa, Amude, & Alfieri, 2008;Yesilbag et al, 2014). A very special case of fatal disease resulting from infection with a mixture of BVDV-2 was described recently (see Section 5) ( Jenckel et al, 2014).…”

Section: Bovine Viral Diarrhea and Mucosal Diseasementioning

confidence: 99%

The Molecular Biology of Pestiviruses

Tautz

Tews

Meyers

2015

Advances in Virus Research

204

200

View full text Add to dashboard Cite

Section: Bovine Viral Diarrhea and Mucosal Diseasementioning

confidence: 99%

The Molecular Biology of Pestiviruses

Tautz

Tews

Meyers

2015

Advances in Virus Research

204

200

View full text Add to dashboard Cite

“…After the description of two BVDV-1 subgenotypes in the early 1990s [39], at least twenty-one BVDV-1 subgenotypes (BVDV-1a to -1u) and four BVDV-2 subgenotypes (BVDV-2a to -2d) have been described to date [21,81,49,90,106,121,136,160,161]. The phylogenetic tree based on the N pro coding sequences includes 18 BVDV-1 and two BVDV-2 subgenotypes; for the remaining BVDV-1 and BVDV-2 subgenotypes, complete N pro coding sequences are not available (Figure 1).…”

Section: Bvdv Variabilitymentioning

confidence: 99%

“…According to the literature, a few BVDV isolates could not be allocated into one of the known subgenotypes and, in rare cases, the same subgenotype name was used in different studies for the designation of various BVDV-1 subgenotypes [121,136,160,161]. Apparently, the main reason for this confusing situation is the lack of a harmonized system for segregation of BVDV strains into subgenotypes.…”

Section: Bvdv Variabilitymentioning

confidence: 99%

Variability and Global Distribution of Subgenotypes of Bovine Viral Diarrhea Virus

Yeşilbağ

Alpay

Becher

2017

Viruses

Self Cite

208

250

View full text Add to dashboard Cite

Bovine viral diarrhea virus (BVDV) is a globally-distributed agent responsible for numerous clinical syndromes that lead to major economic losses. Two species, BVDV-1 and BVDV-2, discriminated on the basis of genetic and antigenic differences, are classified in the genus Pestivirus within the Flaviviridae family and distributed on all of the continents. BVDV-1 can be segregated into at least twenty-one subgenotypes (1a–1u), while four subgenotypes have been described for BVDV-2 (2a–2d). With respect to published sequences, the number of virus isolates described for BVDV-1 (88.2%) is considerably higher than for BVDV-2 (11.8%). The most frequently-reported BVDV-1 subgenotype are 1b, followed by 1a and 1c. The highest number of various BVDV subgenotypes has been documented in European countries, indicating greater genetic diversity of the virus on this continent. Current segregation of BVDV field isolates and the designation of subgenotypes are not harmonized. While the species BVDV-1 and BVDV-2 can be clearly differentiated independently from the portion of the genome being compared, analysis of different genomic regions can result in inconsistent assignment of some BVDV isolates to defined subgenotypes. To avoid non-conformities the authors recommend the development of a harmonized system for subdivision of BVDV isolates into defined subgenotypes.

show abstract

“…Recently, a third BVDV species, called HoBi-like virus or BVDV3, was reported [4]. BVDV-1 is classified by genetic analysis into about 20 subtypes (1a-1t) [5][6][7][8][9][10] and BVDV-2 into three subtypes (2a-2c) [11][12][13]. BVDV subtyping is useful in molecular epidemiological studies, vaccine development and tracing the origin of emerging viruses [14].…”

Section: Introductionmentioning

confidence: 99%

Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt

Soltan

Wilkes

Elsheery

et al. 2015

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Bovine viral diarrhea (BVD) is one of the most economically significant diseases in the bovine industry causing losses due to diarrhea, reproductive disorders, immunosuppression and mortalities. The aim of our investigation was to detect and subtype BVDV from calves on two dairy cattle and two buffalo farms in Ismailia province, Egypt as an indicator of BVDV infection status in the province. Methodology: A total of 298 blood samples were collected and tested using an optimized one-step, real-time multiplex Taqman-based RT-PCR. All the positive samples by the multiplex real-time RT-PCR were tested using conventional RT-PCR to amplify multiple areas of the genome for further phylogenetic analysis and subtyping. Results: Thirty one (10.4%) of the tested samples were positive for BVDV-1. Only three samples, all from a single dairy cattle farm, had enough viral RNA to be amplified by RT-PCR. The PCR products were sequenced and phylogenetic analysis revealed detection of BVDV1b. The detected strain is closely related to worldwide BVDV-1b strains, making it difficult to trace its origin. Nucleotide and amino acid alignments of the E2 glycoprotein region of the detected strain with other BVDV-1b strains showed high divergence, with identity ranging from 81.3% to 93.6% and 85.3% to 93.6%, respectively. Conclusion: To our knowledge, this is the first report describing the circulation of BVDV-1b in Egyptian dairy cattle populations.

show abstract

Characterisation of bovine viral diarrhoea virus (BVDV) isolates from an outbreak with haemorrhagic enteritis and severe pneumonia

Cited by 38 publications

References 22 publications

The Molecular Biology of Pestiviruses

The Molecular Biology of Pestiviruses

Variability and Global Distribution of Subgenotypes of Bovine Viral Diarrhea Virus

Circulation of bovine viral diarrhea virus – 1 (BVDV-1) in dairy cattle and buffalo farms in Ismailia Province, Egypt

Contact Info

Product

Resources

About