Characterisation of cellulase activity in the digestive system of the redclaw crayfish (Cherax quadricarinatus)

Xue, Xiao M; Anderson, Alys J; Richardson, Neil; Anderson, Alan J.; Xue, Gang; Mather, Peter B.

doi:10.1016/s0044-8486(99)00213-6

Cited by 86 publications

(79 citation statements)

References 22 publications

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“…28A). These results correspond with the earlier reports suggesting that decapods have endogenous cellulase enzymes (Xue et al 1999;Byrne et al 1999;Linton et al 2006;Linton and Greenaway 2007). Lichenin is a polysaccharide mainly found in lichen.…”

Section: -1 Cellulase and Hemicellulase Distribution In Wetlandssupporting

confidence: 92%

Degradation of Plant-derived Carbohydrates in Wetlands

Ogino¹,

Liu²,

Toyohara³

2018

Aqua-BioSci. Monogr. (ABSM)

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Corbicula japonica is one of the most important bivalves in inland fishery resources. Stable isotopic studies have shown that it can assimilate plant-derived hard-degradable carbohydrates. Further studies revealed that this species has endogenous enzymes to digest these carbohydrates.We investigated the ability of various organisms to degrade hard-degradable carbohydrates in wetlands from subarctic to subtropical zones and found that they could contribute to their degradation.We found that the enzymes secreted from organisms would bind to the sediment and contribute to the degradation of cellulose. We defined these enzymes as "the environmental enzymes". Comparison of various sediments revealed that the binding abilities of the sediment for the environmental enzyme were ascribable to oxidized metal, organic matters and the ratio of sand, silt and clay.

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Section: -1 Cellulase and Hemicellulase Distribution In Wetlandssupporting

confidence: 92%

Degradation of Plant-derived Carbohydrates in Wetlands

Ogino¹,

Liu²,

Toyohara³

2018

Aqua-BioSci. Monogr. (ABSM)

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“…Mass spec indicates that the mass of the enzyme was determined by its mass spectrum on a time of flight mass spectrometer. cDna indicates the size of the enzyme predicted from the translated amino acid sequence -acetyl-β-d-glucosaminidase Penaeus vannamei (prawn) 105 (2 × 45 subunits) (denatured) Xie et al (2004) Scylla serrata (crab) 132 (2 × 65.8 subunits) (denatured) Zhang et al (2006) Penaeus japonicus (prawn) 37 (denatured), 57 (mass spec) Kono et al (1990) endo-β-1,4-glucanase Cherax quadricarinatus 30, 40 (native) Xue et al (1999) (2010) terrestrial, mainly leaf litter diet, particularly in the gecarcinids, G. natalis and D. celeste since these species possess isozymes that were not observed in the coenobitids. In particular, the gecarcinid crabs possessed additional endo-β-1,4-glucanase isozymes: G. natalis possessed two extra isoforms and D. celeste one.…”

Section: Discussionmentioning

confidence: 99%

Digestive enzymes of two brachyuran and two anomuran land crabs from Christmas Island, Indian Ocean

et al. 2014

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higher endo-β-1,4-glucanase, lichenase and laminarinase activities compared to that of the other species. Thus, C. perlatus may be efficient at digestion of cellulose and hemicellulose within plant material. Zymography indicated that the majority of protease, lipase, phosphatase, amylase, endo-β-1,4-glucanase, β-glucohydrolase and N-acetyl-β-d-glucosaminidase isozymes were common to all species, and hence were inherited from a common aquatic ancestor. Differences were observed for the phosphatase, lipase and endo-β-1,4-glucanase isozymes. These differences are discussed in relation to phylogeny and possible evolution to cope with the adoption of a terrestrial diet.

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“…Study of digestive enzymes is an essential step towards understanding the mechanism of digestion and how the organism adapts to changes in the nutritional environment (Xue et al 1999). The gut (endogenous) phytase enzyme is the indicator of digestibility and utilization of nutrients and minerals.…”

Section: Experimental Dietsmentioning

confidence: 99%

Comparative efficacy of phytase from fish gut bacteria and a commercially available phytase in improving the nutritive value of sesame oilseed meal in formulated diets for fingerlings of rohu, Labeo rohita (Actinopterygii: Cypriniformes: Cyprinidae)

Roy¹

2016

Acta Ichthyol. Piscat.

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. 2016. Comparative effi cacy of phytase from fi sh gut bacteria and a commercially available phytase in improving the nutritive value of sesame oilseed meal in formulated diets for fi ngerlings of rohu, Labeo rohita (Actinopterygii: Cypriniformes: Cyprinidae). Acta Ichthyol. Piscat. 46 (1): 9-23.Background. Phytate (myo-inositol 1,2,3,4,5,6-hexakis-dihydrogen phosphate) is the main storage form of phosphorus (P) and up to 80% of the total P content in plants remains unavailable to fi sh due to lack of intestinal phytases for effi cient phytate hydrolysis. The inclusion of microbial phytase in the feed is an approach to increase phytate phosphorus bioavailability. In the presently reported study, a comparison of the effi cacy of phytase produced by fi sh gut bacterial strain, Bacillus licheniformis LF1 and a commercially available phytase, Biophos-TS in improving the nutritive value of sesame (Sesamum indicum) oilseed meal (SOM) was evaluated in the diet for fi ngerlings of rohu, Labeo rohita (Hamilton, 1822). Materials and methods. Eleven isonitrogenous (approximately 35% crude protein) and isocaloric (17.58 kJ · g -1 gross energy) experimental diets were formulated with the same basal diet containing raw sesame oilseed meal (D1) or oilseed meal pretreated with phytase from the fi sh gut bacterial strain, B. licheniformis LF1 (D2-D6) and commercially available phytase, Biophos-TS (D7-D11) at 10, 20, 30, 40, and 50 FTU · kg -1 and fed to rohu fi ngerlings (mean initial weight 1.85 ± 0.52 g) for 80 days. Results. Fermentation of oilseed meal signifi cantly reduced the crude fi bre content and anti-nutritional factors tannins and phytic acid, and enhanced mineral concentration. In terms of growth, feed conversion ratio, and protein effi ciency ratio, diets containing sesame oilseed meal pretreated with Biophos-TS at a concentration of 30 FTU · kg -1 and with strain LF1 at a concentration of 40 FTU · kg -1 resulted in a signifi cantly (P < 0.05) better performance of rohu fi ngerlings. The apparent digestibility of protein, lipid, ash and minerals was signifi cantly (P < 0.05) higher in fi sh fed diets D9 and D5 in comparison to those fed reference diet (RD). The crude protein, crude lipid, and ash contents of fi sh carcass were highest in fi sh fed diet D9, which was not signifi cantly (P < 0.05) different from those in the group of fi sh fed diet D5. Pretreatment of diets with phytase reduced faecal P levels. Conclusion.Comparison of the effi cacy of phytase produced by fi sh gut bacteria with commercially available phytase indicated no signifi cant difference in performance of rohu fi ngerlings in terms of growth and nutrient and mineral utilization. Keywords: growth performance, mineral utilization, phytase, plant ingredient, aquafeed, Indian major carp INTRODUCTIONUnlike most domesticated farm animals, the majority of fi sh species generally require higher levels of dietary protein for optimum growth. High quality fi sh meal, with amino acid profi le that matched the fi sh's requirement pattern is therefore, ...

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Characterisation of cellulase activity in the digestive system of the redclaw crayfish (Cherax quadricarinatus)

Cited by 86 publications

References 22 publications

Degradation of Plant-derived Carbohydrates in Wetlands

Degradation of Plant-derived Carbohydrates in Wetlands

Digestive enzymes of two brachyuran and two anomuran land crabs from Christmas Island, Indian Ocean

Comparative efficacy of phytase from fish gut bacteria and a commercially available phytase in improving the nutritive value of sesame oilseed meal in formulated diets for fingerlings of rohu, Labeo rohita (Actinopterygii: Cypriniformes: Cyprinidae)

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