Characterisation of Chitosan from Blowfly Larvae and Some Crustacean Species from Kenyan Marin Waters Prepared Under Different Conditions

Oduor‐Odote, Peter; Struszczyk, Marcin H.; Peter, Martin G.

doi:10.4314/wiojms.v4i1.28478

Cited by 16 publications

(18 citation statements)

References 12 publications

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“…The yield of chitosan produced from extracted chitin was 77.78%, which was similar to the results that was reported in the literature by Kaya et al (76% yield of chitosan from Callinectes sapidus from İskenderun, Turkey) and Odote et al (74.6% yield of chitosan of Sylla cerrata from Mombasa, Kenya) [13,14]. The yields were above average in these studies and our study indicated that crabs are one of the major resources of chitin and chitosan among the other crustacean group of organisms.…”

Section: Chitin Extraction and Chitosan Productionsupporting

confidence: 88%

Extraction and Characterization of Chitin and Chitosan from Blue Crab and Synthesis of Chitosan Cryogel Scaffolds

Bölgen¹,

Demir²,

Öfkeli³

et al. 2016

J. Turk. Chem. Soc., Sect. A: Chem.

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Polymeric scaffolds produced by cryogelation technique have attracted increasing attention for tissue engineering applications. Cryogelation is a technique which enables to produce interconnected porous matrices from the frozen reaction mixtures of polymers or monomeric precursors. Chitosan is a biocompatible, biodegradable, nontoxic, antibacterial, antioxidant, and antifungal natural polymer that is obtained by deacetylation of chitin, which is mostly found in the exoskeleton of many crustaceans. In this study, chitin was chemically isolated from the exoskeleton of blue crab (Callinectes sapidus). Callinectes sapidus samples were collected from a market, as a waste material after it has been consumed as food. Demineralization, deproteinization, and decolorization steps were applied to the samples to obtain chitin. Chitosan was prepared from isolated chitin by deacetylation at high temperatures. The chemical composition of crab shell, extracted chitin and chitosan were characterized with FTIR analysis. Moreover, in order to determine the physicochemical and functional properties of the produced chitosan, solubility, water uptake, and oil uptake analysis were performed. Chitosan cryogel scaffolds were prepared by crosslinking reaction at cryogenic conditions at constant amount of chitosan (1%, w/v) with different ratios of glutaraldehyde (1, 3, and 6%, v/v) as crosslinker. The chemical structure of the scaffolds were examined by FTIR. Also, the water uptake capacity of scaffolds have been determined. Collectively, the results suggested that the characterized chitosan cryogels can be potential scaffolds to be used in tissue engineering applications.

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Section: Chitin Extraction and Chitosan Productionsupporting

confidence: 88%

Extraction and Characterization of Chitin and Chitosan from Blue Crab and Synthesis of Chitosan Cryogel Scaffolds

Bölgen¹,

Demir²,

Öfkeli³

et al. 2016

J. Turk. Chem. Soc., Sect. A: Chem.

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“…In the next step, chitosan was produced from the extracted chitin at 80.23 %. This yield is higher than the data reported in the literature (Oduor-Odeto et al, 2005;Kaya et al, 2016;Demir et al, 2016;Varun et al, 2017;Ahyat et al, 2017) and the result proved that the crabs are one of the significant resources of chitin and chitosan among the other crustacean organisms. It's noteworthy that the obtained chitin and chitosan can be used as biomaterial.…”

Section: Discussioncontrasting

confidence: 52%

Extraction and Characterization of Chitin and Chitosan from Invasive Alien Swimming Crab Charybdis longicollis

Doğdu¹,

Turan²,

Depçi̇³

2021

Natural and Engineering Sciences

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Chitosan is a biocompatible, biodegradable, non-toxic, antibacterial, antioxidant, and antifungal natural polymer. In this study, chitin and chitosan were chemically isolated from the exoskeleton of invasive swimming crab Charybdis longicollis. In order to obtain the chitin, demineralization, deproteinization, and decolourization steps were applied to the samples. Chitosan was prepared from the isolated chitin by deacetylation at high temperatures. The chemical composition of chitin from C. longicollis was characterized by XRD and FTIR analysis. The yield of chitin extraction from dry crab shells was 25.78 %. The yield of chitosan produced from extracted chitin was 80.23 %. The experimental analyses revealed that the obtained chitin and chitosan could be used as biomaterial.

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“…In contrast, the cuticle of insects consists of chitin in a matrix with lipids, cuticular proteins, and additional compounds [ 35 ]. Most insects contain insignificant amounts of minerals in their cuticle, so the demineralization process was not required [ 36 , 37 ]. The average molecular weights of chitosan extracted from C. albiceps and S. aegyptiaca were lower than those of commercial chitosan.…”

Section: Discussionmentioning

confidence: 99%

Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells

Rahman

Ghaffar

Alkhuriji

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Traditional folk therapies indicate that insects have diverse medicinal potentials. However, the therapeutic application of insect chitosan and its derivatives has not been explored. To investigate the application of chitosan and its derivatives, the carboxymethyl derivative of chitosan (CM-Ch) was extracted from two dipteran larvae species, Chrysomya albiceps and Sarcophaga aegyptiaca. The degree of deacetylation (DD) and CM-Ch functional groups were validated using Fourier-transform infrared (FTIR) spectroscopy analysis and proton nuclear magnetic resonance spectroscopy (1H NMR), respectively. The molecular weight was estimated using MALDI-TOF MS analysis. The effect of CM-Ch on the morphology and proliferation of human liver HepG2 cancer cells was assessed. IC50 of CM-Ch induced significant growth-inhibitory effects in HepG2 cells. CM-Ch treatment altered the morphology of HepG2 in a dose-dependent manner and induced apoptosis in a caspase-dependent manner. CM-Ch treatment showed no signs of toxicity, and no alterations in liver and kidney biochemical markers were observed in albino rats. A CM-Ch derivative from commercial crustacean chitosan was used to assess the efficacy of the insect-derived CM-Ch. The data presented here introduce insect CM-Ch as a promising, inexhaustible, safe derivative of chitosan with antitumor potential in liver cancer. This is the first report highlighting the anticancer activity of insect CM-Ch in hepatocellular carcinoma cells.

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Characterisation of Chitosan from Blowfly Larvae and Some Crustacean Species from Kenyan Marin Waters Prepared Under Different Conditions

Cited by 16 publications

References 12 publications

Extraction and Characterization of Chitin and Chitosan from Blue Crab and Synthesis of Chitosan Cryogel Scaffolds

Extraction and Characterization of Chitin and Chitosan from Blue Crab and Synthesis of Chitosan Cryogel Scaffolds

Extraction and Characterization of Chitin and Chitosan from Invasive Alien Swimming Crab Charybdis longicollis

Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells

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