Characterisation of chlorate reduction in the haloarchaeon Haloferax mediterranei

Martínez‐Espinosa, Rosa María; Richardson, David J.; Bonete, Marı́a José

doi:10.1016/j.bbagen.2014.12.011

Cited by 52 publications

(66 citation statements)

References 39 publications

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“…The review by Bernardi et al (75) addresess sampling methods and techniques for MD simulations of biological systems. Methods to apply MD simulations to small peptides and biological macromolecules are reviewed by Doshi et al (76). The use of short time trajectory fragments to enhance sampling of kinetics is discussed in the review by Elber et al (77).…”

Section: Simulation Methodsmentioning

confidence: 99%

Review of the fundamental theories behind small angle X-ray scattering, molecular dynamics simulations, and relevant integrated application

2015

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In this paper, the fundamental concepts and equations necessary for performing small angle X-ray scattering (SAXS) experiments, molecular dynamics (MD) simulations, and MD-SAXS analyses were reviewed. Furthermore, several key biological and non-biological applications for SAXS, MD, and MD-SAXS are presented in this review; however, this article does not cover all possible applications. SAXS is an experimental technique used for the analysis of a wide variety of biological and non-biological structures. SAXS utilizes spherical averaging to produce one- or two-dimensional intensity profiles, from which structural data may be extracted. MD simulation is a computer simulation technique that is used to model complex biological and non-biological systems at the atomic level. MD simulations apply classical Newtonian mechanics’ equations of motion to perform force calculations and to predict the theoretical physical properties of the system. This review presents several applications that highlight the ability of both SAXS and MD to study protein folding and function in addition to non-biological applications, such as the study of mechanical, electrical, and structural properties of non-biological nanoparticles. Lastly, the potential benefits of combining SAXS and MD simulations for the study of both biological and non-biological systems are demonstrated through the presentation of several examples that combine the two techniques.

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Section: Simulation Methodsmentioning

confidence: 99%

Review of the fundamental theories behind small angle X-ray scattering, molecular dynamics simulations, and relevant integrated application

2015

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“…The ability of NarGH to use (per)chlorate as final electron acceptor in the absence of oxygen is due the location of its active site facing the membrane potential positive face (pNars) (Martinez-Espinosa et al, 2007;Martínez-Espinosa et al, 2015). It permits to reduce these anions to chlorite with any intracellular damage.…”

Section: The Use Of Haloferax Mediterranei In Wastewater Treatmentsmentioning

confidence: 99%

“…In general terms, Haloferax growth under those microaerobic or even strict anaerobic conditions is possible because oxygen is replaced by other final electron acceptors such us nitrate, nitrite (Lledó, Martinez-Espinosa, Marhuenda-Egea & Bonete, 2004;Bonete et al, 2008;Nájera-Fernández, Zafrilla, Bonete & Martinez-Espinosa, 2012;Esclapez, Zafrilla, Martinez-Espinosa & Bonete, 2013) (per)chlorate (Oren, Elevi-Bardavid & Mana, 2014; Martínez-Espinosa, Richardson & Bonete, 2015), sulphur or sulphide (Elshahed et al, 2004), arsenate (Rascovan, Maldonado, Vazquez & Eugenia Farias, 2015), dimethyl sulfoxide (DMSO), trimethylamine N-oxide (TMAO) and fumarate (Oren & Trüper, 1990;Oren, 1991;Oren, 1999;Müller & DasSarma, 2005).…”

Section: Anaerobic Metabolism In the Haloferax Genusmentioning

confidence: 99%

Anaerobic Metabolism in Haloferax Genus

Torregrosa-Crespo

Martínez‐Espinosa

Esclapez

et al. 2016

Advances in Microbial Physiology

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A number of species of Haloferax genus (halophilic Archaea) are able to grow micro aerobically or even anaerobically using different alternative electron acceptors such as fumarate, nitrate, chlorate, dimethyl sulphoxide, sulphide and/or trimethylamine. This metabolic capability is also shown by other species of the Halobacteriaceae and Haloferacaceae families (Archaea domain) and it has been mainly tested by physiological studies where cells growth is observed under anaerobic conditions in the presence of the mentioned compounds. This work summarises the main reported features on anaerobic metabolism in the Haloferax, one of the better described haloarchaeal genus with significant potential uses in Biotechnology and Bioremediation. Special attention has been paid to denitrification, also called nitrate respiration. This pathway has been studied so far from KEY WORDSHaloarchaea, denitrification, anaerobic metabolism, Nox emissions, bioremediation.

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“…Most isolated (per)chlorate-reducers belong to the phyla Proteobacteria. Archaeal (per)chlorate reduction, only recently discovered, appears to involve periplasmic nitrate reductase (pNar) instead of Pcr or Clr [5,6]. All three enzymes are molybdenum-dependent members of the DMSO reductase superfamily; therefore all known (per)chlorate-reducing microorganisms require trace amounts of molybdenum [2,7].…”

Section: Chloroxyanion Respirationmentioning

confidence: 99%

“…The archaeon Haloferax mediterranei, which contains a narG-type nitrate reductase, can grow by chlorate reduction for several generations if pregrown by nitrate reduction [6], and some Haloferax strains can grow by the aerobic oxidation of hydrocarbons, but anaerobic oxidation of hydrocarbons has not been demonstrated for extreme halophiles under any electron accepting conditions [22].…”

Section: Mesophiles Marinementioning

confidence: 99%

Enrichment and Isolation of Chloroxyanion-Respiring Hydrocarbon Oxidizers

Barnum

Coates

2016

Springer Protocols Handbooks

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Converging lines of research point to chloroxyanion-respiring hydrocarbon oxidizers as an important group of microorganisms that merit expanded isolation and characterization. Respiration of the chloroxyanions perchlorate (ClO 4 À ) and chlorate (ClO 3 À ), collectively termed (per)chlorate, is the only non-phototrophic process shown to produce molecular oxygen (O 2 ). Accordingly, strains that respire (per)chlorate while oxidizing hydrocarbons have been of longstanding interest for bioremediation of hydrocarbons in anaerobic environments (Coates et al., Nature 396 (6713) This protocol provides an overview of anaerobic culturing and handling hydrocarbons (reviewed extensively in Davidova and Sulfita, Methods Enzymol 397(05):17-34, 2005) and specific techniques for the enrichment and isolation of chloroxyanion-respiring hydrocarbon oxidizers from several environments of interest.

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Characterisation of chlorate reduction in the haloarchaeon Haloferax mediterranei

Cited by 52 publications

References 39 publications

Review of the fundamental theories behind small angle X-ray scattering, molecular dynamics simulations, and relevant integrated application

Review of the fundamental theories behind small angle X-ray scattering, molecular dynamics simulations, and relevant integrated application

Anaerobic Metabolism in Haloferax Genus

Enrichment and Isolation of Chloroxyanion-Respiring Hydrocarbon Oxidizers

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