Characterisation of coagulase positive Staphylococcus species isolated from bovine mastitis using protein and plasmid patterns

Arslan, Emine; Çelebi, Ayten; Açık, Leyla; Uçan, Uçkun Sait

doi:10.3906/vet-0808-8

Cited by 5 publications

(6 citation statements)

References 20 publications

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“…However, in contrast to our findings, other studies (Oncel et al, 2004;Paludi et al, 2011;Serieys & Gicquel-Bruneau, 2005) have reported a low rate of penicillin resistance. No vancomycin-resistant isolates were detected in the present study, which is consistent with numerous investigations (Ateba et al, 2010;Farzana et al, 2004;Gundogan et al, 2005;Gündo gan et al, 2006;Kirkan et al, 2005;Lee, 2003;Pesavento et al, 2007) and could be due to the infrequent use of vancomycin in veterinary medicine (Pace & Yang, 2006).…”

Section: F I G U R Esupporting

confidence: 93%

Rapid detection of major enterotoxin genes and antibiotic resistance of Staphylococcus aureus isolated from raw milk in the Yazd province, Iran

Forouzani‐Moghaddam,

Habibi,

Hosseini‐Safa

et al. 2024

Veterinary Medicine & Sci

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IntroductionRaw milk is a nutrient‐rich food, but it may harbour harmful bacteria, such as enterotoxigenic Staphylococcus aureus (S. aureus), which can cause staphylococcal food poisoning. Antibiotic resistance of S. aureus in raw milk can increase the risk of such infections, particularly among susceptible individuals.ObjectiveThis study aimed to investigate the prevalence of enterotoxin genes a, d, g, i and j and the antibiotic resistance of S. aureus isolated from raw milk samples.MethodsDuring a 6‐month sampling period, 60 raw milk specimens were obtained from diverse locations in Yazd province, Iran. Antibiogram profiling was conducted via the disc diffusion method. In addition, staphylococcal enterotoxin (SE) genes a, d, g, i, and j were detected through real‐time PCR analysis.ResultsBacteriological assays confirmed the presence of S. aureus in 11 samples (18.3%). All isolates demonstrated 100% resistance to penicillin G but exhibited sensitivity to vancomycin, while resistance to other antibiotics ranged from 36.4% to 45.5%. The prevalence of enterotoxin genes in these strains showed variable distribution, with sea being the predominant SE (45.5%), followed by sed (36.4%), seg (18.2), sej and sei (9.1% each).ConclusionsThis study discovered the presence of multiple enterotoxins in S. aureus strains obtained from raw milk samples. These strains also demonstrated resistance to a variety of antibiotics. Since enterotoxigenic S. aureus is known to cause human food poisoning, monitoring food hygiene practices, especially during raw milk production, is critical.

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Section: F I G U R Esupporting

confidence: 93%

Rapid detection of major enterotoxin genes and antibiotic resistance of Staphylococcus aureus isolated from raw milk in the Yazd province, Iran

Forouzani‐Moghaddam,

Habibi,

Hosseini‐Safa

et al. 2024

Veterinary Medicine & Sci

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“…Aslim and Beyatli [23] carried out a study on antibiotic resistance and plasmid DNA content of S. thermophilus, and showed that most of the plasmid-containing isolates were resistant to antibiotics, but has also shown sensitivity to some antibiotics too. Thus, isolates lacking plasmid DNA were susceptible to most antibiotics, yet a strain was found lacking plasmid DNA while being resistant to some of the antibiotics.…”

Section: Discussionmentioning

confidence: 99%

“…Akpinar et al [28] reported that most of the S. thermophilus isolates isolated from homemade yogurt samples were not found to be resistant towards all the tested antibiotics. According to Aslim and Beyatli [23], the resistance of S. thermophilus to antibiotics was not coded by plasmid DNAs and the genes of resistance were in the chromosomal DNA part.…”

Section: Discussionmentioning

confidence: 99%

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Probiotic properties and antibiotic susceptibility assessment of Streptococcus thermophilus isolates

Hamdaoui,

Azghar,

Benkirane

et al. 2023

Preprint

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Background Streptococcus thermophilus were considered one of probiotic bacterial strain; found in milk, cheese, and yogurt, that providing health benefits to humans. Due to their probiotic potentiality, S. thermophilus can be very helpful in replacing chemicals preservatives. This study came out to investigate the probiotic activity of ten S. thermophilus isolates obtained from raw cow milk in eastern Morocco against multidrug-resistant bacteria (MDR) bacteria: Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC: 25922 and 35218). Besides their antioxidant potential, bile salts tolerance as well as their antibiotic susceptibility, were evaluated Result The results showed remarkable in vitro inhibitory ability of the studied S. thermophilus isolates against the tested multidrug-resistant bacteria, as reported for S. thermophilus CFS and supernatant against Staphylococcus aureus (ATCC29213), confirming their potential as antibacterial agents. The mixtures of NaOH, catalase and proteinase K showed no inhibition zones against all tested multidrug-resistant bacteria; suggesting the main antibacterial effect of all isolates based on acids, hydrogen peroxide and/or proteins. For the antioxidant activity, the DPPH inhibition values varied between 18.97% and 44.44%, for the bacterial isolate ST9 and ST5, respectively. Notably, S. thermophilus isolates were able to survive up to 6h of incubation with a concentration of 0.1% of bile salt at 37°C. Thus, ST5 could be considered as the most promising bacterial isolate in terms of antioxidant activity. All isolates of S. thermophilus exhibited sensibility to all tested antibiotics except for rifampicin and trimethoprim-sulfamethoxazole compared to the standards proposed by the European committee on antimicrobial susceptibility testing. Conclusions The tested isolates were exhibited excellent antibacterial and potential antioxidant activities. In addition to that, these isolates were reported to resist rifampicin and trimethoprim-sulfamethoxazole antibiotics. These findings presage the use of S. thermophilus isolates as good probiotics.

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“…PCR primer sequences as shown in Table 1 [ [25] , [26] , [27] , [28] , [29] ] were purchased from Inqaba Biotec (Inqaba Biotec, Pretoria, South Africa). Successful PCR amplification of the species-specific thermonuclease ( nuc) gene was used to confirm the presence of S. aureus in the sample.…”

Section: Methodsmentioning

confidence: 99%

Molecular detection of virulence genes in Staphylococcus aureus isolated from wild pigeons (Columba domestica livia) in KwaZulu-Natal in South Africa

Wilson,

Zishiri,

El Zowalaty

2024

One Health

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Characterisation of coagulase positive Staphylococcus species isolated from bovine mastitis using protein and plasmid patterns

Cited by 5 publications

References 20 publications

Rapid detection of major enterotoxin genes and antibiotic resistance of Staphylococcus aureus isolated from raw milk in the Yazd province, Iran

Rapid detection of major enterotoxin genes and antibiotic resistance of Staphylococcus aureus isolated from raw milk in the Yazd province, Iran

Probiotic properties and antibiotic susceptibility assessment of Streptococcus thermophilus isolates

Molecular detection of virulence genes in Staphylococcus aureus isolated from wild pigeons (Columba domestica livia) in KwaZulu-Natal in South Africa

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