Characterisation of colloidal drug delivery systems from the naked eye to Cryo-FESEM

Nanoemulsions and Their Potential Applications in Food Industry

“…Cryo-TEM is effective in characterizing the structure of microemulsions (Spernath et al, 2009). The FFTEM provides detailed structure of bicontinuous nanoemulsions and droplets within discontinuous nanoemulsions (Krauel et al, 2007;Acharya and Hartley, 2012). Scanning electron microscope (SEM) is used to characterize materials in the nanometer to micrometer scale for microstructure morphology and chemical composition (Goldstein et al, 2003;Ferreira et al, 2015).…”

Glycol Chitosan‐Based Nanogel as a Potential Targetable Carrier for siRNA

“…The nanogel morphology under cryo‐FESEM was spherical as shown in Figure B, and the size distribution observed confirm the results obtained by dynamic light scattering (DLS). Cryo‐FESEM allows the observation of the nanogel close to its natural liquid state, preserving the three dimensional structure The stability of the nanogels in aqueous solution was studied over time by DLS. As observed in Figure D, both nanogels are stable for at least four months, which represents a high colloidal stability.…”

Subunit Vaccine Delivery

“…Plunge freezing in liquid propane has been shown to reduce ice crystal size to below the resolution of the microscope. Additionally, samples are generally sublimed for a few minutes to remove unwanted surface ice prior to coating (Krauel et al 2007 ).…”