Characterisation of erythrocyte shapes and sizes by NMR diffusion-diffraction of water: correlations with electron micrographs

Torres, Allan M.; Michniewicz, Radika J.; Chapman, Bogdan E.; Young, G. A. R.; Kuchel, Philip W.

doi:10.1016/s0730-725x(98)00005-8

Cited by 78 publications

(106 citation statements)

References 22 publications

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“…From conventional PFGSTE experiments, the minima were at q min1 ϭ 1.9 ϫ 10 5 m Ϫ1 and q min2 ϭ 3.7 ϫ 10 5 m Ϫ1 , whereas for the fast-measurement methods, these minima were at q min1 ϭ 2.0 ϫ 10 5 m Ϫ1 and q min2 ϭ 4.2 ϫ 10 5 m Ϫ1 . These values are related in a simple way to the mean diameter of the cells (8,12) and correspond to an apparent diameter of 6.4 m and 6.9 m, and 6.1 m and 5.8 m, respectively, for classical and fast-measurement experiments. The water restriction effect occurs for low residual water signal, that is, around 0.2% and 0.02% for the first and second minimum, respectively.…”

Section: Fast Diffusion-measurement Nmr Setupmentioning

confidence: 99%

“…The insets of Figure 6a-b show that the ATP concentration decreased immediately after adding NaF to the sample, whereas the fraction of the RBC population that was discocytes was initially unchanged and constant. The almost immediate decline of the ATP peaks is explained by the fluoride ion rapidly entering the cells and inhibiting enolase thus preventing ATP regeneration (12). Cell shape evolution was delayed to ϳ80 min at 25°C and ϳ40 min at 37°C, and it occurred when the ATP concentration inside the cells was low (ϳ0.5 mM).…”

Section: P Nmr and Fast-recording Nmr Diffusion-diffractionmentioning

confidence: 99%

“…A direct mathematical relationship exists between the position of these minima and the average mean diameter of the cells. It has already been shown that the PFGSTE NMR experiment is able to probe RBC shape (12) and, especially, to distinguish discocytes and spherocytes from their respective characteristic qspace plots; to characterize CNS structure (13)(14)(15)(16) and to study their pathologies (17)(18)(19).…”

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confidence: 99%

“…Due to the requirement to phase cycle the radiofrequency (RF) pulses it is necessary to record at least 16 transients per magnetic field gradient value. Therefore, the acquisition time for a typical q-space data set has been ϳ1 h (12). A few methods have been developed to decrease the experiment time (22) to record a diffusion experiment under "fast" conditions.…”

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confidence: 99%

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Erythrocyte‐shape evolution recorded with fast‐measurement NMR diffusion–diffraction

Pagès

Szekely

Kuchel

2008

Magnetic Resonance Imaging

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Purpose:To monitor red blood cell (RBC) shape evolution by 1 H 2 O diffusion-diffraction NMR in time steps comparable to those required for the acquisition of a 31 P NMR spectrum; thus, to correlate RBC mean diameter with ATP concentration after poisoning with NaF. Materials and Methods:Pulsed-field gradient-stimulated echo (PFGSTE) diffusion experiments were recorded on 1 H 2 O in RBC suspensions. Under conditions of restricted diffusion, q-space experiments report on mean RBC diameter. To decrease experiment time, the phase cycling of radiofrequency (RF) pulses was cut to two transients by using unbalanced pairs of gradient pulses. Data processing used a recent digital filter. Differential interference contrast (DIC) light microscopy also recorded shape changes. 31 P NMR spectroscopy gave estimates of mean ATP concentration.Results: NaF caused RBC-shape evolution from discocytes, through various forms of echinocytes, to spherocytes, over ϳ6 h and ϳ10 h at 37°C and 25°C, respectively. ATP declined to ϳ0.5 its normal concentration before the first stage of discocyte transformation; the concentration was 0.0 after ϳ1.5 h and 3.0 h, respectively, at the two temperatures. Conclusion:RBC shape was readily monitored by NMR with a temporal resolution that was useful for correlations with both DIC microscopy and 31 P NMR spectra.

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Section: Fast Diffusion-measurement Nmr Setupmentioning

confidence: 99%

Section: P Nmr and Fast-recording Nmr Diffusion-diffractionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Erythrocyte‐shape evolution recorded with fast‐measurement NMR diffusion–diffraction

Pagès

Szekely

Kuchel

2008

Magnetic Resonance Imaging

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“…QSI allows assessment of microarchitecture by exploiting the regularity of restrictions to molecular diffusion in porous systems as applied to determine pore geometry in porous solids (Callaghan et al, 1991), the study of packed erythrocytes (Torres et al, 1998), and the assessment of axonal architecture (Chin et al, 2004;Cohen and Assaf, 2002;Malmborg et al, 2006). In WM tracts, the porous system consists of water diffusion restricted by axon membranes and myelin sheaths (Beaulieu, 2002).…”

Section: Introductionmentioning

confidence: 99%

Indirect measurement of regional axon diameter in excised mouse spinal cord with q-space imaging: Simulation and experimental studies

et al. 2008

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Q-space imaging (QSI), a diffusion MRI technique, can provide quantitative tissue architecture information at cellular dimensions not amenable by conventional diffusion MRI. By exploiting regularities in molecular diffusion barriers, QSI can estimate the average barrier spacing such as the mean axon diameter in white matter (WM). In this work, we performed ex vivo QSI on cervical spinal cord sections from healthy C57BL/6 mice at 400MHz using a custom-designed uniaxial 50T/m gradient probe delivering a 0.6 µm displacement resolution capable of measuring axon diameters on the scale of 1 µm. After generating QSI-derived axon diameter maps, diameters were calculated using histology from seven WM tracts (dorsal corticospinal, gracilis, cuneatus, rubrospinal, spinothalamic, reticulospinal, and vestibulospinal tracts) each with different axon diameters. We found QSI-derived diameters from regions drawn in the seven WM tracts (1.1 to 2.1 µm) to be highly correlated (r 2 = 0.95) with those calculated from histology (0.8 to 1.8 µm). The QSI-derived values overestimated those obtained by histology by approximately 20%, which is likely due to the presence of extracellular signal. Finally, simulations on images of synthetic circular axons and axons from histology suggest that QSI-derived diameters are informative despite diameter and axon shape variation and the presence of intra-cellular and extra-cellular signal. QSI may be able to quantify nondestructively changes in WM axon architecture due to pathology or injury at the cellular level.

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Structural information in neuronal tissue as revealed byq-space diffusion NMR spectroscopy of metabolites in bovine optic nerve

Assaf

Cohen

1999

NMR Biomed.

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Characterisation of erythrocyte shapes and sizes by NMR diffusion-diffraction of water: correlations with electron micrographs

Cited by 78 publications

References 22 publications

Erythrocyte‐shape evolution recorded with fast‐measurement NMR diffusion–diffraction

Erythrocyte‐shape evolution recorded with fast‐measurement NMR diffusion–diffraction

Indirect measurement of regional axon diameter in excised mouse spinal cord with q-space imaging: Simulation and experimental studies

Structural information in neuronal tissue as revealed byq-space diffusion NMR spectroscopy of metabolites in bovine optic nerve

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