Characterisation of Escherichia coli strains involved in transcytosis across gut epithelial cells exposed to metabolic and inflammatory stress

Macutkiewicz, Christian; Carlson, Gordon L; Clark, E. C.; Dobrindt, Ulrich; Roberts, Ian S.; Warhurst, Geoffrey

doi:10.1016/j.micinf.2008.01.001

Cited by 34 publications

(30 citation statements)

References 32 publications

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“…In vitro studies suggest that the ability of ExPEC to translocate across the intestinal mucosa is strain-specific and related to localised inflammatory and metabolic stressors (54). A collection of small association studies have attempted to establish the role of particular VFs associated with intestinal translocation and have all demonstrated that a collection, as opposed to individual VFs, are likely to be related to these events (55)(56)(57).…”

Section: Vfs Associated With Bacteraemia and Gut Translocationmentioning

confidence: 99%

Extra-intestinal pathogenic Escherichia coli (ExPEC): Disease, carriage and clones

Dale

Woodford

2015

Journal of Infection

165

132

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Extra-intestinal pathogenic E. coli (ExPEC) have a complex phylogeny, broad virulence factor (VF) armament and significant genomic plasticity, and are associated with a spectrum of host infective syndromes ranging from simple urinary tract infection to life-threatening bacteraemia. Their importance as pathogens has come to the fore in recent years, particularly in the context of the global emergence of hyper-virulent and antibiotic resistant strains.Despite this, the mechanisms underlying ExPEC transmission dynamics and clonal selection remain poorly understood. Large-scale epidemiological and clinical studies are urgently required to ascertain the mechanisms underlying these processes to enable the development of novel evidence-based preventative and therapeutic strategies. In the current review, we provide a concise summary of the methods utilised for ExPEC phylogenetic genetic delineation before exploring in detail the associations between ExPEC VFs and site-specific disease. We then consider the role of ExPEC as an intestinal colonist before outlining known associations between ExPEC clonal variation, specific disease syndromes and antibiotic resistance.

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Section: Vfs Associated With Bacteraemia and Gut Translocationmentioning

confidence: 99%

Extra-intestinal pathogenic Escherichia coli (ExPEC): Disease, carriage and clones

Dale

Woodford

2015

Journal of Infection

165

132

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“…The method for this assay was developed from the technique described by Macutkiewicz et al (2008). Epithelial cells were cultured to confluence (~7 days) and treated with fresh medium 24 h in advance of the assay.…”

Section: Bacterial Internalisation Assaymentioning

confidence: 99%

“…As mentioned previously, past studies have utilised E. coli C25 as a model strain for bacterial translocation across the intestinal epithelium (Clark et al, 2003;Macutkiewicz et al, 2008); therefore, we sought to investigate the regulatory ability of OMVs on this process. To explore this, we performed a bacterial internalisation assay in both HT29-19A and Caco-2 cells.…”

Section: Pre-incubation With E Coli C25-derived Omvs Inhibits the Inmentioning

confidence: 99%

“…An early example of a commensal bacterium is E. coli C25 which was originally isolated from the faeces of a healthy individual in the mid-1950s (Freter and Hentges, 1956) and was subsequently demonstrated to have antagonistic activities against the enteric pathogen, Shigella flexneri (Hentges and Freter, 1962;Freter, 1962). Also, C25 lacks the traditional virulence genes found in pathogenic strains of E. coli, such as extraintestinal pathogenic (ExPEC), enterohaemorrhagic (EHEC) and enteropathogenic (EPEC) (Zareie et al, 2005;Macutkiewicz et al, 2008); moreover, it is a poor recipient of plasmid transfer (Freter and Brickner, 1983), so is unlikely to acquire such genes from other bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, studies utilising live C25 have demonstrated its ability to translocate through the intestinal epithelial barrier (Clark et al, 2003;Macutkiewicz et al, 2008;Suzuki and Okada, 2014) and to initiate a proinflammatory response in intestinal epithelial cell lines (Michalsky et al, 1997;Clark et al, 2003;Zareie et al, 2005;Macutkiewicz et al, 2008). …”

Section: Introductionmentioning

confidence: 99%

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Commensal-derived OMVs elicit a mild proinflammatory response in intestinal epithelial cells

et al. 2017

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Additionally, we show that removal of outer membrane vesicles (OMVs) diminishes the proinflammatory effect of secreted products from E. coli C25. Furthermore, we show that isolated OMVs have a dose-dependent proinflammatory effect on IECs. Interestingly, a relatively high concentration (10x culture concentration) of OMVs had no significant regulatory effects on TLR mRNA expression in both cell lines. Finally, we also demonstrate a that pre-incubation with E. coli C25-derived OMVs subsequently inhibited the internalisation of the bacterium itself in both cell lines. Taken together, our results suggest that commensal-derived extracellular products, in particular OMVs, could significantly contribute to intestinal homeostasis. We also demonstrate a unique interaction between commensal-derived OMVs and host cells.

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Metagenomic analysis of primary colorectal carcinomas and their metastases identifies potential microbial risk factors

et al. 2021

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The paucity of microbiome studies at intestinal tissues has contributed to a yet limited understanding of potential viral and bacterial co-factors of colorectal cancer (CRC) carcinogenesis or progression. We analyzed whole-genome sequences of CRC primary tumours, their corresponding metastases and matched normal tissue for sequences of viral, phage and bacterial species. Bacteriome analysis showed Fusobacterium nucleatum, Streptococcus sanguinis, F. Hwasookii, Anaerococcus mediterraneensis and further species enriched in primary CRCs.The primary CRC of one patient was enriched for F. alocis, S. anginosus, Parvimonas micra and Gemella sp. λ48. Enrichment of Escherichia coli strains IAI1, SE11, K-12 and M8 was observed in metastases together with coliphages enterobacteria phage φ80 and Escherichia phage VT2φ_272. Virome analysis showed that phages were the most preponderant viral species (46%), the main families being Myoviridae, Siphoviridae and Podoviridae. Primary CRCs were enriched for bacteriophages, showing five phages (Enterobacteria, Bacillus, Proteus, Streptococcus phages) together with their pathogenic hosts in contrast to normal tissues. The most frequently detected, and Blast-confirmed, viruses included human endogenous retrovirus K113, human herpesviruses 7 and 6B, Megavirus chilensis, cytomegalovirus (CMV) and Epstein-Barr virus (EBV), with one patient showing EBV enrichment in primary tumour and metastases. EBV was PCR-validated in 80 pairs of CRC primary tumour and their corresponding normal tissues; in 21 of these pairs (26.3%), it was detectable in primary tumours only. The number of viral species was increased and bacterial species decreased in CRCs compared with normal tissues,and we could discriminate primary CRCs from metastases and normal tissues by applying the Hutcheson t-test on the Shannon indices based on viral and bacterial species. Taken together, our results descriptively support hypotheses on microorganisms as potential (co-)risk factors of CRC, and extend putative suggestions on critical microbiome species in CRC metastasis.

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Characterisation of Escherichia coli strains involved in transcytosis across gut epithelial cells exposed to metabolic and inflammatory stress

Cited by 34 publications

References 32 publications

Extra-intestinal pathogenic Escherichia coli (ExPEC): Disease, carriage and clones

Extra-intestinal pathogenic Escherichia coli (ExPEC): Disease, carriage and clones

Commensal-derived OMVs elicit a mild proinflammatory response in intestinal epithelial cells

Metagenomic analysis of primary colorectal carcinomas and their metastases identifies potential microbial risk factors

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