Characterisation of hydrolysable tannins from leaves of Betula pubescens by high-performance liquid chromatography–mass spectrometry

Salminen, Juha‐Pekka; Ossipov, Vladimir; Loponen, Jyrki; Haukioja, Erkki; Pihlaja, Kalevi

doi:10.1016/s0021-9673(99)01036-5

Cited by 152 publications

(167 citation statements)

References 11 publications

Supporting

Mentioning

149

Contrasting

Unclassified

Order By: Relevance

“…Individual phenolics in the fraction of polar compounds (0.1 ml) were studied by using the high-performance liquid chromatography (HPLC) diode-array detection system as in Salminen et al (1999), except that the solvents used were (1) 0.05 M of H 3 PO 4 , and (2) acetonitrile. A selected set of samples was also analyzed with HPLC electrospray ionization mass spectrometry as in Salminen et al (1999), except that the data system used was Analyst Software 1.1.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Foliar Phenolics are Differently Associated with Epirrita autumnata Growth and Immunocompetence

et al. 2007

View full text Add to dashboard Cite

The quality of available food may affect insect herbivores directly (via growth and survivorship) and/or indirectly (by modifying insect vulnerability to parasitoids and pathogens). We examined the relationship between different phenolic compounds, belonging to various phenolic groups, in Betula pubescens spp. czerepanovii (mountain birch) foliage and the larval performance of the geometrid Epirrita autumnata (autumnal moth). Direct effects on insect performance were described by pupal weight, developmental rate, and survivorship; indirect effects were described by the encapsulation rate of an implant inserted into the insect hemocoel, a commonly used way to describe insect immune defense. We found profound differences in the effects of different phenolic categories: several individual hydrolyzable tannins were associated positively with larval performance but negatively with level of immune defense, whereas flavonoid glycosides were inversely related to larval survival but showed no association with the larvae immune defense.

show abstract

Section: Methodsmentioning

confidence: 99%

“…A selected set of samples was also analyzed with HPLC electrospray ionization mass spectrometry as in Salminen et al (1999), except that the data system used was Analyst Software 1.1. Phenolics were identified on the basis of their UV, mass spectra, and retention times.…”

Section: Methodsmentioning

confidence: 99%

Foliar Phenolics are Differently Associated with Epirrita autumnata Growth and Immunocompetence

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The HPLC system (Merck-Hitachi, Tokyo, Japan) consisted of a pump L-7100, a diode array detector L-7455, a programmable autosampler L-7250, and an interface D-7000. Column and chromatographic conditions were as described earlier (Salminen et al, 1999), except that 0.1 M H 3 PO 4 was replaced with 0.05 M H 3 PO 4 . Lipophilic flavonoid aglycones were identified on the basis of their UV and mass spectra and retention times as reported in the literature (Valkama et al, 2003), and quantified (absolute amounts) as acacetins (flavonols and flavones) or naringenins (flavanones).…”

Section: Methodsmentioning

confidence: 99%

Defensive Effect of Surface Flavonoid Aglycones of Betula pubescens Leaves Against First Instar Epirrita autumnata Larvae

et al. 2004

View full text Add to dashboard Cite

Abstract-The surface of birch leaves contains glandular trichomes that secrete exudates containing flavonoid aglycones. We investigated the biological activities of white birch (Betula pubescens) leaf surface exudates against larvae of the autumnal moth, Epirrita autumnata, a common insect pest of birch. We found that tree-specific mortality (up to 100%) of first instar larvae correlated strongly with the tree-specific contents of surface flavonoid aglycones (r s = 0.905) in emerging leaves. We also found that first instars clearly preferred birch buds from which surface exudates had been removed. In addition, the duration of the first instar was shortened by 29%, and the weights and relative growth rates of first instars improved by 8% and 52%, respectively, as a result of removal of the exudates from their leaf diet. The correlation of tree-specific foliar contents of flavonoid aglycones, especially 5-hydroxy-4 ,7-dimethoxyflavanone, with changes in larval performance, suggests that flavonoid aglycones are responsible for the changes observed in first instar larval performance. The results show that chemical characteristics of birch leaves are effective against neonate E. autumnata larvae. However, the removal of leaf surface exudates from fully expanded leaves did not affect the leaf acceptance for the voracious fifth instars. This is probably a result of reduction in contents of flavonoid aglycones compared to those of emerging leaves.

show abstract

“…According to the literature, the main characteristic in the mass spectra of these compounds was the deprotonated molecula [M-H] - (m/z 483, 635, 787, 939, 785, 937) and the loss of one or more galloyl groups (152 mu) and/or gallic acid (170 mu) (Salminen et al, 1999;Zywicki et al, 2002) As far as we know, there was no information on the phenolic composition of C. sativa and R. micrantha flowers, but a few previous studies have been published dealing with the phenolic composition of F. ulmaria flowers from Russia Krasnov et al, 2009;Shilova et al, 2006), Poland (Fecka, 2009) and Austria (Pemp et al, 2007). Some differences were found in the F. ulmaria sample (flowers from Portugal) studied herein, including the presence of different compounds such as isorhamnetin glucoside derivatives and caffeic acid derivatives.…”

Section: Hydrolyzable Tanninsmentioning

confidence: 99%

Characterization of phenolic compounds in wild medicinal flowers from Portugal by HPLC–DAD–ESI/MS and evaluation of antifungal properties

Barros

Alves

Dueñas

et al. 2013

Industrial Crops and Products

View full text Add to dashboard Cite

In the present work, the phenolic compounds of Castanea sativa, Filipendula ulmaria and Rosa micrantha flowers from Northeastern Portugal were characterized by HPLC-DAD-ESI/MS. Furthermore, it was performed a screening of their antifungal potential against Candida species (C. albicans, C. glabrata, C. parapsilosis and C. tropicalis).C. sativa sample gave the highest amount of phenolic compounds (18973 ± 40 µg/g, fw)and hydrolysable tannins (14873 ± 110 µg/g). The highest amounts of phenolic acids (569 ± 20 µg/g) and flavonoids (6090 ± 253 µg/g) were obtained in F. ulmaria and R. micrantha samples, respectively. Hydrolysable tannins (e.g. tri and digalloyl HHDP glucose) were the main group of phenolic compounds in C. sativa and F. ulmaria samples, while flavonoids (e.g. (+)-catechin and procyanidin dimers and trimers) were the most abundant group in R. micrantha. Thus, the stronger effect showed by this latter against all the Candida species (MIC ≤ 0.155 mg/mL) and, particularly its fungicide effects in C. glabrata, might be related to the mentioned flavonoids that were inexistence in the other samples.

show abstract

Characterisation of hydrolysable tannins from leaves of Betula pubescens by high-performance liquid chromatography–mass spectrometry

Cited by 152 publications

References 11 publications

Foliar Phenolics are Differently Associated with Epirrita autumnata Growth and Immunocompetence

Foliar Phenolics are Differently Associated with Epirrita autumnata Growth and Immunocompetence

Defensive Effect of Surface Flavonoid Aglycones of Betula pubescens Leaves Against First Instar Epirrita autumnata Larvae

Characterization of phenolic compounds in wild medicinal flowers from Portugal by HPLC–DAD–ESI/MS and evaluation of antifungal properties

Contact Info

Product

Resources

About