Characterisation of hyper tolerant Bacillus firmus L-148 for arsenic oxidation

Bagade, Aditi V.; Nandre, Vinod S.; Paul, Dhiraj; Patil, Yugendra R.; Sharma, Nisha; Giri, Ashok P.; Kodam, Kisan M.

doi:10.1016/j.envpol.2020.114124

Cited by 32 publications

(6 citation statements)

References 71 publications

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“…As a matter of fact, the recovery of Bacillus from copiotrophic and oligotrophic media and its survivability in a wide spectrum of salinity and nutrients unequivocally establishes its ubiquity in various habitats (Lazar and Dumitru, 1973 ; Laiz et al, 2000 , 2002a , b ; Saiz-Jimenez and Laiz, 2000 ). A review of the past literature (Incerti et al, 1997 ; Joshi et al, 2008 ; Antony et al, 2010 , 2013 , 2014 ; Surakasi et al, 2010 ; Paul et al, 2016 ; Bagade et al, 2020 ) indicates that the closest relatives of our isolates such as Nitrinicola, Alishwanella, Micrococcus, Halomonas , and Vibrio were detected by culture-independent approach. This study demonstrated that they adapted to grow at higher alkalinity and salinity, and therefore they dominated in the Lonar Lake ecosystem.…”

Section: Discussionmentioning

confidence: 72%

“…In this study, the habitat, i.e., Lonar Lake, is a highly alkaline and saline soda lake. Because of its uniqueness, several studies related to geochemistry, cultivation, and characterization of microbial diversity from sediment and water samples of Lonar Lake have been carried out (Joshi et al, 2008;Antony et al, 2010Antony et al, , 2013Antony et al, , 2014Surakasi et al, 2010;Paul et al, 2016;Bagade et al, 2020). However, there are no reports of oligotrophs/copiotrophic populations dominating the hypersaline and hyper-alkaline Lonar Lake ecosystem and their relation to geochemical features of the site, which may play cryptic roles in promoting them.…”

Section: Discussionmentioning

confidence: 99%

“…Sediment samples used for cultivation and enumeration studies were collected from the hyper-alkaline and hypersaline Lonar Lake located in Buldhana district, Maharashtra, India (19 • 59 ′ N, 76 • 31 ′ E), using the grab sampling method. The sampling site's geographic location and physiochemical features are described anywhere else (Joshi et al, 2008;Antony et al, 2010Antony et al, , 2013Antony et al, , 2014Surakasi et al, 2010;Borul, 2012;Paul et al, 2016;Bagade et al, 2020). For microbiological investigation, sediment samples from different locations of Lonar Lake were aseptically collected in pre-sterilized Falcon tubes, stored immediately on ice, and subsequently transported to the laboratory within 24 h. For cultivation and quantification purposes, samples were stored at 4 • C and processed within 4 days.…”

Section: Site Description and Samplingmentioning

confidence: 99%

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Oligotrophy vs. copiotrophy in an alkaline and saline habitat of Lonar Lake

et al. 2022

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We reported our comparative observations on oligotrophs vs. copiotrophs from a hyper-alkaline and hypersaline habitat, Lonar Lake, situated in the Buldhana district of Maharashtra, India. Cell numbers of oligotrophic and copiotrophic microbes from the sediment were enumerated by the three-tube most probable number (MPN) method using an array of nutrient-rich and oligotrophic (≈10–20 mg carbon L−1) media offering simulated natural conditions of pH and salinity. A total of 50 strains from 15 different genera and 30 different species were isolated from the highest positive dilutions of MPN to identify the taxa of oligotrophs and copiotrophic microorganisms dominating in Lonar Lake. We did not get any true oligotrophs due to their adaptation to higher carbon levels during the isolation procedure. On the contrary, several true copiotrophs, which could not adapt and survive on a low-carbon medium, were isolated. It is also observed that changes in medium composition and nutrient level altered the selection of organisms from the same sample. Our data indicate that copiotrophic microorganisms dominate the eutrophic Lonar Lake, which is also supported by the past metagenomics studies from the same site. We also reported that quick depletion of carbon from oligotrophic medium worked as a limiting factor, inducing cell death after 2–3 generations and preventing the development of visible colonies on plates and sufficient optical density in liquid medium. Therefore, a long-term supply of low levels of carbon, followed by isolation on enriched media, can serve as a good strategy in isolation of novel taxa of microorganism, with industrial or environmental importance.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Site Description and Samplingmentioning

confidence: 99%

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Oligotrophy vs. copiotrophy in an alkaline and saline habitat of Lonar Lake

et al. 2022

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“…Perhaps one of the most remarkably arsenic-tolerant bacterial strains isolated so far is a strain of Bacillus firmus isolated from soil near the Lonar lake in India [ 78 ]. The strain, characterized as Bacillus firmus L-148, showed exceptional tolerance to exceedingly high concentrations of As, capable of thriving in concentrations of up to 247,241 ppm of As(III) and 299,686 ppm of As(V).…”

Section: Recently Isolated Arsenic Tolerant Microorganisms and Approa...mentioning

confidence: 99%

Arsenic and Microorganisms: Genes, Molecular Mechanisms, and Recent Advances in Microbial Arsenic Bioremediation

William,

Magpantay

2023

Microorganisms

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Throughout history, cases of arsenic poisoning have been reported worldwide, and the highly toxic effects of arsenic to humans, plants, and animals are well documented. Continued anthropogenic activities related to arsenic contamination in soil and water, as well as its persistency and lethality, have allowed arsenic to remain a pollutant of high interest and concern. Constant scrutiny has eventually resulted in new and better techniques to mitigate it. Among these, microbial remediation has emerged as one of the most important due to its reliability, safety, and sustainability. Over the years, numerous microorganisms have been successfully shown to remove arsenic from various environmental matrices. This review provides an overview of the interactions between microorganisms and arsenic, the different mechanisms utilized by microorganisms to detoxify arsenic, as well as current trends in the field of microbial-based bioremediation of arsenic. While the potential of microbial bioremediation of arsenic is notable, further studies focusing on the field-scale applicability of this technology is warranted.

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“…from soda lake, mono lake, mine environment, and effluent plants ( B. arsenicoselenatis , B. selenitireducens , B. macyae and B. selenatarsenatis ) and As 3+ oxidation ( B. firmus L-148, Bacillus sp. PNKP-S2) have been described [ 36 , 37 , 46 – 49 ]. Metabolic versatility including the ability of dissimilatory reduction of Fe and As or even SO 4 by the members of this taxon could have strong influence of subsurface As mobility.…”

Section: Introductionmentioning

confidence: 99%

Molecular and taxonomic characterization of arsenic (As) transforming Bacillus sp. strain IIIJ3–1 isolated from As-contaminated groundwater of Brahmaputra river basin, India

et al. 2020

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Background: Microbe-mediated redox transformation of arsenic (As) leading to its mobilization has become a serious environmental concern in various subsurface ecosystems especially within the alluvial aquifers. However, detailed taxonomic and eco-physiological attributes of indigenous bacteria from As impacted aquifer of Brahmaputra river basin has remained under-studied. Results: A newly isolated As-resistant and-transforming facultative anaerobic bacterium IIIJ3-1 from Ascontaminated groundwater of Jorhat, Assam was characterized. Near complete 16S rRNA gene sequence affiliated the strain IIIJ3-1 to the genus Bacillus and phylogenetically placed within members of B. cereus sensu lato group with B. cereus ATCC 14579(T) as its closest relative with a low DNA-DNA relatedness (49.9%). Presence of iC17:0, iC15:0 fatty acids and menaquinone 7 corroborated its affiliation with B. cereus group, but differential hydroxy-fatty acids, C18:2 and menaquinones 5 & 6 marked its distinctiveness. High As resistance [Maximum Tolerable Concentration = 10 mM As 3+ , 350 mM As 5+ ], aerobic As 3+ (5 mM) oxidation, and near complete dissimilatory reduction of As 5+ (1 mM) within 15 h of growth designated its physiological novelty. Besides O 2 , cells were found to reduce As 5+ , Fe 3+ , SO 4 2− , NO 3 − , and Se 6+ as alternate terminal electron acceptors (TEAs), sustaining its anaerobic growth. Lactate was the preferred carbon source for anaerobic growth of the bacterium with As 5+ as TEA. Genes encoding As 5+ respiratory reductase (arr A), As 3+ oxidase (aioB), and As 3+ efflux systems (ars B, acr3) were detected. All these As homeostasis genes showed their close phylogenetic lineages to Bacillus spp. Reduction in cell size following As exposure exhibited the strain's morphological response to toxic As, while the formation of As-rich electron opaque dots as evident from SEM-EDX possibly indicated a sequestration based As resistance strategy of strain IIIJ3-1.

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Characterisation of hyper tolerant Bacillus firmus L-148 for arsenic oxidation

Cited by 32 publications

References 71 publications

Oligotrophy vs. copiotrophy in an alkaline and saline habitat of Lonar Lake

Oligotrophy vs. copiotrophy in an alkaline and saline habitat of Lonar Lake

Arsenic and Microorganisms: Genes, Molecular Mechanisms, and Recent Advances in Microbial Arsenic Bioremediation

Molecular and taxonomic characterization of arsenic (As) transforming Bacillus sp. strain IIIJ3–1 isolated from As-contaminated groundwater of Brahmaputra river basin, India

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