Characterisation of <i>Brucella</i> species and biovars in South Africa between 2008 and 2018 using laboratory diagnostic data

Matle, Itumeleng; Ledwaba, Betty; Madiba, None Karabelo; Makhado, Lavhelesani; Jambwa, Kudakwashe; Ntushelo, Nombasa

doi:10.1002/vms3.483

Cited by 18 publications

(13 citation statements)

References 43 publications

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“…A number of bacterial agents of abortion are contagious and/or become endemic in herds. Brucella abortus ( B. abortus ) is common and Brucella melitensis ( B. melitensis ) as well as Brucella ovis ( B. ovis ) are isolated sporadically in South Africa (Kolo et al 2019 ; Matle et al 2021 ; Mbizeni 2015 ). Campylobacter fetus ssp.…”

Section: Introductionmentioning

confidence: 99%

Approaches to increase recovery of bacterial and fungal abortion agents in domestic ruminants

Jonker

Thompson

Michel

2023

Onderstepoort Journal of Veterinary Research

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Abortions in domestic ruminants cause significant economic losses to farmers. Determining the cause of an abortion is important for control efforts, but it can be challenging. All available diagnostic methods in the bacteriology laboratory should be employed in every case due to the many limiting factors (autolysis, lack of history, range of samples) that complicate the investigation process. The purpose of this study was to determine whether the recovery of diagnostically significant isolates from domestic ruminant abortion cases could be increased through the use of a combination of the existing aerobic culture and Brucella selective method with methods that are commonly recommended in the literature reporting abortion investigations. These methods are examination of wet preparations and impression smears stained by the modified Ziehl–Neelsen method, anaerobic, microaerophilic, Leptospira, Mycoplasma and fungal culture. Samples of placenta and aborted foetuses from 135 routine clinical abortion cases of cattle (n = 88), sheep (n = 25) and goats (n = 22) were analysed by the new combination of methods. In 46 cases, bacteria were identified as aetiological agents and in one case a fungus. Isolation of Brucella species increased to 7.4% over two years compared with the previous 10 years (7.3%), as well as Campylobacter jejuni (n = 2) and Rhizopus species (n = 1). Salmonella species (5.9%) and Trueperella pyogenes (4.4%) were also isolated more often. In conclusion, the approach was effective in removing test selection bias in the bacteriology laboratory. The importance of performing an in-depth study on the products of abortion by means of an extensive, combination of conventional culture methods was emphasised by increased isolation of Brucella abortus and isolation of C. jejuni. The combination of methods that yielded the most clinically relevant isolates was aerobic, microaerophilic, Brucella and fungal cultures.

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Section: Introductionmentioning

confidence: 99%

Approaches to increase recovery of bacterial and fungal abortion agents in domestic ruminants

Jonker

Thompson

Michel

2023

Onderstepoort Journal of Veterinary Research

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“…The SAT is considered one of the most economical and, hence, widely used tests. The primary limitation of serological detection of brucellosis is the lack of discriminatory power to differentiate between Brucella species and biovars, as well as cross-reactivity with other gram-negative bacteria [ 11 ]. The added advantage of ELISA over SAT is that it can be used to test a larger number of samples, and the turnaround time is also less.…”

Section: Discussionmentioning

confidence: 99%

Brucella Seropositivity at a Tertiary Care Hospital in Chandigarh

Husain,

Sethi,

Yadav

2024

Cureus

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There are reports that the neglected zoonotic tropical disease brucellosis is reemerging today. Serological tests are being widely used in the diagnosis of brucellosis. In the present study, we performed a standard agglutination test (SAT) on 1348 suspected cases of brucellosis during the period of four years from April 2018 to March 2022. We noticed an increase in seropositivity from 2.6% in the year 2018-19 to 7.4% in the year 2021-22. We also noticed a spike in seropositivity in the years 2019-20 (12.5%). Our study shows the recent trend in seropositivity of the disease in Chandigarh and, hence, can be a meaningful addition to the existing serological diagnostic data related to brucellosis.

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“…MLVA11 identified B. abortus bv 3 isolated from cattle as new major clusters from WA. In contrast to WA where B. abortus biovar 3 strains occur in cattle, B. abortus biovar 1 is the dominate biovar in South Africa ( 46 ). These data suggest that the predominated B. abortus biovars are unique in WA compared to southern Africa, but further investigation is needed to better understand the epidemiological trait of B. abortus in Africa.…”

Section: Discussionmentioning

confidence: 99%

Original and introduced lineages co-driving the persistence of Brucella abortus circulating in West Africa

Liu

Wang

Shi

et al. 2023

Front. Public Health

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IntroductionBrucellosis, a serious public health issue affecting animals and humans, is neglected in West Africa (WA).MethodsIn the present study, bio-typing, multi-locus sequence typing (MLST), multiple-locus variable-number tandem repeat analysis (MLVA), and whole genome sequencing single-nucleotide polymorphism (WGS-SNP) analysis were used to characterize the Brucella abortus (B. abortus) strains from WA.ResultsAll of the 309 strains analyzed in this study were extracted and downloaded from the international MLVA bank and were from 10 hosts (cattle, humans, ovine, buffalo, dromedaries, horse, sheep, zebu, dog, and cat) distributed in 17 countries in WA. Based on the bio-typing, three biovars, dominated by B. abortus bv.3, were observed and reported across seven decades (1958–2019). With MLST, 129 B. abortus strains from the present study were sorted into 14 STs, with ST34 as the predicted founder. These 14 STs clustered into the global MLST data into three clone complexes (C I–C III) with the majority of strains clustering in C I, while C II forms an independent branch, and C III harbors three STs shared by different continents. These data revealed that most cases were caused by strains from native lineages. According to the MLVA-11 comparison, 309 strains were divided into 22 MLVA-11 genotypes, 15 of which were unique to WA and the remaining seven had a global distribution. MLVA-16 analysis showed that there were no epidemiological links among these strains. Based on the MLVA data, B. abortus strains from WA have high genetic diversity, and predominated genotypes were descended from a native lineage. While the MLVA-16 globally highlights that the dominant native and few introduced lineages (from Brazil, the USA, South Korea, Argentina, India, Italy, Portugal, the UK, Costa Rica, and China) co-driving the B. abortus ongoing prevalence in WA. The high-resolution SNP analysis implied the existence of introduced B. abortus lineages, which may be reasonably explained by the movement and trade of dominant hosts (cattle) and/or their products.DiscussionOur results indicated that B. abortus strains in WA consist of native and introduced strains that necessitate control such as vaccination, testing, slaughtering, and movement control by the relevant country authorities to reduce brucellosis in livestock.

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Characterisation of Brucella species and biovars in South Africa between 2008 and 2018 using laboratory diagnostic data

Abstract: The genus Brucella consists of pathogenic members that have zoonotic potential and are of veterinary and economic importance (Moreno, 2014). The pathogenic members of the genus Brucella cause a disease known as brucellosis in a wide variety of domes-

Cited by 18 publications

References 43 publications

Approaches to increase recovery of bacterial and fungal abortion agents in domestic ruminants

Approaches to increase recovery of bacterial and fungal abortion agents in domestic ruminants

Brucella Seropositivity at a Tertiary Care Hospital in Chandigarh

Original and introduced lineages co-driving the persistence of Brucella abortus circulating in West Africa

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