Characterisation of immunoglobulin-binding leucocytes in carp (Cyprinus carpio L.)

Diepen, JoséC.E. Koumans-van; Lisdonk, M.H.M. van de; Taverne-Thiele, Anja J.; Kemenade, B.M.L. Verburg-van; Rombout, J.H.W.M.

doi:10.1016/0145-305x(94)90251-8

Cited by 43 publications

(25 citation statements)

References 25 publications

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“…Humoral activities that have been shown to take place in both in vivo and in vitro assays include complement lysis of xenogeneic erythrocytes (Rijkers et al, 1980) and secretion of IgM-like immunoglobulins (Miller et al, 1985;Castillo et al, 1993;Koumans-Van Diepen et al, 1994b), interleukin-2 (Caspi & Avtalion, 1984) and macrophage-activating factor (Hardie et al, 1994).…”

Section: Introductionmentioning

confidence: 97%

Production and characterisation of a monoclonal antibody against the thymocytes of the sea bassDicentrarchus labrax(L.) (Teleostea, Percicthydae)

Scapigliati

Mazzini

Mastrolia

et al. 1995

Fish & Shellfish Immunology

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Section: Introductionmentioning

confidence: 97%

Production and characterisation of a monoclonal antibody against the thymocytes of the sea bassDicentrarchus labrax(L.) (Teleostea, Percicthydae)

Scapigliati

Mazzini

Mastrolia

et al. 1995

Fish & Shellfish Immunology

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“…PBLs, thymocytes, mid-kidney leukocytes, and HKLs were isolated as described previously (31,32). Head kidney-derived mature macrophages (referred to as macrophages) were obtained upon 6 d culture of HKLs, as previously described (33).…”

Section: Cell Isolation and Culturementioning

confidence: 99%

Carp Il10 Has Anti-Inflammatory Activities on Phagocytes, Promotes Proliferation of Memory T Cells, and Regulates B Cell Differentiation and Antibody Secretion

Piazzon

Savelkoul

Pietretti

et al. 2015

The Journal of Immunology

111

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In the current study, we investigated the effects of carp Il10 on phagocytes and lymphocytes. Carp Il10 shares several prototypical inhibitory activities on phagocytes with mammalian IL-10, including deactivation of neutrophils and macrophages, as shown by inhibition of oxygen and nitrogen radical production, as well as reduced expression of proinflammatory genes and mhc genes involved in Ag presentation. Similar to mammalian IL-10, carp Il10 acts through a signaling pathway involving phosphorylation of Stat3, ultimately leading to the early upregulation of socs3 expression. To our knowledge, this is the first study of the effects of Il10 on lymphocytes in fish. Although Il10 did not affect survival and proliferation of T cells from naive animals, it greatly promoted survival and proliferation of T cells in cultures from immunized animals, but only when used in combination with the immunizing Ag. Preliminary gene expression analysis suggests that, under these circumstances, carp Il10 stimulates a subset of CD8+ memory T cells while downregulating CD4+ memory Th1 and Th2 responses. In addition to the regulatory effect on T cells, carp Il10 stimulates proliferation, differentiation, and Ab secretion by IgM+ B cells. Overall, carp Il10 shares several prototypical activities with mammalian IL-10, including downregulation of the inflammatory response of phagocytes, stimulation of proliferation of subsets of memory T lymphocytes, and proliferation, differentiation, and Ab secretion by IgM+ B lymphocytes. To our knowledge, this is the first comprehensive analysis of biological activities of fish Il10 on both phagocytes and lymphocytes showing functional conservation of several properties of Il10.

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“…Peripheral blood leukocytes (PBL), mid-kidney leukocytes, head kidney leukocytes (HKL), and total thymocytes were isolated as described previously (14,15) and resuspended in the appropriate medium for the experiment as described below. Head kidney-derived mature macrophages (referred to as macrophages) were obtained upon 6 d culture of HKL as previously described (16).…”

Section: Cell Isolation and Culturementioning

confidence: 99%

Cyprinid Herpesvirus 3 Il10 Inhibits Inflammatory Activities of Carp Macrophages and Promotes Proliferation of Igm+ B Cells and Memory T Cells in a Manner Similar to Carp Il10

Piazzon

Wentzel

Tijhaar

et al. 2015

The Journal of Immunology

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Cyprinid herpesvirus 3 (CyHV-3) is the causative agent of a lethal disease of carp and encodes for an Il10 homolog (ORF134). Our previous studies with a recombinant ORF134-deleted strain and the derived revertant strain suggested that cyprinid herpesvirus 3 Il10 (CyHV-3 Il10 [cyhv3Il10]) is not essential for viral replication in vitro, or virulence in vivo. In apparent contrast, cyhv3Il10 is one of the most abundant proteins of the CyHV-3 secretome and is structurally very similar to carp Il10 and also human IL10. To date, studies addressing the biological activity of cyhv3Il10 on cells of its natural host have not been performed. To address the apparent contradiction between the presence of a structurally conserved Il10 homolog in the genome of CyHV-3 and the lack of a clear phenotype in vivo using recombinant cyhv3Il10-deleted viruses, we used an in vitro approach to investigate in detail whether cyhv3Il10 exerts any biological activity on carp cells. In this study, we provide direct evidence that cyhv3Il10 is biologically active and, similarly to carp Il10, signals via a conserved Stat3 pathway modulating immune cells of its natural host, carp. In vitro, cyhv3Il10 deactivates phagocytes with a prominent effect on macrophages, while also promoting proliferation of Igm+ B cells and memory T cells. Collectively, this study demonstrates a clear biological activity of cyhv3Il10 on cells of its natural host and indicates that cyhv3Il10 is a true viral ortholog of carp Il10. Furthermore, to our knowledge, this is the first report on biological activities of a nonmammalian viral Il10 homolog.

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Characterisation of immunoglobulin-binding leucocytes in carp (Cyprinus carpio L.)

Cited by 43 publications

References 25 publications

Production and characterisation of a monoclonal antibody against the thymocytes of the sea bassDicentrarchus labrax(L.) (Teleostea, Percicthydae)

Production and characterisation of a monoclonal antibody against the thymocytes of the sea bassDicentrarchus labrax(L.) (Teleostea, Percicthydae)

Carp Il10 Has Anti-Inflammatory Activities on Phagocytes, Promotes Proliferation of Memory T Cells, and Regulates B Cell Differentiation and Antibody Secretion

Cyprinid Herpesvirus 3 Il10 Inhibits Inflammatory Activities of Carp Macrophages and Promotes Proliferation of Igm+ B Cells and Memory T Cells in a Manner Similar to Carp Il10

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