Characterisation of microplastics and unicellular algae in seawater by targeting carbon via single particle and single cell ICP-MS

Vega, Raquel González de; Goyen, Samantha; Lockwood, Thomas E.; Doble, Philip; Camp, Emma F.; Clases, David

doi:10.1016/j.aca.2021.338737

Cited by 43 publications

(43 citation statements)

References 57 publications

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“…Some isolates, such as CCMP2464 had large variability in elementomes between biological replicates. A recent study advanced single-cell elementomic methods to allow quantification of the C content per cell in a Symbiodiniaceae culture, revealing significant heterogeneity within an isolate [ 54 ]. Whether this corresponds to associated bacteria [ 51 ], viruses [ 55 ] or may even represent elemental phenotypes within a given isolate requires further study.…”

Section: Discussionmentioning

confidence: 99%

Micronutrient content drives elementome variability amongst the Symbiodiniaceae

et al. 2022

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Background Elements are the basis of life on Earth, whereby organisms are essentially evolved chemical substances that dynamically interact with each other and their environment. Determining species elemental quotas (their elementome) is a key indicator for their success across environments with different resource availabilities. Elementomes remain undescribed for functionally diverse dinoflagellates within the family Symbiodiniaceae that includes coral endosymbionts. We used dry combustion and ICP-MS to assess whether Symbiodiniaceae (ten isolates spanning five genera Breviolum, Cladocopium, Durusdinium, Effrenium, Symbiodinium) maintained under long-term nutrient replete conditions have unique elementomes (six key macronutrients and nine micronutrients) that would reflect evolutionarily conserved preferential elemental acquisition. For three isolates we assessed how elevated temperature impacted their elementomes. Further, we tested whether Symbiodiniaceae conform to common stoichiometric hypotheses (e.g., the growth rate hypothesis) documented in other marine algae. This study considers whether Symbiodiniaceae isolates possess unique elementomes reflective of their natural ecologies, evolutionary histories, and resistance to environmental change. Results Symbiodiniaceae isolates maintained under long-term luxury uptake conditions, all exhibited highly divergent elementomes from one another, driven primarily by differential content of micronutrients. All N:P and C:P ratios were below the Redfield ratio values, whereas C:N was close to the Redfield value. Elevated temperature resulted in a more homogenised elementome across isolates. The Family-level elementome was (C19.8N2.6 P1.0S18.8K0.7Ca0.1) · 1000 (Fe55.7Mn5.6Sr2.3Zn0.8Ni0.5Se0.3Cu0.2Mo0.1V0.04) mmol Phosphorous-1 versus (C25.4N3.1P1.0S23.1K0.9Ca0.4) · 1000 (Fe66.7Mn6.3Sr7.2Zn0.8Ni0.4Se0.2Cu0.2Mo0.2V0.05) mmol Phosphorous -1 at 27.4 ± 0.4 °C and 30.7 ± 0.01 °C, respectively. Symbiodiniaceae isolates tested here conformed to some, but not all, stoichiometric principles. Conclusions Elementomes for Symbiodiniaceae diverge from those reported for other marine algae, primarily via lower C:N:P and different micronutrient expressions. Long-term maintenance of Symbiodiniaceae isolates in culture under common nutrient replete conditions suggests isolates have evolutionary conserved preferential uptake for certain elements that allows these unique elementomes to be identified. Micronutrient content (normalised to phosphorous) commonly increased in the Symbiodiniaceae isolates in response to elevated temperature, potentially indicating a common elemental signature to warming.

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Section: Discussionmentioning

confidence: 99%

Micronutrient content drives elementome variability amongst the Symbiodiniaceae

et al. 2022

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“…Another work by González de Vega et al 164 has explored the possibilities to use ICP-MS/MS in this context (see section 3.5. for a discussion on this approach 46,47 ). In this work, the authors recommend the monitoring of 12 C + instead of 13 Very recently, Liu et al 165 relied on the 13 C + monitoring to quantitatively analyze the microplastics ageing process (UV-light accelerated ageing dynamics) with a wide particle size range (800 nm to 5 m) and a PNC at an environmentally relevant value (down to 7.1 x 10 6 particles/L).…”

Section: Chemical Science Accepted Manuscriptmentioning

confidence: 99%

Living in a transient world: ICP-MS reinvented via time-resolved analysis for monitoring single events

et al. 2022

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“…Research fields and objectives. The research fields of the above references covered biology, 1-15 energy, 16 environment, 9,[17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32] food/medical, 10-12,25-27,33-40 geology, 28,41-47 material, [48][49][50][51][52][53][54][55][56][57][58][59][60] and radionulide, 4,[22][23][61][62][63][64][65][66][67][68][69][70][71] respectively. In addition to these conventional elemental analysis topics, a study on the gas phase catalytic reaction has also been reported by using the reaction cell of ICP-QMS/QMS as the in-situ reaction field.…”

Section: References Investigatedmentioning

confidence: 99%

“…73 Furthermore, ICP-QMS/QMS has been covered by principle studies and reviews on the ICP-MS reaction/collision cell technology, 72,74,75 gas chromatography (GC-) ICP-MS, 76 and high performance liquid chromatography (HPLC-) ICP-MS for quantitative profiling analysis of metalloids in biological samples, 77 respectively. ion-gas molecule reaction, 73 isotope, 3,4,22-24,26-28,43-47,62-66,68, single particle, 5,6,15,31,40,[54][55][56][57] and chemical speciation, 7,8,[10][11][12]16,76,77 respectively.…”

Section: References Investigatedmentioning

confidence: 99%

“…24 Single particle analysis. Single particle (or single cell) analysis based on ICP-QMS/QMS was reported for Ti, 5 (Nd, Eu, P), 6 (P, S, Fe, Cu, Zn, Pt), 15 C, 31 Fe, 40,[54][55][56][57] (Au, Ag, Pt), 56 and (Cr, Al, Ni, Cu, Au, Pt) 57 , respectively. Complementary software package for single particle analysis can facilitate the data processing, which can also be achieved by using spreadsheet software such as Microsoft Excel.…”

Section: Reaction Gases Used In the Measurement By Icp-qms/qmsmentioning

confidence: 99%

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Trends and Advances in Inductively Coupled Plasma Tandem Quadruple Mass Spectrometry (ICP-QMS/QMS) With Reaction Cell

Zhu¹

2021

At.Spectrosc.

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Trends and advances in the development and application of inductively coupled plasma tandem quadrupole mass spectrometry (ICP-QMS/QMS) with a reaction cell is reviewed mainly based on publications from January 2018 to July 2021. ICP-QMS/QMS has been applied in various research fields covering the sciences of biology, energy, environmental, food/medical, geology, materials, and radionuclide. The objectives of analysis cover the determination of elemental concentration, ion-gas reaction, isotope analysis, single particle analysis, and chemical speciation analysis. Measurement of most elements in the periodic table are reported except for H, N, O, F, rare gas, and some of the radionuclides. In addition to the default reaction/collision gases (i.e., He, H2, O2, and NH3), N2O, CO2, CH4, CH3F, C2H4, and C2H6 have been used as reaction gases to improve the capability of separating spectral interferences or to study the ion-molecule reactions. Typical applications of ICP-QMS/QMS analysis in the major research fields are also discussed.

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Characterisation of microplastics and unicellular algae in seawater by targeting carbon via single particle and single cell ICP-MS

Cited by 43 publications

References 57 publications

Micronutrient content drives elementome variability amongst the Symbiodiniaceae

Micronutrient content drives elementome variability amongst the Symbiodiniaceae

Living in a transient world: ICP-MS reinvented via time-resolved analysis for monitoring single events

Trends and Advances in Inductively Coupled Plasma Tandem Quadruple Mass Spectrometry (ICP-QMS/QMS) With Reaction Cell

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