Characterisation of Mytilus edulis hemocyte subpopulations by single cell time-lapse motility imaging

Foll, Frank Le; Rioult, Damien; Boussa, Sofiane; Pasquier, Jennifer; Dagher, Zeina; Leboulenger, F.

doi:10.1016/j.fsi.2009.11.011

Cited by 54 publications

(28 citation statements)

References 57 publications

(64 reference statements)

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“…The dispersion of velocities between slowest and fastest cells, enhanced at the peak of speed, may reflect the presence of distinct cell subpopulations. Given that (1) fluorescent nuclei were randomly selected for cell tracking and (2) hemocytes were capable of fast shape changes, we consider that these differences cannot be definitely attributed to the occurrence of previously characterized less-motile small round basophils and spread eosinophilic granulocytes (Le Foll et al 2010). Again, fast shape changes associated to hemocyte motion and cell to cell interactions should trigger caution when interpreting morphological variations as makers of effects (Magazine et al 1996;Hoher et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…We have previously shown that time-lapse videomicroscopy allows investigations on dynamics of mussel hemocytes shape changes and helps to propose a functional classification of cell subtypes (Le Foll et al 2010). In the present study, we describe a novel approach based on fluorescent staining and nuclei-tracking to determine migration velocity of hemocytes in vitro.…”

Section: Introductionmentioning

confidence: 91%

“…To control host-pathogen interactions and to respond immediately to invaders, mussels as others invertebrates, rely on innate immunity. Hemocytes found in hemolymph and infiltrating tissues, constitute a heterogeneous population of immune cells actively specialized in pleiotropic defences against pathogens (Carballal et al 1997;Donaghy and Volety 2011;Le Foll et al 2010). During the inflammatory response, activated hemocytes execute several major functions of cell-mediated innate immunity, including cytotoxic production of oxygen (Costa et al 2009;Winston et al 1996) or nitrogen species (Novas et al 2007), increased production of lysozymes ) and phagocytosis (Carballal et al 1997;Garcia-Garcia et al 2008;Le Foll et al 2010;Malagoli et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…In particular, whereas different types of hemocytes have been described so far (Cajaraville and Pal 1995;Friebel and Renwrantz 1995;Hine 1999;Le Foll et al 2010;Parisi et al 2008;Donaghy and Volety 2011), the origin, the role and the activation steps of hemocyte subpopulations are unknown. Furthermore, except for phagocytosis (GarciaGarcia et al 2008;Le Foll et al 2010;Voccia et al 1994), consensual protocols quantifying hemocyte activity or functional markers of inflammatory states are still lacking.…”

Section: Introductionmentioning

confidence: 99%

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Cell tracking and velocimetric parameters analysis as an approach to assess activity of mussel (Mytilus edulis) hemocytes in vitro

Rioult

Lebel²,

Foll

2013

Cytotechnology

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Hemocytes constitute the key element of innate immunity in bivalves, being responsible for secretion of antimicrobial peptides and release of zymogens from the prophenoloxidase system within the hemolymph compartment, reactive oxygen species production and phagocytosis. Hemocytes are found (and collected) as cells in suspension in circulating hemolymph. Hemocytes are adherent cells as well, infiltrating tissues and migrating to infected areas. In the present study, we applied an approach based on fluorescent staining and nuclei-tracking to determine migration velocity of hemocytes from the blue mussel, Mytilus edulis, in culture. Freshly collected hemocytes attached to substrate and start to move spontaneously in few minutes. Two main hemocyte morphologies can be observed: small star-shaped cells which were less motile and spread granular cells with faster migrations. Cell-tracking was combined to MTT mitochondria metabolic rate measurements in order to monitor global cell population activity over 4 days of culture. A transient peak of cell activity was recorded after 24-48 h of culture, corresponding to a speed up of cell migration. Videomicroscopy and cell tracking techniques provide new tools to characterize activity of mussel immunocytes in culture. Our analysis of hemocyte migration reveals that motility is very sensitive to cell environmental factors.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cell tracking and velocimetric parameters analysis as an approach to assess activity of mussel (Mytilus edulis) hemocytes in vitro

Rioult

Lebel²,

Foll

2013

Cytotechnology

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“…More sophisticated techniques, such labeling with monoclonal antibodies, revealed 3 groups: (i) basophilic granulocytes, (ii) basophilic granular and hyaline cells and (iii) eosinophilic granular cells [9]. Finally, single cell contrast microscopy time-lapse motility imaging combined with flow cytometry and cell volume determination revealed fast modifications of hemocyte aspect in vitro, rendering uncertain classification based on morphology [12]. As a consequence, classification and lineage are still in debate.…”

Section: Cytology Of Mytm-expressing Hemocytesmentioning

confidence: 99%

Individual variability of mytimycin gene expression in mussel

Cantet¹,

Toubiana²,

Parisi³

et al. 2012

Fish & Shellfish Immunology

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a b s t r a c tThe antifungal peptide mytimycin (MytM) is synthesized by hemocytes of the Mediterranean mussel, Mytilus galloprovincialis. In addition to sequence and gene structure diversities previously reported from pooled hemocytes, the present report focused on the expression of mytm gene in individual M. galloprovincialis, before and after challenge. Within untreated mussel, MytM mRNA was observed by ISH in about 42% of circulating hemocytes, characterized by large, diffuse nucleus. Injection with Fusarium oxysporum increased such percentage, but in only some of the mussels. Similarly, MytM gene expression increased after injection in only some of the mussels, as measured by qPCR. Responders and not responders are common evidence in any given population of organisms. Nevertheless, even if the use of proper pool size selection has been practised to find out and evaluate the most common response trends, individual analyses must be regarded as optimal.

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The Evolution of Adaptive Immunity

Danilova¹

2012

Advances in Experimental Medicine and Biology

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The concept of adaptive immunity suggests de novo generation in each individual of extremely large repertoires of diversified receptors and selective expansion of receptors that match the antigen/pathogen. Accordingly, adaptive immune system is also called "anticipatory". It allows each individual to have a unique repertoire of immune receptors corresponding to its life history. The memory of an antigen gets encoded in the clonal composition of the organism's immune cells instead of being encoded in the genome. Consequently, the immune response to repeated encounter with the same antigen becomes stronger, a phenomenon called immunological memory. Elements of adaptive immunity are found at all taxonomical levels, whereas in vertebrates, adaptive mechanisms have become the cornerstone of the immune system. In jaw vertebrates, adaptive immune receptors of T and B lymphoid cells belong to immunoglobulin superfamily and are created by rearrangement of gene segments. In jawless vertebrates lamprey and hagfish, recombination of leucine-rich repeat modules is used to form variable lymphocyte receptors. Striking functional similarity of the cellular and humoral branches of these systems suggests similar driving forces underlying their development.

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Characterisation of Mytilus edulis hemocyte subpopulations by single cell time-lapse motility imaging

Cited by 54 publications

References 57 publications

Cell tracking and velocimetric parameters analysis as an approach to assess activity of mussel (Mytilus edulis) hemocytes in vitro

Cell tracking and velocimetric parameters analysis as an approach to assess activity of mussel (Mytilus edulis) hemocytes in vitro

Individual variability of mytimycin gene expression in mussel

The Evolution of Adaptive Immunity

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