Characterisation of novel uveal melanoma cell lines under serum-free conditions

Suesskind, Daniela; Gauss, S.; Faust, Ulrike; Bauer, Peter; Schrader, Merle; Bartz-Schmidt, K. U.; Henke-Fahle, Sigrid

doi:10.1007/s00417-013-2292-9

Cited by 6 publications

(7 citation statements)

References 16 publications

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“…[44] A few cell lines, such as UPMM-1, UPMM-2, UMT33 have been shown to exhibit monosomy 3 and may serve as better models in future studies to examine the role of AMPs in uveal melanoma. [43, 45]…”

Section: Discussionmentioning

confidence: 99%

Expression of Antimicrobial Peptides by Uveal and Cutaneous Melanoma Cells and Investigation of Their Role in Tumor Cell Migration and Vasculogenic Mimicry

Manarang

Otteson

McDermott

2017

Current Eye Research

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Aims: Antimicrobial peptides (AMPs) have been implicated in the pathogenesis of several cancers, although there is also evidence suggesting potential for novel, AMP-based anti-tumour therapies. Discerning potential roles of AMPs in tumour pathogenesis may provide valuable insight on mechanisms of novel AMP-based anti-tumour therapy. Methods: mRNA expression of the AMPs α defensin (HNP-1); cathelicidin (LL-37); and β defensins (hBD-1, hBD-2, hBD-3, hBD-4) in human uveal and cutaneous melanoma cell lines, primary human uveal melanocytes, and primary human uveal melanoma cells was determined by RT-PCR. An in vitro scratch assay and custom Matlab analysis were used to determine AMP effects on melanoma cell migration. Lastly, the effect of specific AMPs on vasculogenic mimicry was determined by 3D culture and light and fluorescence microscopy. Results: Low to moderate, AMP transcript levels were detected and these varied across the cells tested. Overall, LL-37 expression was increased while hBD-4 was decreased in most melanoma cell lines, compared to primary cultured uveal melanocytes. There was no observable influence of HNP-1 and LL-37 on tumour cell migration. Additionally, aggressive cutaneous melanoma cells grown in 3D cultures exhibited vasculogenic mimicry, although AMP exposure did not alter this process. Conclusions: Collectively, our data show that although AMP mRNA expression is variable between uveal and cutaneous melanoma cells, these peptides have little influence on major characteristics that contribute to tumour aggressiveness and progression.

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Section: Discussionmentioning

confidence: 99%

Expression of Antimicrobial Peptides by Uveal and Cutaneous Melanoma Cells and Investigation of Their Role in Tumor Cell Migration and Vasculogenic Mimicry

Manarang

Otteson

McDermott

2017

Current Eye Research

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“…Another possibility might be the general inability of the tumour cells to metastasize. Proliferative activity of the UMT2 cells, their adhesion on different extracellular matrices and expression of numerous integrins have been assessed their invasive potential was not studied before. Regarding the genetic background, UMT2 is a cell line with proven disomy 3.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, our model uses human‐derived uveal melanoma cells (UMT2) that express many different melanocyte‐associated lineage markers and demonstrate a mutation in GNA11 , as do many uveal melanomas …”

Section: Discussionmentioning

confidence: 99%

“…UMT2 and UMT42 are primary cell lines that have been established in the Department of Ophthalmology, Tübingen. UMT2 has been characterized previously . Cell lines were cultivated in serum‐free Neurobasal A medium (Invitrogen® Life Technologies Corporation, Germany) supplemented with B27 Supplement (Invitrogen® Life Technologies Corporation, Germany, 1:50), L‐Glutamine 2 mM (PAA Laboratories GmbH, Austria), penicillin 100 U/mL (PAA Laboratories GmbH, Austria), streptomycin 100 µg/mL (PAA Laboratories GmbH, Austria), gentamycin 50 µg/mL (PAA Laboratories GmbH, Austria) and amphotericin B 2.5 µg/mL (PAA Laboratories GmbH, Austria) at 37 °C in a humidified incubator with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

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Novel mouse model for primary uveal melanoma: a pilot study

Süßkind

Hurst

Rohrbach

et al. 2016

Clinical Exper Ophthalmology

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“…1) or Chang D (Irvine Scientific), appears to improve the plating efficiency and the growth rate of the primary uveal melanoma cells [20]. More recently, Suesskind et al [21] have described the successful propagation of uveal melanoma cells in suspension using a serum-free neuronal medium (Neurobasal A; Invitrogen) supplemented with B27.…”

Section: Short-term Primary Cultures Of Uveal Melanoma Cells: Practicmentioning

confidence: 99%

Culturing Uveal Melanoma Cells

2015

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A major challenge in cancer research is the use of appropriate models with which to study a specific biological question. Cell lines have long been used to study cellular processes and the effects of individual molecules because they are easy to use, grow rapidly, produce reproducible results and have a strong track record in research. In uveal melanoma in particular, the absence of animal models that faithfully replicate the behavior of the human disease has propagated the generation and use of numerous cell lines by individual research groups. This in itself, however, can be viewed as a problem due to the lack of standardization when characterizing these entities to determine how closely they reflect the genetic and phenotypic characteristics of this disease. The alternative is to use in vitro primary cultures of cells obtained directly from uveal melanoma patient samples, but this too has its difficulties. Primary cell cultures are difficult to use, hard to obtain and can show considerable heterogeneity. In this article, we review the following: (1) the uveal melanoma cell lines that are currently available, discussing the importance of establishing a bank of those that represent the molecular heterogeneity of uveal melanoma; (2) the methods used to isolate and perform short-term cultures of primary uveal melanoma cells, and (3) the establishment of 3D tissue culture models that bridge the gap between 2D in vitro systems and in vivo models with which to dissect cancer biology and perform therapeutic screens.

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Characterisation of novel uveal melanoma cell lines under serum-free conditions

Cited by 6 publications

References 16 publications

Expression of Antimicrobial Peptides by Uveal and Cutaneous Melanoma Cells and Investigation of Their Role in Tumor Cell Migration and Vasculogenic Mimicry

Expression of Antimicrobial Peptides by Uveal and Cutaneous Melanoma Cells and Investigation of Their Role in Tumor Cell Migration and Vasculogenic Mimicry

Novel mouse model for primary uveal melanoma: a pilot study

Culturing Uveal Melanoma Cells

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