Characterisation of nucleosides and nucleobases in Mactra veneriformis by high performance liquid chromatography coupled with diode array detector-mass spectrometry (HPLC–DAD–MS)

Li, Rui; Ji, Jing; Wang, Lingchong; Shi-yong, Chen; Guo, Sheng; Wu, Hao

doi:10.1016/j.foodchem.2012.05.019

Cited by 28 publications

(27 citation statements)

References 19 publications

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“…Our previous study showed that M . veneriformis flesh samples contain polysaccharides, proteins, peptides, nucleosides, and fatty acids [13,14,15]; however, the peptide components have not been isolated or resolved. In the present study, we aimed to identify bioactive peptides that exhibit ACE inhibitory activity from the M .…”

Section: Introductionmentioning

confidence: 99%

Characterization of ACE Inhibitory Peptides from Mactra veneriformis Hydrolysate by Nano-Liquid Chromatography Electrospray Ionization Mass Spectrometry (Nano-LC-ESI-MS) and Molecular Docking

et al. 2014

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Food-derived bioactive compounds are gaining increasing significance in life sciences. In the present study, we identified angiotensin I-converting enzyme (ACE)-inhibitory peptides from Mactra veneriformis hydrolysate using a nano-LC-MS/MS method. Mactra veneriformis hydrolysate was first separated into four fractions (F1–F4) based on molecular weight by ultrafiltration. The fraction with molecular weight lower than 1 kDa (F1) showed the highest ACE inhibitory activity. F1 was then analyzed by a high throughput nano-LC-MS/MS method and sequences of peptides in F1 were calculated accordingly. The 27 peptides identified as above were chemically synthesized and tested for ACE-inhibitory activity. The hexapeptide VVCVPW showed the highest potency with an IC50 value of 4.07 μM. We then investigated the interaction mechanism between the six most potent peptides and ACE by molecular docking. Our docking results suggested that the ACE inhibitory peptides bind to ACE via interactions with His383, His387, and Glu411 residues. Particularly, similar to the thiol group of captopril, the cysteine thiol group of the most potent peptide VVCVPW may play a key role in the binding of this peptide to the ACE active site.

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Section: Introductionmentioning

confidence: 99%

Characterization of ACE Inhibitory Peptides from Mactra veneriformis Hydrolysate by Nano-Liquid Chromatography Electrospray Ionization Mass Spectrometry (Nano-LC-ESI-MS) and Molecular Docking

et al. 2014

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“…Nucleosides, nucleobases, and amino acids have been shown to exhibit various bioactivities, such as antioxidant, antiplatelet aggregation, antiarrhythmic, antiseizure, and anticancer effects, etc. [34][35][36][37] And, most of them are the quality control maker for medicinal herbs. [38][39][40] …”

Section: Structural Elucidationmentioning

confidence: 99%

Separation of polar antioxidants from Rhizoma Polygonatum Odorati by high-speed counter-current chromatography with a hydrophilic solvent system

Peng

Zhang

Shi

2016

Journal of Liquid Chromatography & Related Technologies

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Some highly polar compounds are quality control makers for medicinal herbs. However, investigation of them has been hampered because the existing fractionation steps are difficult and laborious to purify them. Similar situations happen to Rhizoma Polygonatum Odorati, a widely used food supplement and medicinal herb with strong antioxidant activity, and up to date, only ethyl acetate fraction of Rhizoma Polygonatum Odorati has been comprehensively investigated. Here, HSCCC using a hydrophilic solvent system composed of n-butanol-ethanol-2 M ammonium sulfate (1:1:2, v/v/v) was performed to isolate highly polar antioxidants in n-butanol fraction of Rhizoma Polygonatum Odorati, guided by DPPH-HPLC experiment. Afterward, Sephadex LH-20 column chromatography eluted by methanol was selected to eliminate ammonium sulfate and purify co-eluted compounds in HSCCC collected fractions. Finally, nine compounds, including four nucleosides, cytidine (1), uridine (4), guanosine (5), and adenosine (8); two nucleobases, guanine (3), and adenine (6); and three amino acids, tyrosine (2), phenylalanine (7), and tryptophan (9) with purities over 98% were achieved and identified by UV, MS, and 1 H NMR data. Notably, compounds 1-9 were first reported in genus Polygonatum. The results indicated that the proposed method was an efficient approach to isolate and purify highly polar compounds from complex extracts. GRAPHICAL ABSTRACT

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“…Therefore, it is a real challenge to analyze simultaneously nucleobases, nucleosides and ginsenosides in ginseng which also contains a myriad of other compounds with different polarities [15]. In addition, some LC methods involving multi-step gradients cannot be considered as fast approaches since the separation of ginsenosides often takes 35-60 min [16][17][18] and the separation of nucleosides and nucleobases requires 30-35 min [19,20], which limits their application in high-throughput analysis. Therefore, a fast separation method with good selectivity for nucleosides, nucleobases and ginsenosides would be of great interest for the quality control of ginseng products.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of nucleobases, nucleosides and ginsenosides in ginseng extracts using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

Huang

Zhang

Zhao

et al. 2017

Journal of Pharmaceutical and Biomedical Analysis

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Characterisation of nucleosides and nucleobases in Mactra veneriformis by high performance liquid chromatography coupled with diode array detector-mass spectrometry (HPLC–DAD–MS)

Cited by 28 publications

References 19 publications

Characterization of ACE Inhibitory Peptides from Mactra veneriformis Hydrolysate by Nano-Liquid Chromatography Electrospray Ionization Mass Spectrometry (Nano-LC-ESI-MS) and Molecular Docking

Characterization of ACE Inhibitory Peptides from Mactra veneriformis Hydrolysate by Nano-Liquid Chromatography Electrospray Ionization Mass Spectrometry (Nano-LC-ESI-MS) and Molecular Docking

Separation of polar antioxidants from Rhizoma Polygonatum Odorati by high-speed counter-current chromatography with a hydrophilic solvent system

Simultaneous analysis of nucleobases, nucleosides and ginsenosides in ginseng extracts using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

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