Characterisation of phenolic acid derivatives and flavonoids from different morphological parts of Helichrysum obconicum by a RP-HPLC–DAD-(−)–ESI-MSn method

Abstract

a b s t r a c tThe phenolic composition from different morphological parts of Helichrysum obconicum was investigated for the first time and 50 different phenolic compounds were detected. Phenolic acid conjugates, mainly mono-and di-caffeoylquinic acid derivatives, were the major components; some flavonoid derivatives were also detected in small amounts. Their separation and identification was performed by a high-performance liquid chromatography/electron spray ionisation tandem ion trap mass spectrometry method, with special emphasis on MS n fragmentation. The presence of di-and tricaffeoylshikimic acid isomers in Helichrysum species extracts was reported for the first time, the spectra of these compounds were mainly characterised by the presence of a [caffeoylshikimic acid-H] À ion at m/z 335. A lamiridosins-di-O-hexoside, an unusual component in Asteraceae species, was also detected.

Chemical composition of hydroethanolic extracts from five species of the Passiflora genus

“…The MS/MS spectrum on precursor ion at m/z 340.8 produced a fragment at m/z 178.7 (100%) (deprotonated caffeic acid) ( Table 1). The hexoside group probably was linked to caffeoyl moiety, since a base peak was observed at m/z 340.8 (Gouveia & Castilho, 2011;Negri et al, 2011). Compound 1 was characterized as a rosmarinic acid diglucoside, which was also found in bee pollen samples (Negri et al, 2011).…”

Anti-diabetic effects of Centratherum anthelminticum seeds methanolic fraction on pancreatic cells, β-TC6 and its alleviating role in type 2 diabetic rats

“…The fragmentation pattern of Compound B displays an ion peak at m/z 515, indicating the loss of the first caffeoyl, loss of the second caffeoyl at m/z 353, and loss of the third caffeoyl at m/z 191 to yield quinic acid, a loss of caffeic acid at m/z 179, and another caffeoyl at m/z 135. Thus, the compound was characterized as 3,4-O-dicaffeoylquinic acid (Lin and Harnly, 2008;Gouveia and Castilho, 2011). Compound C exhibits fragment ions at m/z 179 and m/z 135, the characteristic ions of caffeic acid (Luo et al, 2003;Lin and Harnly, 2008;Plazonic et al, 2011).…”

Performance Evaluation of Bidding-Based Multi-Agent Scheduling Algorithms for Manufacturing Systems

“…Samples rich in flavonoids are known to possess low LD50 values against Artemia salina [29,30]. Several flavonoids were identified in H. devium [13] and H. melaleucum [17], while H. obconicum was rich in quinic acid derivatives [18]. These differences in the composition can contribute to the different toxicity of each plant.…”

“…However, this hypothesis is excluded based on our recent studies [13,17,18] where the polar extracts of H. devium, melaleucum and obconicum were analyzed by HPLC-DAD-ESI/MS n and the major compounds identified were hydroxycinnamic acids, mainly quinic acid derivatives, and flavonoids normally in their O-glycosilated form, and no high molecular weight compounds were discovered. (H. obconicum, methanol) ( Table 1).…”