Honeydew honey, due to its higher
antibacterial and antioxidant
activity in comparison to blossom honeys, is in high demand and of
interest to consumers. Although a differentiation of blossom honeys
from honeydew honeys by way of electrical conductivity is given in
many cases, criteria for a differentiation of individual honeydew
honeys, such as spruce, fir, and pine, however did not exist. For
this reason, 93 authentic honeydew honeys and 63 non-honeydew honeys
[35 blossom and 28 nectar-honeydew (mixed)] from 13 different botanical
origins were collected within the framework of the current study,
and their electrical conductivity and phenolic and sugar profiles
were investigated. Results showed that the higher electrical conductivity
(≥0.80 mS/cm), the higher protocatechuic acid content (≥3.5
mg/kg), and the higher percentage of the oligosaccharide content (≥120
mg/g) were suitable parameters for the differentiation of authentic
coniferous honeydew honeys from non-honeydew honeys; a differentiation.
A differentiation of the spruce, fir, and pine honeydew honeys however
could not be reached. Through the analysis of 32 carbohydrates (2
mono-, 7 di-, 10 tri-, and 13 higher oligosaccharides) in only one
run by high-performance liquid chromatography equipped with an evaporative
light scattering detector, marker substances can now be utilized for
the classification of individual honeydew honeys. Sugar marker compounds
such as α,α-trehalose, melezitose, theanderose, nystose,
or maltotetraose in honeydew honeys in combination with chemometrics
highlighted the good capability of sugar profiles to discriminate
the honeydew honeys both from the non-honeydew honeys and from each
other. All in all, a 96.75% correct classification of all studied
156 honey samples was achieved by sugar marker compounds.