Characterisation of Plant Growth-Promoting Endophytic Bacteria from Sugarcane and Their Antagonistic Activity against Fusarium moniliforme

Pitiwittayakul, Nittaya; Wongsorn, Duanpen; Tanasupawat, Somboon

doi:10.21315/tlsr2021.32.3.6

Cited by 9 publications

(5 citation statements)

References 48 publications

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“…Table 2 ), while about one-half of the other MAGs cluster with established photosynthetic Acetobacteraceae such as Rhodopila (clades a, b, and LMUY01, Table 2 ). Conversely, two non-photosynthetic Antarctic MAGs (clade d, Table 2 ) cluster within the group of Lichenicoccus ( 36 ), which includes the recently reported ( 51 ) Acetobacteraceae KSS8 and KSS12 ( Fig. 1A and 2A ).…”

Section: Resultsmentioning

confidence: 89%

The phylogeny of Acetobacteraceae : photosynthetic traits and deranged respiratory enzymes

Degli Esposti,

Guerrero,

Rogel

et al. 2023

Microbiol Spectr

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We present here a comprehensive phylogenomic analysis of Acetobacteraceae , a vast group of alphaproteobacteria that has been widely studied for their economic importance. Our results indicate that the ancestor of Acetobacteraceae most likely was photosynthetic and evolved via a progressive transition from versatile photoferrotrophy to the incomplete oxidation of organic substrates defining acetous physiology. Vestigial signs of photosynthetic carotenoid metabolism are present in non-photosynthetic acetous taxa that have lost cytochrome oxidase, while their sister taxa retain such traits. The dominant terminal oxidase of acetous bacteria, the bo 3 ubiquinol oxidase, is derived from duplication and diversification of operons present in Acidocella taxa that have lost photosynthesis. We analyzed the bioenergetic traits that can compensate for the electron transfer function of photosynthetic reaction centers or constitute alternative pathways for the oxidoreduction of c -type cytochromes, such as iron oxidation. The latter pathway bypasses the deranged cytochrome bc 1 complex that is characteristically present in acidophilic taxa due to the loss of conserved ligands in both the Rieske iron-sulfur protein and cytochrome b subunit. The deranged or non-functional bc 1 complex may be retained for its structural role in stabilizing Complex I. The combination of our phylogenetic analysis with in-depth functional evaluations indicates that the order Acetobacterales needs to be emended to include three families: Acetobacteraceae sensu stricto , Roseomonadaceae fam. nov., and Acidocellaceae fam. nov. IMPORTANCE Acetobacteraceae are one of the best known and most extensively studied groups of bacteria, which nowadays encompasses a variety of taxa that are very different from the vinegar-producing species defining the family. Our paper presents the most detailed phylogeny of all current taxa classified as Acetobacteraceae , for which we propose a taxonomic revision. Several of such taxa inhabit some of the most extreme environments on the planet, from the deserts of Antarctica to the Sinai desert, as well as acidic niches in volcanic sites like the one we have been studying in Patagonia. Our work documents the progressive variation of the respiratory chain in early branching Acetobacteraceae into the different respiratory chains of acidophilic taxa such as Acidocella and acetous taxa such as Acetobacter . Remarkably, several genomes retain remnants of ancestral photosynthetic traits and functional bc 1 complexes. Thus, we propose that the common ancestor of Acetobacteraceae was photosynthetic.

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Section: Resultsmentioning

confidence: 89%

The phylogeny of Acetobacteraceae : photosynthetic traits and deranged respiratory enzymes

Degli Esposti,

Guerrero,

Rogel

et al. 2023

Microbiol Spectr

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“…The bacterial isolates were tested for their ability to inhibit the development of three phytopathogens, namely Lasiodiplodia sp., Pinicillium sp., and Aspergillus flavus. This was done using a dual-culture technique that measures the percentage of inhibition of radial growth (PIRG) [23]. Each phytopathogen was inoculated with a mycelial plug (5 mm) on one side of a Petri dish containing tryptic soy agar (TSA), positioned 20 mm away from the edge.…”

Section: Antifungal Activitiesmentioning

confidence: 99%

Some Direct and Indirect Mechanisms of Endophytic Bacteria That Associated with The Medicinal Plant Areva javanica from Shada Al-Asfal Mountain

Alzahrani,

Jastaniah,

Amasha

et al. 2024

Preprint

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Endophytes are ubiquitous organisms that reside within plant organs and contribute to the growth and well-being of plants. Nevertheless, there is limited knowledge regarding the plant growth-promoting endophytes found in medicinal plants. Therefore, the aims of the present work were to isolate some endophytic bacteria associated with roots, stems, leaves and inflorescences of Aerva javanica, collected from the Shada Alasfal Mountain at Albaha region, Saudi Arabia. After that, they were screened for some of their direct and indirect plant growth-promoting mechanisms. Finally, based upon their internal transcribed spacer 16S rRNA gene sequencing, the isolates were identified. The results showed that the selected endophytes differentially produced IAA and ammonia, and solubilized phosphate as direct mechanisms. In addition, they had exhibited variable capacities to produce some lytic enzymes and HCN beside their antifungal activities. Furthermore, they showed abilities to tolerate some stress conditions. The bacterial endophytes were identified by molecular methods and belonged to the genera of Micrococcus, Enterobacter, Pseudomonas, Delftia and Bacillus. The findings of this study indicated that the bacterial endophytes isolated from Aerva javanica are possessing crucial functions to improve plant growth and could be utilized as biofertilizers or bioagents to establish a sustainable crop production system.

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“…Endophytic bacteria spend at least one life cycle inside the tissues without inducing disease symptoms ( Pitiwittayakul et al., 2021 ). Endophytic microbial interactions exist only when a balance between the chemical reactions of colonizing bacteria and the host plant is achieved and maintained over time.…”

Section: Introductionmentioning

confidence: 99%

Molecular mechanism of endophytic bacteria DX120E regulating polyamine metabolism and promoting plant growth in sugarcane

Qin,

Khan,

Yan

et al. 2024

Front. Plant Sci.

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IntroductionSugarcane endophytic nitrogen-fixing bacterium Klebsiella variícola DX120E displayed broad impact on growth, but the exact biological mechanism, especially polyamines (PAs) role, is still meager.MethodsTo reveal this relationship, the content of polyamine oxidase (PAO), PAs, reactive oxygen species (ROS)-scavenging antioxidative enzymes, phytohormones, 1-aminocyclopropane-1-carboxylic synthase (ACS), chlorophyll content, and biomass were determined in sugarcane incubated with the DX120E strain. In addition, expression levels of the genes associated with polyamine metabolism were measured by transcriptomic analysis.ResultsGenomic analysis of Klebsiella variícola DX120E revealed that 39 genes were involved in polyamine metabolism, transport, and the strain secrete PAs in vitro. Following a 7-day inoculation period, DX120E stimulated an increase in the polyamine oxidase (PAO) enzyme in sugarcane leaves, however, the overall PAs content was reduced. At 15 days, the levels of PAs, ROS-scavenging antioxidative enzymes, and phytohormones showed an upward trend, especially spermidine (Spd), putrescine (Put), catalase (CAT), auxin (IAA), gibberellin (GA), and ACS showed a significant up-regulation. The GO and KEGG enrichment analysis found a total of 73 differentially expressed genes, involving in the cell wall (9), stimulus response (13), peroxidase activity (33), hormone (14) and polyamine metabolism (4).DiscussionThis study demonstrated that endophytic nitrogen-fixing bacteria stimulated polyamine metabolism and phytohormones production in sugarcane plant tissues, resulting in enhanced growth. Dual RNA-seq analyses provided insight into the early-stage interaction between sugarcane seedlings and endophytic bacteria at the transcriptional level. It showed how diverse metabolic processes selectively use distinct molecules to complete the cell functions under present circumstances.

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Characterisation of Plant Growth-Promoting Endophytic Bacteria from Sugarcane and Their Antagonistic Activity against Fusarium moniliforme

Cited by 9 publications

References 48 publications

The phylogeny of Acetobacteraceae : photosynthetic traits and deranged respiratory enzymes

The phylogeny of Acetobacteraceae : photosynthetic traits and deranged respiratory enzymes

Some Direct and Indirect Mechanisms of Endophytic Bacteria That Associated with The Medicinal Plant Areva javanica from Shada Al-Asfal Mountain

Molecular mechanism of endophytic bacteria DX120E regulating polyamine metabolism and promoting plant growth in sugarcane

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