Characterisation of plasma and tissue atrial natriuretic factor during development of moderate high output heart failure in the rat

Qing, Gefei; García, Raúl

doi:10.1093/cvr/27.3.464

Cited by 16 publications

(7 citation statements)

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“…After the initial rapid increase of atrial mRNA levels up to 21 d, there was a trend to decline from 28 d onwards. A reduction in plasma ANP levels after an early peak has been reported recently in AV fistula rats (53), and provides support for a correlation between myocardial mRNA prevalence and plasma ANP concentration, as found by others (e.g., 66,67).…”

Section: Discussionsupporting

confidence: 86%

“…It has less stringent ligand requirements than either NPR-A or NPR-B, binding ANP, BNP, and CNP with approximately equal affinity. In the AV fistula rats, circulating ANP concentrations are elevated (53) and are associated with a reduction in NPR-C mRNA in the heart. One might expect a clearance receptor to be turning over more rapidly when plasma ANP levels are high, and the prevalence of the mRNA for the receptor to rise pari passu.…”

Section: Discussionmentioning

confidence: 99%

“…A recent study suggested that the expression of mRNA transcripts for the receptors in the human heart may be modified by pressure or volume overload (51 ). In the present study, we have examined the temporal relationship between the expression of ANP and BNP, their receptors and increasing cardiac mass in a rat model of progressive four chamber cardiac hypertrophy and elevated plasma ANP levels, the aortovenocaval (AV) fistula rat (52,53). This has been accomplished using RT-PCR, as the technique provides a rapid approach to quantifying the expression of multiple genes in the same small cDNA sample.…”

Section: Introductionmentioning

confidence: 95%

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Differential regulation of natriuretic peptide receptor messenger RNAs during the development of cardiac hypertrophy in the rat.

Brown

Nunez²,

Wilkins³

1993

J. Clin. Invest.

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The heart expresses the three natriuretic peptide receptors (NPR), namely NPR-A, NPR-B, and NPR-C. We have examined the temporal relationship between the expression of mRNA transcripts for atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) and their receptors in the heart during the development of cardiac hypertrophy in the aortovenocaval fistula rat. Messenger RNAs were measured by cDNA amplification. Progressive cardiac hypertrophy was accompanied by increased ANP mRNA prevalence throughout the heart and increased BNP mRNA in the left atrium. The most striking observation was the gradual disappearance of NPR-C transcripts (the putative "clearance" receptor) in all chambers; this was in marked contrast to the increase in mRNA levels for NPR-A and NPR-B (the guanylyl cyclase-linked receptors). Our observations have important therapeutic implications if the transcript changes are mirrored at the receptor protein level because (a) the apparent down-regulation of NPR-C may enhance the local action of natriuretic peptides on the heart, and (b) the loss of NPR-C, particularly if it is widespread, may reduce the rate of elimination of the natriuretic peptides, restricting the therapeutic potential of specific NPR-C ligands designed to reduce peptide clearance. (J. Clin. Invest. 1993.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

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Differential regulation of natriuretic peptide receptor messenger RNAs during the development of cardiac hypertrophy in the rat.

Brown

Nunez²,

Wilkins³

1993

J. Clin. Invest.

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“…The ACF is sometimes regarded as a model for high‐output cardiac failure ( Garcia & Diebold 1990, Qing & Garcia 1992, Qing & Garcia 1993). However, dependent on the size of the fistula in relation of the size of the animal, the ACF procedure may result in anything from compensated cardiac hypertrophy to acute cardiac failure ( Legault et al .…”

Section: Discussionmentioning

confidence: 99%

Interaction between the renin–angiotensin system and insulin‐like growth factor I in aorto‐caval fistula‐induced cardiac hypertrophy in rats

Wåhlander

Wickman

Isgaard

et al. 1999

Acta Physiologica Scandinavica

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The effects of angiotensin converting enzyme inhibition and angiotensin II receptor blockade on the development of cardiac hypertrophy and myocardial insulin-like growth factor I (IGF-I) in volume overload were studied in male Wistar rats with aorto-caval fistulas (ACF). Rats were treated with ramipril (RAM, 3 mg kg(-1) day(-1)) for 4-20 days or losartan (LOS, 10 mg kg(-1) day(-1)) for 2-7 days. Myocardial IGF-I and IGF-I receptor (IGF-I-R) mRNA were determined by solution hybridization. ACF caused hypertrophy of left (LV) and right ventricles (RV). Hypertrophy appeared on day 2 and reached maximal values of +60% in LV and +75% in RV at day 12. Systolic blood pressure was initially reduced 15% but recovered by day 12. RAM abolished the recovery of blood pressure. Furthermore, RAM attenuated RV hypertrophy by 17% on day 7 and on day 20, RV weights were close to values found in controls. Beginning on day 9, RAM reduced LV weight back to control levels in parallel to blood pressure. In contrast, LOS affected neither RV nor LV hypertrophy. RV IGF-I mRNA increased 60-100% on day 7 alone in RV in ACF. RAM potentiated the increase in RV IGF-I to +400% and induced an increase in RV IGF-I-R mRNA on day 7 (+90%) in ACF. LOS did not affect RV IGF-I. Development of cardiac hypertrophy in ACF seemed independent of angiotensin II. RV hypertrophy was associated with activation of IGF-I independent of the renin-angiotensin system. IGF-I was further potentiated when development of hypertrophy was attenuated, possibly indicative of a greater urge for compensational growth in a relatively thinner and more volume-distended chamber.

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“…Aliquots of 50 p1 of the supernatant were acetylated for 3 min by adding 12.5 pl of a 1 :2 mix of acetic anhydride and triethylamine, followed by dilution (up to 500 pl) with sodium acetate buffer (pH 6.2). The cGMP content was then measured in 50-pl aliquots of these samples by radioimmunoassay, using an antibody raised at the Clinical Research Institute of Montreal and acetylated cGMP as a standard, as described previously (Qing and Garcia, 1993).…”

Section: Cyclic Gmp (Cgmp) Responses and Assaysmentioning

confidence: 99%

Effects of C‐Type Natriuretic Peptide on Rat Astrocytes: Regional Differences and Characterization of Receptors

Deschepper

Picard

1994

Journal of Neurochemistry

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We have compared the effects of atrial natriuretic peptide (ANP), brain natriuretic peptide (BMP), and C‐type natriuretic peptide (CNP) on the accumulation of cyclic GMP (cGMP) in secondary cultures of rat astro‐cytes. The order of potency of these peptides was CNP < ANP < BNP, which would be compatible with a predominance of guanylate cyclase B (GC‐B)‐versus guanylate cyclase A (GC‐A)‐type receptors in these cells. Accordingly, we found by northern blot analysis that the mRNA transcripts of GC‐B were much more abundant in astro‐cytes than the transcripts of GC‐A. In addition, astrocytes from diencephalon accumulated two times more cGMP in response to CNP than astrocytes from cortex. Binding experiments with 125l‐labeled ANP or [Tyr0]‐CNP established that these ligands recognized only clearance‐type receptors on astrocytes. However, the number of binding sites was ∼ 100 times higher in astrocytes from cortex than in astrocytes from diencephalon and thus was inversely correlated to the amplitude of the cGMP response in the same cells. We found no further evidence for differences in the levels of GC‐B receptors in astrocytes from the two regions because (a) the abundance of GC‐B mRNA was similar and (b) there was no difference in particulate guanylate cyclase activity in astrocytes from each region. In addition, occupancy of clearance receptors with C‐ANP4–23 did not affect the accumulation of cGMP in response to CNP; this makes it unlikely that the differences in cGMP responsiveness can be accounted for by binding and sequestration of CNP to the clearance receptors. Thus, the abundance of GC‐B receptors is not the only factor governing the amounts of cGMP they can generate on exposure to CNP. Other factors may regulate the ability of these receptors to generate cGMP in astrocytes.

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Characterisation of plasma and tissue atrial natriuretic factor during development of moderate high output heart failure in the rat

Cited by 16 publications

References 0 publications

Differential regulation of natriuretic peptide receptor messenger RNAs during the development of cardiac hypertrophy in the rat.

Differential regulation of natriuretic peptide receptor messenger RNAs during the development of cardiac hypertrophy in the rat.

Interaction between the renin–angiotensin system and insulin‐like growth factor I in aorto‐caval fistula‐induced cardiac hypertrophy in rats

Effects of C‐Type Natriuretic Peptide on Rat Astrocytes: Regional Differences and Characterization of Receptors

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