Characterisation of plasmids purified from Acetobacter pasteurianus 2374

Krahulec, Ján; Kretova, Miroslava; Grones, Jozef

doi:10.1016/j.bbrc.2003.08.125

Cited by 11 publications

(10 citation statements)

References 16 publications

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“…The G+C content of the pGP2 plasmid DNA is 54.1% what is less than that for the A. estunensis chromosomal DNA (58.7%) (Cleenwerck et al 2002). Similar observation with higher G+C content of chromosomal DNA in comparison with the plasmid DNA was reported for small plasmids pAG20 from A. aceti CCM (Czech Collection of Microorganisms) 3610 (G+C 51.6%) (Kretová et al 2005) and pAP2 from A. pasteurianus CCM 2374 (G+C 51%) (Krahulec et al 2003).…”

Section: Resultssupporting

confidence: 79%

“…Replication region of a large-scale bacterial plasmid contained Rep protein, binding sequence, iterons or repetitive sequences (Trček et al 2000;Krahulec et al 2003;Kretová et al 2005). Plasmid pGP2 encoded Rep protein essential for initiation of replication; replication region contains typical regulation-binding sites.…”

Section: Replication Modulementioning

confidence: 99%

“…The first identified cryptic plasmid from Acetobacter had been used for the construction of cloning vectors (Okumura et al 1985;Fukaya et al 1990;Tamaki et al 1991;Fujiwara et al 1992). Later a large plasmid pAC1 (19 kb) (Grones et al 1989;Grones & Turňa 1992), plasmid pAP12875 (Fomenkov et al 1995) and a large series of pAP1-pAP4 plasmids (Krahulec et al 2003) were indented from Acetobacter pasteurianus. Plasmid pJK2-1 (Trček et al 2000) and plasmid pAG20 (Kretová et al 2005) were isolated from Gluconobacter and Acetobacter aceti, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Nucleotide sequence analysis of small cryptic plasmid pGP2 from Acetobacter estunensis

Grones

2011

Biologia

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Complete nucleotide sequence of plasmid pGP2 from Acetobacter estunensis GP2 was identified after initial cloning of EcoRI fragment followed by preparation of deletion derivatives. Its size was defined to 2,797 bp and several sites for several restriction enzymes were revealed by DNA sequencing. Sequence analysis predicts three putative open reading frames (ORFs). ORF1 shows significant identity with the bacterial excinuclease α-subunit, ORF2 is a putative replication protein with low similarity with other Acetobacter plasmid's replication proteins, and ORF3 encodes a class B acid phosphatase/phosphotransferase. The replication module comprises a DnaA box like sequence, direct repeats, a potential prokaryotic promoter and a rep gene. The rep module is similar with several θ-replicating, iteron-containing modules from plasmids, suggesting pGP2 replication may follow the same course. Any phenotypic character determinant gene is absent in pGP2, suggesting this plasmid to be cryptic. However, a pGP2 derivative plasmid, containing the putative pGP2 rep region, can replicate and is stably maintained in Acetobacter and Escherichia coli strains; it can also carry foreign DNA fragments. Thus, pGP2-X could serve as a cloning shuttle vector between these bacteria. Prepared deletion derivatives of plasmid pGP2 suggested that Rep protein is essential for plasmid replication in host bacteria. In its natural host, A. estunensis GP2, pGP2 maintains a four-times lower copy number than in E. coli.

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Section: Resultssupporting

confidence: 79%

Section: Replication Modulementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nucleotide sequence analysis of small cryptic plasmid pGP2 from Acetobacter estunensis

Grones

2011

Biologia

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“…The identity of the region of pBR322 and pColE1 from E. coli, pAP2 from Acetobacter pasteurianus (Krahulec, 2003), pLG13 from E. coli, pPIGDM1 from Enterobacter agglomerans (Mikiewicz, 1997), p29807 from Yersinia entero- colitica (Strauch, 2000), pColJs from Shigella sonnei (Šmajs, 2001), pSFD10 from Salmonella choleraesuis (Liu, 2002) is 90, 92, 98, 95, 94, 91 and 92 %, respectively. The phylogenetic tree based on the homologous regions of the above-mentioned plasmids is shown in Figure 3.…”

Section: Resultsmentioning

confidence: 99%

“…pAH4 (Tonouchi et al 1994), pAP12875 (Fomenkov et al, 1995), pAP1 and pAP2 were isolated from Acetobacter pasteurianus (Krahulec et al, 2003), pJK2-1 from Acetobacter europaeus JK2 (Trček et al, 2000) and pAG20 from Acetobacter aceti 3620 (Kretová et al, 2005). Based on cryptic plasmid pAC1 from Acetobacter pasteurianus several plasmid vectors have been construed (Grones et al, 1991;Grones & Turňa, 1992).…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of plasmid pAP4 from Acetobacter pasteurianus

Grones

Kretova

2006

Biologia

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Abstract:The complete nucleotide sequence of plasmid pAP4 isolated from Acetobacter pasteurianus 2374T has been determined. Plasmid pAP4 was analysed and found to be 3,870 bp in size with a G+C content of 50.1%. Computer assisted analysis of sequence data revealed 2 possible ORFs with typical promoter regions. ORF1 codes for a protein responsible for kanamycin resistance similar with Tn5 transposone, ORF2 encodes a resistance to ampicillin identical with Tn3 transposone. Plasmid has in A. pasteurianus five copies and in E. coli DH1 about 30 copies per chromosome and it segregation stability in both strains is very high. Based on the data on replication region, plasmid does not code for a replication protein and origin region is similar with ColE1-like plasmid.

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Vinegar Fermentation

2006

Microbiology and Technology of Fermented Foods

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Characterisation of plasmids purified from Acetobacter pasteurianus 2374

Cited by 11 publications

References 16 publications

Nucleotide sequence analysis of small cryptic plasmid pGP2 from Acetobacter estunensis

Nucleotide sequence analysis of small cryptic plasmid pGP2 from Acetobacter estunensis

Molecular analysis of plasmid pAP4 from Acetobacter pasteurianus

Vinegar Fermentation

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