Characterisation Of Polysacharides And Lipids From Selected Green Algae Species By FTIR-ATR Spectroscopy

Bartošová, Alica; Blinová, Lenka; Gerulová, Kristína

doi:10.1515/rput-2015-0011

Cited by 14 publications

(10 citation statements)

References 10 publications

(7 reference statements)

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“…The vibration intensity, reported as absorbance, is proportional to the relative abundance of organic molecules such as carbohydrates, lipids, and proteins [48]. The FTIR spectra analysis of GS provides interesting information about the changes in the macromolecule composition induced by different growth conditions, confirming the usefulness of FTIR as a fast, non-disruptive method to identify macromolecules in microalgae [49,50]. The intensity of the peaks corresponding to proteins, lipids, and carbohydrates was greater in HGS than in AGS and Sp, with the only exception with the peak observed around 1540 cm −1 , which was higher in Sp and AGS, and was ascribable to N-H stretching of proteins.…”

Section: Infrared Spectrophotometrymentioning

confidence: 88%

Autotrophic and Heterotrophic Growth Conditions Modify Biomolecole Production in the Microalga Galdieria sulphuraria (Cyanidiophyceae, Rhodophyta)

et al. 2020

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Algae have multiple similarities with fungi, with both belonging to the Thallophyte, a polyphyletic group of non-mobile organisms grouped together on the basis of similar characteristics, but not sharing a common ancestor. The main difference between algae and fungi is noted in their metabolism. In fact, although algae have chlorophyll-bearing thalloids and are autotrophic organisms, fungi lack chlorophyll and are heterotrophic, not able to synthesize their own nutrients. However, our studies have shown that the extremophilic microalga Galderia sulphuraria (GS) can also grow very well in heterotrophic conditions like fungi. This study was carried out using several approaches such as scanning electron microscope (SEM), gas chromatography/mass spectrometry (GC/MS), and infrared spectrophotometry (ATR-FTIR). Results showed that the GS, strain ACUF 064, cultured in autotrophic (AGS) and heterotrophic (HGS) conditions, produced different biomolecules. In particular, when grown in HGS, the algae (i) was 30% larger, with an increase in carbon mass that was 20% greater than AGS; (ii) produced higher quantities of stearic acid, oleic acid, monounsaturated fatty acids (MUFAs), and ergosterol; (iii) produced lower quantities of fatty acid methyl esters (FAMEs) such as methyl palmytate, and methyl linoleate, saturated fatty acids (SFAs), and poyliunsaturated fatty acids (PUFAs). ATR-FTIR and principal component analysis (PCA) statistical analysis confirmed that the macromolecular content of HGS was significantly different from AGS. The ability to produce different macromolecules by changing the trophic conditions may represent an interesting strategy to induce microalgae to produce different biomolecules that can find applications in several fields such as food, feed, nutraceutical, or energy production.

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Section: Infrared Spectrophotometrymentioning

confidence: 88%

Autotrophic and Heterotrophic Growth Conditions Modify Biomolecole Production in the Microalga Galdieria sulphuraria (Cyanidiophyceae, Rhodophyta)

et al. 2020

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“…Infrared spectroscopy can identify the functional groups contained in a molecule (Zhang et al 2020). Dhivya et al (Dhivya 2017) have reported that the change of O-H may be related to the production of polyphenols and avonoids, and another study shows that phenolic compounds and avonoids are considered antioxidants and can be used as reducing agents and free radical scavengers (Gupta and Gupta 2011) Bartošová 2015) showed that 1644 cm − 1 is the characteristic band of protein spectrum. Yu et al (Yu et al 2020) analyzed infrared spectra and found that the absorbance of high concentration of Cd in the roots is greater than that of low concentration at the absorption band of 1631 cm − 1 -1637 cm − 1 .…”

Section: Discussionmentioning

confidence: 99%

Investigating Phragmites Australis Response to Copper Exposure Using Physiologic，FTIR and Metabolomic Approaches

Gao

et al. 2021

Preprint

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Background and aim Phragmites australis is a landscape plant with phytoremediation functions that is widely planted worldwide. However, little is known about the metabolomic background of the resistance mechanisms of Phragmites to heavy metals during its growth and development. Methods Here, we performed copper stress studies on Phragmites and monitored physiological indicators such as malondialdehyde (MDA) and electrolyte leakage (EL). In addition, FTIR was used to study chemical composition changes in the roots, stems and leaves of Phragmites seedlings under excessive copper stress. Furthermore, LC-MS technology combined with metabolomics data processing software was used to analyze the metabolic profile of samples.Result Copper contributed to the accumulation of MDA and EL. And the results of FTIR showed that the antioxidant effects of flavonoids and amino acids can be used by Phragmites leaf tissue to improve the tolerance of copper under 5 mg/L concentration. Further, the results of metabolomics reflected that Phragmites can improve its resistance to copper by increasing the accumulation of arginine and ayarin in the body. The former is accumulated through two pathways: the citrulline decomposition and conversion pathway and the circular pathway composed of ornithine, citrulline, L-Argininosuccinate and arginine. The latter is synthesized through the quercetin methylation pathway.Conclusion This study provides insights into the resistance mechanism and repair performance of Phragmites and other plant accumulators in response to copper stress.

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“…The peak at 1,645 cm À1 -1,623 cm À1 was assigned to C ¼ O stretching. The absorption band ranged from 1,404 cm À1 to 1,391 cm À1 could be assigned to C-O bending from carboxylate ions (Bartošová et al 2011). The band in the region from 1,100 cm À1 to 1,000 cm À1 in two samples might be characteristic of C-O stretching of carbohydrates (Zhao et al 2014).…”

Section: Ftir Analysismentioning

confidence: 94%

Influence of different iron sources on Sb(III) removal from water by active iron-oxidizing bacteria and its mechanism

Liu

et al. 2022

Water Science and Technology

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Iron-oxidizing bacteria played an important role in the treatment of Sb-containing wastewater. In this study, effect of different iron sources on Sb(III) removal ability by isolated iron-oxidizing bacteria (named as IOB-L) was conducted systemically in batch experiment. Moreover, ferrous lactate and zero-valent iron were chosen as iron sources for IOB-L. The results showed that after inoculation of 2% volume of IOB-L, Sb(III) concentration in water decreased from initial 18 mg/L to 4.1 mg/L at optimal pH of 7.0. There was no reaction between Sb(III) and ferrous lactate, whereas corrosion product of iron can adsorb certain amount of Sb. When active IOB-L cultivated in ferrous lactate, a better removal rate of Sb(III) can be reached with a longer stagnate phase for bacteria. However, Sb(III) removal ability of IOB-L using zero-valent iron as iron source was lower. SEM-EDS, FTIR, and XPS analysis further indicated that ferrous lactate was oxidized by IOB-L and precipitated as biogenic iron oxides which had strong adsorption ability towards Sb(III), whereas zero-valent iron was not a good iron source.

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Characterisation Of Polysacharides And Lipids From Selected Green Algae Species By FTIR-ATR Spectroscopy

Cited by 14 publications

References 10 publications

Autotrophic and Heterotrophic Growth Conditions Modify Biomolecole Production in the Microalga Galdieria sulphuraria (Cyanidiophyceae, Rhodophyta)

Autotrophic and Heterotrophic Growth Conditions Modify Biomolecole Production in the Microalga Galdieria sulphuraria (Cyanidiophyceae, Rhodophyta)

Investigating Phragmites Australis Response to Copper Exposure Using Physiologic，FTIR and Metabolomic Approaches

Influence of different iron sources on Sb(III) removal from water by active iron-oxidizing bacteria and its mechanism

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