Characterisation of pseudorabies virus in domestic pigs and wild boars in Croatia

Keros, Tomislav; Brnić, Dragan; Prpić, Jelena; Dežđek, Danko; Jemeršić, Lorena; Roić, Besi; Bedeković, Tomislav

doi:10.1556/avet.2014.020

Cited by 7 publications

(10 citation statements)

References 17 publications

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“…Similar findings were reported by Keros et al, [ 31 ]. The authors reported that genetically identical viral strains belonging to Clade A were circulating in Croatia in wild boar and domestic pigs, according to the gC sequences derived from six domestic pigs and one wild boar.…”

Section: Discussionsupporting

confidence: 92%

Evidence of Aujeszky’s disease in wild boar in Serbia

Milićević¹,

Radojičić²,

Valčić³

et al. 2016

BMC Vet Res

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BackgroundAujeszky’s disease is a viral disease of suids caused by Suid Herpesvirus 1. The disease has worldwide distribution with significant economic impact. In Serbia, there is neither an Aujeszky’s disease eradication nor national vaccination programme of domestic pigs.Since clinical symptoms of Aujeszky’s disease are not specific, it is important to establish a link between clinical signs and presence of ADV active infection in wild boars. The aim of this study was to investigate the possibility of active infection within wild boar showing signs of ADV and also to examine relationship between isolates from domestic pigs and wild boar. Having in mind that virus has not been previously isolated from wild boars in Serbia, we report the first isolation of Suid Herpesvirus 1 from this species in Serbia.ResultsTissue and serum samples from 40 wild boars from eastern Serbia were examined for evidence of Aujeszky’s disease (AD). Suid Herpesvirus 1 (SHV1), the cause of AD was isolated on PK15 cell line from three tissue samples, inducing cytopathic effect (CPE) with syncytia forming, and viral genome was detected by polymerase chain reaction (PCR) in eight samples. Genetic analysis of us4, us9 and ul49.5 partial sequences showed high homology between ADV isolates from wild boars and between isolates from wild boars and domestic animals. Neutralizing antibodies were not detected by virus neutralisation test (VNT) in sera from four out of eight PCR positive wild boars suggesting recent infection in those animals.ConclusionsThis is the first demonstration of Aujeszky’s disease virus (ADV) in the wild boar population in Serbia although seroconversion has been detected previously.

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Section: Discussionsupporting

confidence: 92%

Evidence of Aujeszky’s disease in wild boar in Serbia

Milićević¹,

Radojičić²,

Valčić³

et al. 2016

BMC Vet Res

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“…It is well known that PRV strains can spill over from the wild boar population to the domestic pig population and vice versa. Such bidirectional transmissions have been experimentally demonstrated [31,32], and phylogenetic analyses have confirmed that strains related to wild boars can cluster with those related to domestic pigs [11,33,34]. This is indeed the case here, with two French strains isolated from hunting dogs belonging to I-A Group 1 clustering with strains isolated from domestic pigs in other countries.…”

Section: Discussionsupporting

confidence: 78%

Genetic Diversity among Pseudorabies Viruses Isolated from Dogs in France from 2006 to 2018

et al. 2019

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Pseudorabies (PR), also known as Aujeszky’s disease, is an economically important disease for the pig industry. It has been eradicated in domestic pigs in many European countries, including France, but its causative agent—Suid Herpesvirus 1—is still circulating in wild boars. The risk of endemic PR in wild fauna lies in reintroducing the virus among domestic pigs and transmitting it to other mammals, especially hunting dogs for which the disease is rapidly fatal. As such infections are regularly reported in France, this study genetically characterized canine PR virus strains in the country to obtain information on their diversity and evolution. Partial sequencing of the glycoprotein C-encoding gene from 55 virus strains isolated from dogs between 2006 and 2018 showed that 14 strains belonged to genotype I-clade A and another 38 to genotype I-clade B, two clades usually reported in Western Europe. More surprisingly, three strains were found to belong to genotype II, suggesting an Asian origin. Genotype I-clade A strains exhibited the highest diversity as five geographically segregated genogroups were identified.

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“…The results of gC sequence comparison revealed high genetic similarity with previously derived strain from wild boars, but also domestic swine from Croatia (Keros and others 2014). …”

Section: Discussionmentioning

confidence: 58%

“…The results of all applied methods confirmed the presence of PrV in the brain tissue of both tested hunting dogs and defined it as the causative agent in both presented cases. Phylogenetic analysis revealed that both PrV strains derived from the brain tissue of infected dogs, clustered into a Clade A that comprises the majority of all characterised PrV strains along with PrV strains detected in domestic pigs and wild boars in Croatia (Keros and others 2014). PrV strains derived from dogs share 99.4 per cent and 99 per cent identities on nucleotide (nt) and amino acid (aa) level, respectively.…”

Section: Outcome and Follow‐upmentioning

confidence: 99%

Pseudorabies in hunting dogs in Croatia with phylogenetic analysis of detected strains

Keros

Jemeršić

Brnić

et al. 2015

Vet Record Case Reports

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Pseudorabies virus (PrV) can persist within the wild boar population allowing them to be potential reservoirs of PrV for domestic pigs but also other animal species including hunting dogs. This article presents a case report of PrV infection in two hunting dogs from different central Croatian districts. Clinically, symptoms regarding the CNS and automutilation predominated in both animals, following their death. Histological evidence of perivascular infiltration of brain tissue, positive cytopathological changes after inoculation into cell culture and PCR confirmed the presence of PrV. Further sequencing of PrV gC genome fragment confirmed the presence of Clade A genotype, similar to strains derived previously from domestic pigs and wild boars in Croatia. The first description of pseudorabies in hunting dogs in Croatia confirming the possibility of viral transmission through contact from wild boars to hunting dogs has been presented.

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Characterisation of pseudorabies virus in domestic pigs and wild boars in Croatia

Cited by 7 publications

References 17 publications

Evidence of Aujeszky’s disease in wild boar in Serbia

Evidence of Aujeszky’s disease in wild boar in Serbia

Genetic Diversity among Pseudorabies Viruses Isolated from Dogs in France from 2006 to 2018

Pseudorabies in hunting dogs in Croatia with phylogenetic analysis of detected strains

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