Characterisation of Pythium aristosporum Oomycete—A Novel Pathogen Causing Rice Seedling Blight in China

Liu, Jinxin; Zhang, Ruisi; Xu, Chuzhen; Liu, Chunlai; Zheng, Yanyan; Zhang, Xue; Li, Shasha; Li, Y. G.

doi:10.3390/jof8090890

Cited by 4 publications

(3 citation statements)

References 47 publications

(51 reference statements)

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“…Negative results were observed in 16 samples, particularly in lettuce and rice. These results may be attributed to the potential infection of the symptomatic seedlings by fungi or other oomycetes [47][48][49] or the possibility of inadequate detection of root samples. The former suggests the necessity of implementing a comprehensive plant disease diagnosis system that effectively encompasses all major pathogens of the plant.…”

Section: Discussionmentioning

confidence: 99%

Host Range and Loop-Mediated Isothermal Amplification Detection of Globisporangium sylvaticum from Guizhou, China

Zhang

Sun

et al. 2023

JoF

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Globisporangium, especially G. sylvaticum, causes devastating root rot, blight, and other diseases in various species of cash crops. To investigate the distribution and host range of G. sylvaticum in Guizhou, a suitable habitat for this pathogen, we collected 156 root-diseased samples, isolated the pathogens, and found that G. sylvaticum is widespread and has eleven host plants, including four novel hosts. Furthermore, to effectively identify G. sylvaticum, we developed a simple and dependable method based on loop-mediated isothermal amplification (LAMP), which used a primer set designed from the internal transcribed spacer sequences with high specificity and sensitivity of 1 pg/μL. Additionally, to perform field identification, we used the “Plant-LAMP” method with crude DNA extraction to detect the pathogen in 45 root samples from nine species of plants. Our results showed that this method could effectively detect G. sylvaticum in diseased roots. Therefore, our findings not only enrich existing research on the diversity of pathogenic Globisporangium in Guizhou but also present an efficient LAMP field detection method that could significantly contribute to plant disease management and prevention.

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Section: Discussionmentioning

confidence: 99%

Host Range and Loop-Mediated Isothermal Amplification Detection of Globisporangium sylvaticum from Guizhou, China

Zhang

Sun

et al. 2023

JoF

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“…[15], Burkholderia plantarii [16,17], Cochliobolus carbonum [18], Marasmius graminum [19], F. redolens [20], Enterobacter asburiae [21], Pantoea ananatis [21], Rhizoctonia solani [10], Fusarium spp. [10], Sclerotium rolfsii [10], Marasmius graminum [10], and Pythium aristosporum [22]. However, F. oxysporum remains the dominant pathogen causing RSB in the northeastern rice-planting areas of China [4,23].…”

Section: Introductionmentioning

confidence: 99%

Biological Control Ability and Antifungal Activities of Bacillus velezensis Bv S3 against Fusarium oxysporum That Causes Rice Seedling Blight

Jiang,

Liu,

et al. 2024

Agronomy

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Fusarium oxysporum, a primary soil-borne fungus that affects rice seedlings globally, is responsible for rice seedling blight (RSB), which reduces seedling quality and survival rates. The synthetic fungicides used to treat this disease negatively affect human health and the environment. A biocontrol bacterial isolate, Bacillus velezensis Bv S3, isolated from the rice rhizosphere, showed a strong antagonistic effect on RSB-causing F. oxysporum. The ratio of the longest to the shortest radius of F. oxysporum following Bv S3 inoculation was 2.52 (cm/cm) in a plate standoff experiment. This was different from the other biocontrol strains. Bv S3 exhibits a wide spectrum of antifungal activity against various pathogenic fungi that cause RSB. When 10% Bv S3 liquid culture filtrate was applied, it dramatically reduced F. oxysporum spore germination and mycelial growth, with inhibition rates of 66.7%, and 45.7%, respectively, and caused hyphal malformations. Furthermore, the Bv S3 suspension (1 × 108 CFU/mL) reduced RSB by 65.5% and 76.5% in pot experiments, effectively promoted the growth of rice seedlings, and improved the activities of neutral phosphatase, urease, invertase, and catalase in rice rhizosphere soil. The active substances produced by Bv S3 were sensitive to temperature and ultraviolet irradiation, and the antifungal effect significantly increased after 90 min of exposure, with antifungal effect observed at pH 7. Bv. S3 effectively reduced the incidence of RSB and showed potential as a biocontrol agent.

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“…Research has demonstrated that bakanae disease is caused by sever seed-borne Fusarium species, with Fusarium fujikuroi, which is a member of the Gibberella fujikuroi species complex, being the main contributor ( Amoah et al, 1995 ; Desjardins et al, 2000 ; Wulff et al, 2010 ). Seedling rot disease is caused by various seed-borne and soil-borne fungal pathogens, including Curvularia lunata , Fusarium oxysporum , and Rhizoctonia solani ( Verma et al, 2018 ; Song et al, 2021 ; Liu et al, 2022 ). Seedling rot disease influences the seeds and seedlings of rice plants, with discernible disease symptoms typically emerging shortly after seed sowing ( Limtong et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Development and application of a novel suspension concentrate for seed coating of rice for controlling bakanae disease and seedling rot disease

Ren,

Chen,

Yu-Chi

et al. 2024

Front. Bioeng. Biotechnol.

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The main rice planting areas in the middle and lower reaches of the Yangtze River are primarily affected by two types of rice seedling diseases: bakanae disease and seedling rot disease. These diseases lead to considerable losses. Seed coating technology effectively protects rice from these diseases and mitigates environmental pollution. We determined the antifungal activity of six fungicides, including phenamacril, azoxystrobin, fludioxonil, metconazole, thifluzamide and prothioconazole against Fusarium moniliforme Sheldon and Curvularia lunata in this study. In addition, the impact of fungicides and surfactants on rice seed germination were determined. Furthermore, phenamacril and fludioxonil were selected as the active components of suspension concentrate for seed coating. The antifungal activity of phenamacril against F. moniliforme Sheldon was 0.139 mg/L and fludioxonil against C. lunata was 0.110 mg/L. PEG-2000 was selected as the surfactant due to its promoting effect on rice seedling. Based on the above findings, 6% phenamacril fludioxonil FS was developed via the wet sand grinding method. The toxicity of 6% phenamacril fludioxonil FS to zebrafish was verified, and field experiments were conducted in five different regions of the Yangtze River Basin. The results indicated minimal toxicity of 6% phenamacril fludioxonil FS to zebrafish. Relative to the control agent consisting of 6.25% phenamacril metalaxyl-M FS, 6% phenamacril fludioxonil FS showed better control effect and exhibited superior efficacy in promoting growth and increasing yield in all five regions. Specifically, the control effect of 6% phenamacril fludioxonil FS on bakanae exceeded 84.83% with the highest yield increasing value recorded at 30.48%. Currently, the market offers a limited selection of suspension concentrate for seed coating of rice. The findings of this study may offer a viable alternative formulation and directions for further research concerning the application of suspension concentrate for seed coating of rice.

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Characterisation of Pythium aristosporum Oomycete—A Novel Pathogen Causing Rice Seedling Blight in China

Cited by 4 publications

References 47 publications

Host Range and Loop-Mediated Isothermal Amplification Detection of Globisporangium sylvaticum from Guizhou, China

Host Range and Loop-Mediated Isothermal Amplification Detection of Globisporangium sylvaticum from Guizhou, China

Biological Control Ability and Antifungal Activities of Bacillus velezensis Bv S3 against Fusarium oxysporum That Causes Rice Seedling Blight

Development and application of a novel suspension concentrate for seed coating of rice for controlling bakanae disease and seedling rot disease

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