Characterisation of QoI‐resistant field isolates of Botrytis cinerea from citrus and strawberry

Ishii, Hideo; Fountaine, J. M.; Chung, Wen‐Hsin; Kansako, Masanori; Nishimura, Kumiko; Takahashi, K.; Oshima, Michiyo

doi:10.1002/ps.1773

Cited by 86 publications

(80 citation statements)

References 25 publications

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“…Previous studies indicated that alternative oxidase (AOX) is one way to decrease the damage of strobilurin fungicides in certain fungal pathogens. 33,41,42) Furthermore, the culture medium added with an AOX inhibitor of salicylhydroxamic acid (SHAM) or n-propyl gallate (PG) could increase the inhibitory activity of strobilurins against fungal pathogens. 33,41,42) In this study, the M. oryzae isolates showed variable sensitivity to azoxystrobin and kresoxim-methyl, and the different sensitivities might be caused by the level of AOX activity in each M. oryzae isolate.…”

Section: Discussionmentioning

confidence: 99%

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Phylogenetic diversity and sensitivity to MBI and QoI fungicides of Magnaporthe oryzae in Taiwan

Hsieh

Chung²,

Chen³

2013

J. Pestic. Sci.

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Magnaporthe oryzae (Anamorph: Pyricularia oryzae), the causal agent of rice blast disease, is the major pathogen that reduces the yield of rice worldwide. Melanin biosynthesis inhibitors (MBIs) and strobilurins (QoIs) are common fungicides used to control the disease. One hundred and three M. oryzae isolates from different rice-production areas in Taiwan were evaluated for their molecular phylogeny and sensitivity to MBI and QoI fungicides. Molecular phylogenetic analysis showed that all M. oryzae isolates could be classified into nine genetic groups (A-I) based on the DNA polymorphism amplified by CNS1/MP primers. Predominant ones are groups A (72 isolates) and B (15 isolates). Fungicide tests showed that M. oryzae isolates were sensitive to MBIs (carpropamid and tricyclazole) and less sensitive to QoIs (kresoxim-methyl and azoxystrobin). Amplifying the cytochrome b (cyt b) gene revealed that isolates with low sensitivity to QoIs did not correspond with mutation at codon 129 or 143 in the cyt b gene.

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Section: Discussionmentioning

confidence: 99%

“…ABC transports are also closely related to multiple drug resistances. 21,42) Thus, the AOX and ABC transports might play an important role in reducing the damage of strobilurin fungicides in the less sensitive M. oryzae isolates from Taiwan. It warrants further investigation to confirm the mechanism of the resistance to strobilurins.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic diversity and sensitivity to MBI and QoI fungicides of Magnaporthe oryzae in Taiwan

Hsieh

Chung²,

Chen³

2013

J. Pestic. Sci.

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“…Specific resistance has been described e.g. to benzimidazoles such as benomyl, thiophanate-methyl and carbendazim (Yarden & Katan, 1993;Yourman & Jeffers, 1999), dicarboximides such as iprodione and vinclozolin (Northover & Matteoni, 1986;Yourman & Jeffers, 1999), QoI fungicides (strobilurins; Bardas et al, 2010;Ishii et al, 2009), anilinopyrimidines such as cyprodinil and pyrimethanil (Chapeland et al, 1999;Myresiotis et al, 2007), carboxamides/SDHIs (boscalid; Leroux et al, 2010), and the hydroxyanilide compound fenhexamid (Fillinger et al, 2008;Ziogas et al, 2003). To the best of our knowledge, no reports of specific resistance to the phenylpyrrole compound fludioxonil have been published as yet.…”

Section: Fungicide Resistance In B Cinereamentioning

confidence: 99%

Multiple Fungicide Resistance in Botrytis: A Growing Problem in German Soft-Fruit Production

Weber¹,

Entrop²

2011

Fungicides - Beneficial and Harmful Aspects

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“…Yoshi-7, Yoshi-14 and Yoshi-20 were collected from citrus plants in Wakayama Prefecture, Japan. 11,12) M09149, M09152, M09161, M09163, M09182 and M09212 were collected from tomatoes in Mie Prefecture, Japan. The sensitive strains KU-26-1, KU-59 and KU-76, maintained in the Life Science Research Institute of Kumiai, were used as the standard.…”

Section: Reference Isolatesmentioning

confidence: 99%

“…11) These isolates change from glycine to alanine at position 143 (G143A) in the cytochrome b gene. 12) On the other hand, inhibitory effects of pyribencarb to cytochrome b enzyme on the transfer system of a QoI-resistant strain of B. cinerea were stronger than those of other QoI fungicides. 4) In other words, the binding site of pyribencarb to cytochrome b was assumed to be a little different from that of other QoI fungicides.…”

Section: Introductionmentioning

confidence: 97%

A method for monitoring the sensitivity of Botrytis cinerea to pyribencarb

et al. 2011

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Pyribencarb is a novel benzylcarbamate-type fungicide having excellent activity against a wide range of plant pathogenic fungi, especially gray mold diseases caused by Botrytis cinerea. The primary target site of pyribencarb is the Qo site of cytochrome b in the electron transfer system of the respiratory chain. The inhibitory effects of pyribencarb to cytochrome b enzyme on the transfer system of a QoI-resistant strain of B. cinerea are stronger than those of other QoI fungicides. Pyribencarb exhibited adequate control in greenhouse trials when QoI-resistant strains were inoculated. We have developed a practical method for evaluating the sensitivity of B. cinerea to pyribencarb. The final concentrations of pyribencarb in the PDA medium were set at 1 and 100 mg a.i. per liter with 1 mM salicylhydroxamic acid (SHAM). The isolates that were completely inhibited at 1 mg a.i. per liter of pyribencarb were "sensitive strains," and the isolates that were inhibited by more than 80% at 100 mg a.i. per liter of pyribencarb were "weakly resistant strains." In our assays so far, no strains with mycelial growth inhibition rates of less than 80% at 100 mg per liter of pyribencarb have ever been found. In the event that such strains should be found in the future, it will be necessary to monitor their sensitivity to pyribencarb using an in vivo assay.

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Characterisation of QoI‐resistant field isolates of Botrytis cinerea from citrus and strawberry

Abstract: The conventional RFLP and sequence analyses of PCR-amplified cytochrome b gene are insufficient for molecular identification of QoI resistance in B. cinerea.

Cited by 86 publications

References 25 publications

Phylogenetic diversity and sensitivity to MBI and QoI fungicides of <i>Magnaporthe oryzae</i> in Taiwan

Phylogenetic diversity and sensitivity to MBI and QoI fungicides of <i>Magnaporthe oryzae</i> in Taiwan

Multiple Fungicide Resistance in Botrytis: A Growing Problem in German Soft-Fruit Production

A method for monitoring the sensitivity of Botrytis cinerea to pyribencarb

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