Characterisation of rat and human tissue alkaline phosphatase isoforms by high-performance liquid chromatography and agarose gel electrophoresis

Intestinal alkaline phosphatase: multiple biological roles in maintenance of intestinal homeostasis and modulation by diet

“…This information is required to more clearly delineate the respective roles of IAP and other AP isoforms. Combining chromatography, electrophoresis, enzymology, and immunochemistry will help resolve this complexity 102–104 …”

Evaluation of serum bone alkaline phosphatase activity in patients with liver disease: Comparison between electrophoresis and chemiluminescent enzyme immunoassay

“…Bone ALP is an extracellular enzyme that is found anchored to the osteoblast membrane and which reflects whole skeletal remodeling [11]; it is one of the most commonly used biochemical markers for osteoblastic bone formation analyzed in the routine clinical chemistry laboratory [12,13]. Metastatic bone tumors, diabetes, hyperthyroidism, fracture recovery period and other conditions lead to the hyperfunction of bone metabolism or formation, which, in turn, increases serum bone ALP levels.…”

Biochemical and proteomic investigation of bovine nasal secretion

“…Alkaline phosphatase has been studied and characterized using several techniques in many species including cattle (Healy, 1971;Antonov et al, 1983;Van Hoof et al, 1988;Horney et al, 1992;Itoh et al, 2002 andDziedziejko et al, 2009). In 1933, Graham and Kay initially demonstrated that AP activity in bovine milk was heat labile and that AP measurements could be used to monitor the effectiveness of pasteurization where AP is inactivated at a temperature that is slightly higher than normal pasteurization conditions at 65°C (Chaudhuri et al, 2013).…”