Characterisation of recombinant immunoreactive antigens of the scab mite Sarcoptes scabiei

Kühn, Cornelia; Lucius, Richard; Matthes, H.-F.; Meusel, G.; Reich, Björn; Kalinna, Bernd H.

doi:10.1016/j.vetpar.2008.02.007

Cited by 24 publications

(18 citation statements)

References 41 publications

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“…The cloning of SM proteins corresponding to those of the predominant HDM allergens has now facilitated studies of their sequence homology (25,26,34,45,46,49,50), localization (47,51), enzymatic activity (7,16,22,35,40), and interactions with the immune system (8,11,30). Here, we have studied differences in the types and magnitudes of the systemic antibody and cellular responses to characterize the specific immune response in OS and compare it to the more severe and debilitating crusted form of the disease (CS).…”

Section: Discussionmentioning

confidence: 99%

Increased Allergic Immune Response toSarcoptes scabieiAntigens in Crusted versus Ordinary Scabies

Walton

Pizzutto

Slender

et al. 2010

Clin Vaccine Immunol

122

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Scabies, a parasitic skin infestation by the burrowing "itch" mite Sarcoptes scabiei, causes significant health problems for children and adults worldwide. Crusted scabies is a particularly severe form of scabies in which mites multiply into the millions, causing extensive skin crusting. The symptoms and signs of scabies suggest host immunity to the scabies mite, but the specific resistant response in humans remains largely uncharacterized. We used 4 scabies mite recombinant proteins with sequence homology to extensively studied house dust mite allergens to investigate a differential immune response between ordinary scabies and the debilitating crusted form of the disease. Subjects with either disease form showed serum IgE against recombinant S. scabiei cysteine and serine proteases and apolipoprotein, whereas naive subjects showed minimal IgE reactivity. Significantly (P < 0.05) greater serum IgE and IgG4 binding to mite apolipoprotein occurred in subjects with crusted scabies than in those with ordinary scabies. Both subject groups showed strong proliferative responses (peripheral blood mononuclear cells) to the scabies antigens, but the crusted scabies group showed increased secretion of the Th2 cytokines interleukin 5 (IL-5) and IL-13 and decreased Th1 cytokine gamma interferon (IFN-␥) in response to the active cysteine protease. These data confirm that a nonprotective allergic response occurs in the crusted disease form and demonstrate that clinical severity is associated with differences in the type and magnitude of the antibody and cellular responses to scabies proteins. A quantitative IgE inhibition assay identified IgE immunoreactivity of scabies mite antigens distinct from that of house dust mite antigens, which is potentially important for specific scabies diagnosis and therapy.

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Section: Discussionmentioning

confidence: 99%

Increased Allergic Immune Response toSarcoptes scabieiAntigens in Crusted versus Ordinary Scabies

Walton

Pizzutto

Slender

et al. 2010

Clin Vaccine Immunol

122

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“…Other ELISAs have been used for the diagnosis of mange in rabbits (Arlian et al, 1994) however, to the best of our knowledge they have not been characterized regarding their cut off level, sensitivity, and specificity. Other S. scabiei recombinant antigens with repetitive sequences (similar to Ss20 B3), which have been suggested to be involved in immune evasion, have been assayed for their suitability for diagnosis (in porcine and human sera), giving a high non-specific background reaction of the negative sera (Kuhn et al, 2008). Zhang et al (2013) have developed a dot-ELISA assay for the serodiagnosis of sarcoptic mange in rabbits using recombinant thioredoxin peroxidase with high sensitivity (95.3%) and specificity (93.8%) representing a rapid, convenient, and efficient technique.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of an ELISA using recombinant Ssλ20ΔB3 antigen for the serological diagnosis of Sarcoptes scabiei infestation in domestic and wild rabbits

Casáis

Millán

Rosell³

et al. 2015

Veterinary Parasitology

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“…Arthropods that can pierce vertebrate skin have mouthparts especially adapted for piercing, cutting or burrowing (e.g. Reid 2002;Kuhn et al 2008). The deer ked, as other louse flies, is adapted for piercing skin of the host with feeding apparatus including three sets of tiny teeth, one set situated just in the entrance of haustellum (Haarløv 1964).…”

Section: Discussionmentioning

confidence: 99%

New bedding site examination-based method to analyse deer ked (Lipoptena cervi) infection in cervids

et al. 2008

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Invasion of the deer ked (Lipoptena cervi), an ectoparasitic fly commonly found in cervids, has been rapid in Finland during the last four decades. As the distribution area of this species has expanded from the south towards the northern latitudes, the associated problems have become more evident. Various animals such as horses, cattle and especially reindeer have been reported to host this parasite. Moreover, in certain areas, the deer ked causes major inconveniences for humans potentially limiting recreational activities in forests. We wanted to study if deer ked parasitism and intensity of the infection in winter time could be detected by using visual examination of the snow on cervid bedding sites and by analysing biotic samples found from the bedding sites. Our results demonstrate that chronic deer ked infection causes reddish-brown snow discolouration (host tissue fluid and deer ked faeces) on the bedding sites to the extent that parasitism can be diagnosed. Hence, we suggest that deer ked infection prevalence and range expansion could be rapidly monitored using our new practical and reliable method. In the future, bedding site analyses will likely be useful in predicting and potentially preventing the negative effects of this ecologically and socio-economically important parasite.

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Characterisation of recombinant immunoreactive antigens of the scab mite Sarcoptes scabiei

Cited by 24 publications

References 41 publications

Increased Allergic Immune Response toSarcoptes scabieiAntigens in Crusted versus Ordinary Scabies

Increased Allergic Immune Response toSarcoptes scabieiAntigens in Crusted versus Ordinary Scabies

Evaluation of an ELISA using recombinant Ssλ20ΔB3 antigen for the serological diagnosis of Sarcoptes scabiei infestation in domestic and wild rabbits

New bedding site examination-based method to analyse deer ked (Lipoptena cervi) infection in cervids

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