Characterisation of six α-expansin genes in Gossypium hirsutum (upland cotton)

Se, Harmer; Sj, Orford; Jn, Timmis

doi:10.1007/s00438-002-0721-2

Cited by 94 publications

(61 citation statements)

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“…Down-regulated was also observed in the mutant cotton at fiber elongation stage (5–15 dpa), and cellulose synthases and sucrose synthase secondary cell wall biosynthesis stage (15–20 dpa). The following physiological and biochemical processes were also involved in fiber development: phytohormones such as ethylene (Shi et al, 2006), auxins (Yang et al, 2006; Zhang et al, 2011) and brassinosteroids (Sun et al, 2005; Luo et al, 2007); transcription factors such as MYB25 (Machado et al, 2009) and MYB25-like (Guan et al, 2011; Walford et al, 2011); osmotically active solutes such as soluble sugars, potassium and malate, ion-transporters such as H + -ATPases and K + -transporter that is important in maintaining the osmotic potential of the elongating fiber cell (Wang and Ruan, 2010); closure of plasmodesmata and the coordinated up-regulation of K+ and sugar transporters during fiber elongation to maintain the turgor pressure for the fiber cell elongation and the duration of plasmodesmata closure (Ruan et al, 2004); carbohydrate and energy metabolisms and their role in providing the carbon skeletons for cell wall polysaccharides and fatty acids synthesis (Gou et al, 2007; Pang et al, 2008, 2010a,b; Yang et al, 2008); the involvement of xyloglucan and pectin enzymes (Lee et al, 2010), arabinogalactans (Liu et al, 2008), and expansins (Harmer et al, 2002) in cell wall loosening and expansion during fiber elongation; secondary cell wall involvement during fiber elongation (Li et al, 2002, 2005; Wang et al, 2010); reactive oxygen species (ROS) homeostasis and its involvement in initiation and differentiation (Liu et al, 2012). …”

Section: Introductionmentioning

confidence: 99%

Effects of fuzzless cottonseed phenotype on cottonseed nutrient composition in near isogenic cotton (Gossypium hirsutum L.) mutant lines under well-watered and water stress conditions1

Bellaloui

Turley

2013

Front. Plant Sci.

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There is no information available on the effect of fuzzless seed trait on cottonseed nutrient composition (minerals, N, S, protein, and oil) under drought stress. The objective of this research was to investigate the effect of the fuzzless seed trait on cottonseed nutrients using five sets of near-isogenic lines (NILs). Each set consists of two lines that share the same genetic background, but differ in seed fuzziness (fuzzy, F; fuzzless, N). The near isogenic lines will enable us to compare the effect of the trait without confounding the genotypic background effects. We hypothesized that since the fuzzless trait involved in fiber initiation development, and was reported to be involved in biochemical, molecular, and genetic processes, this trait may also alter cottonseed nutrient composition. Results showed that NIL sets accumulated different levels of minerals in seeds and leaves, and the fuzzless trait (N) in most of the lines altered seed and leaf mineral accumulations when compared with fuzzy lines (F) or the control line. For example, K, P, Mg, Cu, and Na concentrations in seeds were higher in MD N and STV N than in their equivalent MD F and STV F lines. Leaf concentrations of Ca, K, Mg, S, B, Cu, and Fe in MD N lines were higher than MD F line. Lower levels of nutrients in seeds and leaves were observed under water stress conditions, especially Ca, Mg, N, and B in seeds.Generally and with few exceptions, seed protein was higher in fuzzy lines than in fuzzless lines; however, seed oil was higher in fuzzless lines than in fuzzy lines. Our research demonstrated that fuzzless trait altered the composition and level of nutrients in seed and leaves in well watered and water stressed plants. Differences in protein and oil between fuzzy and fuzzless seeds may indicate alteration in nitrogen and carbon fixation and metabolism. The differential accumulation of seed nutrients in this germplasm could be used by cotton breeders to select for higher cottonseed quality.

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Section: Introductionmentioning

confidence: 99%

Effects of fuzzless cottonseed phenotype on cottonseed nutrient composition in near isogenic cotton (Gossypium hirsutum L.) mutant lines under well-watered and water stress conditions1

Bellaloui

Turley

2013

Front. Plant Sci.

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“…The high expression of these transporter genes facilitates the uptake of sucrose and potassium, hence, the generation of the observed low osmotic potential (Ruan et al 2001). The closure of the fibre PD likely enables the estimated high turgor to be maintained to drive the elongation as long as osmotically active solutes are continuously imported to attract water (Cosgrove 1997;Pfluger and Zambryski 2001) and the cell wall is expandable through the expression of expansin genes (Ruan et al 2000;Harmer et al 2002). Consistent with this hypothesis is the reopening of fibre PD, which correlates with the decrease of estimated fibre turgor and the termination of the elongation process (Ruan et al 2001).…”

Section: The Power Of Symplastic Isolation In Fibre Elongationmentioning

confidence: 99%

Rapid cell expansion and cellulose synthesis regulated by plasmodesmata and sugar: insights from the single-celled cotton fibre

Ruan¹

2007

Functional Plant Biol.

103

102

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Abstract.Higher plants comprise mixtures of some 40 different cell types, and this often complicates the interpretation of data obtained at the tissue level. Studies for a given cell type may provide novel insights into the mechanisms underlying defined cellular and developmental processes. In this regard, the cotton fibre represents an excellent single-cell model to study the control of rapid cell elongation and cellulose synthesis. These single cells, initiated from the ovule epidermis at anthesis, typically elongate to ∼3-5 cm in the tetraploid species before they switch to intensive secondary cell wall cellulose synthesis. By maturity, more than 94% of fibre weight is cellulose. To unravel the mechanisms of fibre elongation and cellulose synthesis, two hypotheses have been examined: (a) that sucrose degradation and utilisation mediated by sucrose synthase (Sus) may play roles in fibre development and (b) that symplastic isolation of the fibre cells may be required for their rapid elongation. Reverse genetic and biochemical analyses have revealed the critical role that Sus plays in fibre initiation and early elongation. Late in development, plasma-membrane and cell wall association of Sus protein seems to be involved in rapid cellulose synthesis. Cell biology and gene expression studies showed a temporary closure of fibre plasmodesmata (PD), probably due to the deposition of callose, at the rapid phase of elongation. The duration of the PD closure correlates positively with the final fibre length attained. These data support the view that PD closure may be required for fibres to achieve extended elongation. The branching of PD towards the secondary cell wall stage is postulated to function as a molecule sieve for tight control of macromolecule trafficking into fibres to sustain intensive cellulose synthesis.

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“…During rapid fiber elongation, it is known that genes encoding wall-loosening expansin proteins [74,75] are expressed and are associated with quantitative trait loci linked with fiber length [72,76,77]. In this study, expansins were also found to be differentially regulated.…”

Section: Discussionmentioning

confidence: 63%

Comparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibers

et al. 2016

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The distinct stages of cotton fiber development and maturation serve as a single-celled model for studying the molecular mechanisms of plant cell elongation, cell wall development and cellulose biosynthesis. However, this model system of plant cell development is compromised for proteomic studies due to a lack of an efficient protein extraction method during the later stages of fiber development, because of a recalcitrant cell wall and the presence of abundant phenolic compounds. Here, we compared the quality and quantities of proteins extracted from 25 dpa (days post anthesis) fiber with multiple protein extraction methods and present a comprehensive quantitative proteomic study of fiber development from 10 dpa to 25 dpa. Comparative analysis using a label-free quantification method revealed 287 differentially-expressed proteins in the 10 dpa to 25 dpa fiber developmental period. Proteins involved in cell wall metabolism and regulation, cytoskeleton development and carbohydrate metabolism among other functional categories in four fiber developmental stages were identified. Our studies provide protocols for protein extraction from maturing fiber tissues for mass spectrometry analysis and expand knowledge of the proteomic profile of cotton fiber development.

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Characterisation of six α-expansin genes in Gossypium hirsutum (upland cotton)

Cited by 94 publications

References 50 publications

Effects of fuzzless cottonseed phenotype on cottonseed nutrient composition in near isogenic cotton (Gossypium hirsutum L.) mutant lines under well-watered and water stress conditions1

Effects of fuzzless cottonseed phenotype on cottonseed nutrient composition in near isogenic cotton (Gossypium hirsutum L.) mutant lines under well-watered and water stress conditions1

Rapid cell expansion and cellulose synthesis regulated by plasmodesmata and sugar: insights from the single-celled cotton fibre

Comparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibers

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