2012
DOI: 10.1016/j.colsurfb.2011.09.007
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Characterisation of spin coated engineered Escherichia coli biofilms using atomic force microscopy

Abstract: The ability of biofilms to withstand chemical and physical extremes gives them the potential to be developed as robust biocatalysts. Critical to this issue is their capacity to withstand the physical environment within a bioreactor; in order to assess this capability knowledge of their surface properties and adhesive strength is required. Novel atomic force microscopy experiments conducted under growth conditions (30 o C) were used to characterise Escherichia coli biofilms, which were generated by a recently d… Show more

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Cited by 18 publications
(12 citation statements)
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“…Deposition and maturation determine the structure of the biofilm and thus the mass transfer of chemical species through the biofilm extracellular matrix, therefore defining its overall performance as a biocatalyst (Tsoligkas et al, 2011; 2012). We have recently developed methods to generate engineered biofilms, utilising centrifugation of recombinant E. coli onto poly- l -lysine coated glass supports instead of waiting for natural attachment to occur (Tsoligkas et al, 2011; 2012).…”
Section: Introductionmentioning
confidence: 99%
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“…Deposition and maturation determine the structure of the biofilm and thus the mass transfer of chemical species through the biofilm extracellular matrix, therefore defining its overall performance as a biocatalyst (Tsoligkas et al, 2011; 2012). We have recently developed methods to generate engineered biofilms, utilising centrifugation of recombinant E. coli onto poly- l -lysine coated glass supports instead of waiting for natural attachment to occur (Tsoligkas et al, 2011; 2012).…”
Section: Introductionmentioning
confidence: 99%
“…Deposition and maturation determine the structure of the biofilm and thus the mass transfer of chemical species through the biofilm extracellular matrix, therefore defining its overall performance as a biocatalyst (Tsoligkas et al, 2011; 2012). We have recently developed methods to generate engineered biofilms, utilising centrifugation of recombinant E. coli onto poly- l -lysine coated glass supports instead of waiting for natural attachment to occur (Tsoligkas et al, 2011; 2012). These biofilms were used to catalyse the biotransformation of 5-haloindole plus serine to 5-halotryptophan (Figure 1a), an important class of pharmaceutical intermediates; this reaction is catalysed by a recombinant tryptophan synthase TrpBA expressed constitutively from plasmid pSTB7 (Tsoligkas et al, 2011; 2012; Kawasaki et al 1987).…”
Section: Introductionmentioning
confidence: 99%
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“…Figure S11). Recently, the higher adhesive force values was suggested to be associated with 442 stronger polyprotein stretching at the surface EPS of E. coli biofilms (Tsoligkas et al 2012). …”
mentioning
confidence: 99%
“…EPS has been confirmed as a major mechanism controlling biofilm adhesion [59][60][61][62]. As a result, a number of studies have been undertaken to assess the effect of growth conditions, chemical treatments, and novel antimicrobials on the production of EPS and the reduction in adhesion.…”
Section: Adhesion Studiesmentioning
confidence: 99%