Characterisation of strains of Salmonella serotype Livingstone by multiple typing

Crichton, Pamela B.; Old, D. C.; Taylor, Adam; Rankin, Shelley C.

doi:10.1099/00222615-44-5-325

Cited by 19 publications

(23 citation statements)

References 18 publications

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“…However, the results of both approaches (PFGE and ribotyping) for the epidemiological evaluation of the isolates involved in this outbreak suggested that it was caused by the same clone. Salmonella serotype Livingstone has been isolated previously from poultry in other countries: Canada (11), Scotland (11,35), Japan (28), England (11), and France (13). Between 1980 and 1985 in Scotland, poultry and poultry products were an important vehicle for human salmonellosis, since poultry meat accounts for Ͼ50% of all human Salmonella outbreaks where a food vehicle has been identified (35).…”

Section: Discussionmentioning

confidence: 99%

Nosocomial Outbreak Caused by Salmonella enterica Serotype Livingstone Producing CTX-M-27 Extended-Spectrum β-Lactamase in a Neonatal Unit in Sousse, Tunisia

Bouallègue-Godet¹,

Salem²,

Fabre

et al. 2005

J Clin Microbiol

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In this study, we report an outbreak of Salmonella enterica serotype Livingstone resistant to extendedspectrum cephalosporins that occurred in a neonatal ward of the maternity department of Farhat Hached Hospital, Sousse, Tunisia, in 2002. A total of 16 isolates were recovered from 16 babies hospitalized in the ward during the period 1 to 16 July. All these babies developed diarrhea, and three of them developed septicemia. All the isolates demonstrated resistance to ceftriaxone and ceftazidime due to the production of an extendedspectrum ␤-lactamase (ESBL). The isolates were also resistant to aminoglycosides (kanamycin, tobramycin, netilmicin, gentamicin, and amikacin) and sulfamethoxazole-trimethoprim. DNA profiles were determined by pulsed-field gel electrophoresis using the XbaI and SpeI endonucleases and by ribotyping with PstI digestion. They yielded the same patterns, showing that the outbreak was caused by a single clone. The ESBL was identified as CTX-M-27 by sequencing of PCR products and by isoelectric focusing. The ESBL resistance was transferred by a 40-kb conjugative plasmid. The mobile insertion sequence ISEcp1 was found to be located upstream of bla CTX-M-27 in the same position as that known for a bla CTX-M-14 sequence. A new gene named dfrA21, encoding resistance to trimethoprim and carried by a 90-kb plasmid, was characterized. The dfrA21 gene was inserted as a single resistance cassette in a class I integron. The babies were treated with colistin, and all except two recovered. The outbreak came to an end when appropriate actions were taken: patient isolation, hand washing, and disinfection of the ward.Salmonellosis in humans is usually a self-limited gastroenteritis that does not warrant antimicrobial therapy. However, these infections can occasionally lead to life-threatening systemic infections that require effective chemotherapy. With the emergence of ampicillin-resistant Salmonella enterica serotype Typhimurium DT104 isolates, extended-spectrum cephalosporins (ESC) are currently the agents of choice for such chemotherapy, especially for children, for whom the use of fluroquinolones is not yet approved. The selective pressure created by the use of ESC has been described as one of the most important factors in the appearance of extended-spectrum

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Section: Discussionmentioning

confidence: 99%

Nosocomial Outbreak Caused by Salmonella enterica Serotype Livingstone Producing CTX-M-27 Extended-Spectrum β-Lactamase in a Neonatal Unit in Sousse, Tunisia

Bouallègue-Godet¹,

Salem²,

Fabre

et al. 2005

J Clin Microbiol

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“…The present study, in agreement with previous experiments (11), found that the vast majority of serovar Enteritidis strains carry just the serospecific virulence plasmid. Extrachromosomal DNA is regarded as an unstable genetic marker (2,5). Also, it is recognized that strains with the same chromosomal features may show different plasmid restriction patterns (28) and that the same plasmid profile may be present in strains which are different at a chromosomal level (24).…”

Section: Figmentioning

confidence: 99%

Diversity of Strains of Salmonella enterica Serotype Enteritidis from English Poultry Farms Assessed by Multiple Genetic Fingerprinting

et al. 2001

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Reliable and sufficiently discriminative methods are needed for differentiating individual strains of Salmonella enterica serotype Enteritidis beyond the phenotypic level; however, a consensus has not been reached as to which molecular method is best suited for this purpose. In addition, data are lacking on the molecular fingerprinting of serotype Enteritidis from poultry environments in the United Kingdom. This study evaluated the combined use of classical methods (phage typing) with three well-established molecular methods (ribotyping, macrorestriction analysis of genomic DNA, and plasmid profiling) in the assessment of diversity within 104 isolates of serotype Enteritidis from eight unaffiliated poultry farms in England. The most sensitive technique for identifying polymorphism was PstI-SphI ribotyping, distinguishing a total of 22 patterns, 10 of which were found among phage type 4 isolates. Pulsed-field gel electrophoresis of XbaI-digested genomic DNA segregated the isolates into only six types with minor differences between them. In addition, 14 plasmid profiles were found among this population. When all of the typing methods were combined, 54 types of strains were differentiated, and most of the poultry farms presented a variety of strains, which suggests that serotype Enteritidis organisms representing different genomic groups are circulating in England. In conclusion, geographical and animal origins of Salmonella serotype Enteritidis isolates may have a considerable influence on selecting the best typing strategy for individual programs, and a single method cannot be relied on for discriminating between strains.

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“…It also provided a good level of discrimination for serotype Senftenberg. Previous studies have found plasmid profiling to be of use in intraserotype differentiation (6,20,24), although many of these were performed prior to the widespread implementation of the more advanced methods of PFGE and ribotyping. Few recently published articles involve the use of plasmid profiling as a stand-alone procedure.…”

Section: Fig 1 Dendrogram Generated By the Gel Compar II Software Smentioning

confidence: 99%

“…This is a limitation which must be recognized in epidemiological research and has brought into question what can be regarded as a suitable plasmid profile for analysis. Some studies (6) regard the presence of a single, identical plasmid as sufficient proof that isolates are identical and therefore epidemiologically related. Other studies (15) suggest that numerous plasmids must be present and regard the presence of a single plasmid insufficient as representative of a clone.…”

Section: Fig 1 Dendrogram Generated By the Gel Compar II Software Smentioning

confidence: 99%

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Molecular Typing of Salmonella Serotypes Prevalent in Animals in England: Assessment of Methodology

Liébana¹,

Guns

Migura-García³

et al. 2001

J Clin Microbiol

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Salmonella enterica serotypes Derby, Mbandaka, Montevideo, Livingstone, and Senftenberg were among the 10 most prevalent serotypes isolated from farm animals in England and Wales in 1999. These serotypes are of potential zoonotic relevance; however, there is currently no "gold standard" fingerprinting method for them. A collection of isolates representing the former serotypes and serotype Gold Coast were analyzed using plasmid profiling, pulsed-field gel electrophoresis (PFGE), and ribotyping. The success of the molecular methods in identifying DNA polymorphisms was different for each serotype. Plasmid profiling was particularly useful for serotype Derby isolates, and it also provided a good level of discrimination for serotype Senftenberg. For most serotypes, we observed a number of nontypeable plasmid-free strains, which represents a limitation of this technique. Fingerprinting of genomic DNA by ribotyping and PFGE produced a significant variation in results, depending on the serotype of the strain. Both PstI/SphI ribotyping and XbaI-PFGE provided a similar degree of strain differentiation for serotype Derby and serotype Senftenberg, only marginally lower than that achieved by plasmid profiling. Ribotyping was less sensitive than PFGE when applied to serotype Mbandaka or serotype Montevideo. Serotype Gold Coast isolates were found to be nontypeable by XbaI-PFGE, and a significant proportion of them were found to be plasmid free. A similar situation applies to a number of serotype Livingstone isolates which were nontypeable by plasmid profiling and/or PFGE. In summary, the serotype of the isolates has a considerable influence in deciding the best typing strategy; a single method cannot be relied upon for discriminating between strains, and a combination of typing methods allows further discrimination.

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Characterisation of strains of Salmonella serotype Livingstone by multiple typing

Cited by 19 publications

References 18 publications

Nosocomial Outbreak Caused by Salmonella enterica Serotype Livingstone Producing CTX-M-27 Extended-Spectrum β-Lactamase in a Neonatal Unit in Sousse, Tunisia

Nosocomial Outbreak Caused by Salmonella enterica Serotype Livingstone Producing CTX-M-27 Extended-Spectrum β-Lactamase in a Neonatal Unit in Sousse, Tunisia

Diversity of Strains of Salmonella enterica Serotype Enteritidis from English Poultry Farms Assessed by Multiple Genetic Fingerprinting

Molecular Typing of Salmonella Serotypes Prevalent in Animals in England: Assessment of Methodology

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