Characterisation of Streptococcus thermophilus CNRZ1205 and its cured and re-lysogenised derivatives

Abstract

Streptococcus thermophilus CNRZ1205 is the lysogenic host for the temperate phage ÖO1205. A derivative of CNRZ1205 was isolated which was cured of ÖO1205 and this strain was used to construct a re-lysogenised derivative. Pulse field gel electrophoresis and sequencing of the attachment site regions confirmed that excision and re-integration of the phage was a site-specific event. Interestingly, cells from the cured, as well as its re-lysogenised derivative, were found to have a very long chain length. ß

Identification of four loci isolated from two Streptococcus thermophilus phage genomes responsible for mediating bacteriophage resistance

“…2.1. Bacterial strains, phage and growth media S. thermophilus phages and strains used in this study have been described by Le Marrec et al [4] and Stanley et al [5]. Growth of bacterial strains, phage induction and isolation, and plaque assays and spot assays were performed as described previously [3^5].…”

Identification of four loci isolated from twoStreptococcus thermophilusphage genomes responsible for mediating bacteriophage resistance

“…S. thermophilus phages and strains used in this study have been described by Le Marrec et al [4] and Stanley et al[5]. Growth of bacterial strains, phage induction and isolation, and plaque assays and spot assays were performed as described previously [3–5].…”