Characterisation of target antigen of cell-mediated immunity in Mycobacterium tuberculosis H37RV

Hattikudur, Sandhya; Kamat, R. S.

doi:10.1099/00222615-18-1-17

Cited by 2 publications

(7 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reference antigen could not only elicit a delayed hypersensitivity response in mice immunised with live M . tuberculosis strain H37Rv, it could also generate activated macrophages via T cell interaction (Hattikudur and Kamat, 1984). Because this purified antigen was free of carbohydrates and lipids, the reference antiserum raised against it would react against protein antigens only.…”

Section: Discussionmentioning

confidence: 99%

“…The antigen capable of eliciting effector responses of cell-mediated immunity against M. tuberculosis strain H37Rv was purified as described by Hattikudur and Kamat (1984). The antiserum was raised in rabbits by four injections of 100 pg of reference antigen of M .…”

Section: Methodsmentioning

confidence: 99%

“…Two-dimensional immunoelectrophoresis (2D-IEP), rocket immunoelectrophoresis and agar gel diffusion precipitation techniques were performed as described by Hattikudur and Kamat (1984).…”

Section: Methodsmentioning

confidence: 99%

“…tuberculosis strain H37Rv and subjected to rocket immunoelectrophoresis into a gel containing antiserum against the reference antigen, as described previously (Hattikudur and Kamat, 1984). Precipitin peaks so developed were checked for antigenic identity.…”

Section: Cross-reactivity Of 085-rf Antigens Of Different Mycobacteriamentioning

confidence: 99%

“…In a recent publication (Hattikudur and Kamat, 1984) we reported the isolation and characterisation of a protein antigen of Mycobacterium tuberculosis strain H37Rv that is a target for not only the delayed hypersensitivity response but also for protective cell-mediated immunity against the organism. In this report we present evidence of cross-reactivity between different mycobacteria and M .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Polymorphism of a Mycobacterial Antigen Participating in Cell-Mediated Immunity

Hattikudur

Kamat

1985

Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

SUMMARY. The cross-reactivity of crude sonic extracts of six species of mycobacteria was studied by skin tests in mice and guinea pigs immunised with live Mycobacterium tuberculosis strain H37Rv, and in patients with pulmonary tuberculosis. All slowly growing mycobacteria elicited a strong delayed hypersensitivity response, M . vaccae a moderate response and M . phlei a poor or no response. A specific target antigen for cell-mediated immunity, known to be present in M . tuberculosis, was also present in all the mycobacteria studied. This shared antigen was shown, by immunoprecipitation tests, to be identical in all the slowly growing species but only a partial reaction of identity was obtained with M . phlei. It is concluded that the antigen shared by the slowly growing mycobacteria is immunodominant in cell-mediated immunity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Two-dimensional immunoelectrophoresis (2D-IEP), rocket immunoelectrophoresis and agar gel diffusion precipitation techniques were performed as described by Hattikudur and Kamat (1984).…”

Section: Methodsmentioning

confidence: 99%

Section: Cross-reactivity Of 085-rf Antigens Of Different Mycobacteriamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Polymorphism of a Mycobacterial Antigen Participating in Cell-Mediated Immunity

Hattikudur

Kamat

1985

Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

Murine CD8⁺ T suppressors against mycobacterial 65‐kDa antigen compete for IL‐2 and show lack of major histocompatibility complex‐imposed restriction specificity in antigen recognition

et al. 1993

View full text Add to dashboard Cite

The mechanism of antigen-specific suppression and reasons for aberrant major histocompatibility complex (MHC) class II restriction mediated by CD8+ T cells was investigated in a previously reported murine model of immunosuppression, generated by intraperitoneal priming with Mycobacterium vaccae. Both the CD4+ T helper cells (Th) and CD8+ T suppressor cell (Ts) of M. vaccae-primed mice recognized the 65-kDa antigen of the bacillus, presented by I-A and I-E, respectively. The CD8+ Ts could inhibit non-antigen-specific proliferation of primed CD4+ T cells induced by the exogenously added interleukin (IL)-2 (concanavalin A-stimulated culture supernatant). For inhibition, the Ts had to be activated by the 65-kDa antigen. The degree of inhibition was dependent upon the amount of added IL-2 and the relative numbers of primed CD8+ and CD4+ T cells. On incubation with antigen-presenting cells, and the 65-kDa antigen, the primed CD8+ T cells absorbed IL-2 as efficiently as primed CD4+ T cells. Based on this, it was concluded that the primed CD8+ T cells induced suppression by competition for IL-2. Employing the same model, the MHC restriction of recognition of the suppressor epitope of the 65-kDa antigen by the CD8+ Ts was investigated. The epitopes presented by diverse MHC class II molecules, such as self I-A, I-E and even allogeneic I-E were similar, because they were recognized by the same population of primed CD8+ Ts. Further, immunization of C57BL/6 mice with Ltk-cells expressing H-2 DkKk alloantigens, stimulated CD8+ T cells capable of recognizing M.vaccae 65-kDa antigen. Based on these data, it was proposed that recognition of the suppressor epitope of the 65-kDa antigen by the primed CD8+ Ts exhibits lack of restriction specificity imposed by MHC diversity.

show abstract

Characterisation of target antigen of cell-mediated immunity in Mycobacterium tuberculosis H37RV

Cited by 2 publications

References 5 publications

Polymorphism of a Mycobacterial Antigen Participating in Cell-Mediated Immunity

Polymorphism of a Mycobacterial Antigen Participating in Cell-Mediated Immunity

Murine CD8⁺ T suppressors against mycobacterial 65‐kDa antigen compete for IL‐2 and show lack of major histocompatibility complex‐imposed restriction specificity in antigen recognition

Contact Info

Product

Resources

About

Characterisation of target antigen of cell-mediated immunity in Mycobacterium tuberculosis H37RV

Cited by 2 publications

References 5 publications

Polymorphism of a Mycobacterial Antigen Participating in Cell-Mediated Immunity

Polymorphism of a Mycobacterial Antigen Participating in Cell-Mediated Immunity

Murine CD8+ T suppressors against mycobacterial 65‐kDa antigen compete for IL‐2 and show lack of major histocompatibility complex‐imposed restriction specificity in antigen recognition

Contact Info

Product

Resources

About

Murine CD8⁺ T suppressors against mycobacterial 65‐kDa antigen compete for IL‐2 and show lack of major histocompatibility complex‐imposed restriction specificity in antigen recognition