2003
DOI: 10.1039/b300443k
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Characterisation of the active site of a newly-discovered and potentially significant post-proline cleaving endopeptidase called ZIP using LC-UV-MS

Abstract: Abbreviations AbstractThere are enzymes that specifically recognise the amino acid proline within peptides and proteins that are called post-proline cleaving enzymes. Many of them are implicated in neurodegenerative disorders and psychiatric diseases. ZIP is a newly-discovered one of these peptidases. In this work, it has been purified from bovine serum and subjected to various analytical studies in order to characterise it. A series of reactions between synthesised peptides and ZIP were carried out in order t… Show more

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Cited by 3 publications
(2 citation statements)
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“…Direct infusion MS analysis, which was initially used preferentially ionised some components over others that led to the failure to identify any product formation post incubation. LC-MS analysis using the Bruker/Hewlett-Packard Esquire LC, equipped with an electrospray source (ESI) was then chosen to identify each product of hydrolysis (McMahon et al 2003). This enabled the compounds of interest to only become singly charged and so their molecular weight being readily obtained.…”
Section: Lc-ms Analysismentioning
confidence: 99%
“…Direct infusion MS analysis, which was initially used preferentially ionised some components over others that led to the failure to identify any product formation post incubation. LC-MS analysis using the Bruker/Hewlett-Packard Esquire LC, equipped with an electrospray source (ESI) was then chosen to identify each product of hydrolysis (McMahon et al 2003). This enabled the compounds of interest to only become singly charged and so their molecular weight being readily obtained.…”
Section: Lc-ms Analysismentioning
confidence: 99%
“…Different extracellular proteins with PE activity have been described. In serum, a PE activity, insensitive to specific POP inhibitors, has been identified as fibroblast activation protein or seprase, but important levels of PE activity itself, sensitive to POP‐specific inhibitors, have also been detected [4,5], although confirmation of this enzyme’s identity, by direct sequencing or by antibody binding, has not been provided.…”
mentioning
confidence: 99%