Characterisation of the ATP‐dependent phosphofructokinase gene family from <i>Arabidopsis thaliana</i>

Mustroph, Angelika; Sonnewald, Uwe; Biemelt, Sophia

doi:10.1016/j.febslet.2007.04.060

Cited by 70 publications

(66 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Arabidopsis thaliana, 5 to 10% of the cytosolic isoforms of each glycolytic enzyme is associated with the outer surface of mitochondria. This has been independently corroborated by green fluorescent protein tagging of certain isoforms of Arabidopsis phosphofructokinase (Mustroph et al, 2007). There is also good evidence from a range of plant species that hexokinase associates with the outer mitochondrial membrane (Dry et al, 1983;da-Silva et al, 2001;DamariWeissler et al, 2006;Kim et al, 2006).…”

Section: Introductionmentioning

confidence: 66%

Glycolytic Enzymes Associate Dynamically with Mitochondria in Response to Respiratory Demand and Support Substrate Channeling

et al. 2007

View full text Add to dashboard Cite

In Arabidopsis thaliana, enzymes of glycolysis are present on the surface of mitochondria and free in the cytosol. The functional significance of this dual localization has now been established by demonstrating that the extent of mitochondrial association is dependent on respiration rate in both Arabidopsis cells and potato (Solanum tuberosum) tubers. Thus, inhibition of respiration with KCN led to a proportional decrease in the degree of association, whereas stimulation of respiration by uncoupling, tissue ageing, or overexpression of invertase led to increased mitochondrial association. In all treatments, the total activity of the glycolytic enzymes in the cell was unaltered, indicating that the existing pools of each enzyme repartitioned between the cytosol and the mitochondria. Isotope dilution experiments on isolated mitochondria, using 13C nuclear magnetic resonance spectroscopy to monitor the impact of unlabeled glycolytic intermediates on the production of downstream intermediates derived from 13C-labeled precursors, provided direct evidence for the occurrence of variable levels of substrate channeling. Pull-down experiments suggest that interaction with the outer mitochondrial membrane protein, VDAC, anchors glycolytic enzymes to the mitochondrial surface. It appears that glycolytic enzymes associate dynamically with mitochondria to support respiration and that substrate channeling restricts the use of intermediates by competing metabolic pathways.

show abstract

Section: Introductionmentioning

confidence: 66%

Glycolytic Enzymes Associate Dynamically with Mitochondria in Response to Respiratory Demand and Support Substrate Channeling

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The fact that the PPi-PFK of both monocot and dicot is similar to ATREPX1 and that all these sequences form a monophyletic clade could lead us to infer that domestication of ATREPX1 by the gene occurred before the diversiWcation of the Xowering plants. Since the alignment of the PPi-PFK protein of Arabidopsis thaliana against the translated ATREPX1 sequences showed two stop codons in the element that are absent in the host protein, and taking into consideration that the protein of Arabidopsis thaliana is functional (Mustroph et al 2007) and that exon 9 encodes an active site conserved residue (Moore et al 2002), it is more likely that ATREPX1 has captured the two exons of PPi-PFK and not the opposite. However, we cannot rule out the possibility that the mutations within ATREPX1 occurred after domestication.…”

Section: Transposable Elements-mediated Transduction Of Host Gene Framentioning

confidence: 99%

Transposable elements in Coffea (Gentianales: Rubiacea) transcripts and their role in the origin of protein diversity in flowering plants

et al. 2008

View full text Add to dashboard Cite

Transposable elements are major components of plant genomes and they influence their evolution, acting as recombination hot spots, acquiring specific cell functions or becoming part of protein-coding regions. The latter is the subject of the present analysis. This study is a report on the annotation of transposable elements (TEs) in expressed sequences of Coffea arabica, Coffea canephora and Coffea racemosa, showing the occurrence of 383 ESTs and 142 unigenes with TE fragments in these three Coffea species. Based on selected unigenes, it was possible to suggest 26 putative proteins with TE-cassette insertions, demonstrating a likely contribution to protein variability. The genes for two of those proteins, the fertility restorer (FR) and the pyrophosphate-dependent phosphofructokinase (PPi-PFKs) genes, were selected for evaluating the impact of TE-cassettes on host gene evolution of other plant genomes (Arabidopsis thaliana, Oryza sativa and Populus trichocarpa). This survey allowed identifying a FR gene in O. sativa harboring multiple insertions of LTR retrotransposons that originated new exons, which however does not necessarily mean a case of molecular domestication. A possible transduction event of a fragment of the PPi-PFK beta-subunit gene mediated by Helitron ATREPX1 in Arabidopsis thaliana was also highlighted.

show abstract

“…In metabolic pathways concerned with sucrose or monosaccharide 1-phosphate, PPi is produced or hydrolyzed by reversible reactions catalyzed by UDP-glucose pyrophosphorylase (Park et al, 2010), UDP-sugar pyrophosphatase (Kotake et al, 2007), and PPi-dependent phosphofructokinase (Mustroph et al, 2007). Changes in PPi concentration are known to affect these reactions in a specific direction.…”

Section: Marked Accumulation Of H + -Ppase In Specific Tissuesmentioning

confidence: 99%

Dynamics of Vacuoles and H+-Pyrophosphatase Visualized by Monomeric Green Fluorescent Protein inArabidopsis: Artifactual Bulbs and Native Intravacuolar Spherical Structures

et al. 2014

View full text Add to dashboard Cite

We prepared Arabidopsis thaliana lines expressing a functional green fluorescent protein (GFP)-linked vacuolar H + -pyrophosphatase (H + -PPase) under the control of its own promoter to investigate morphological dynamics of vacuoles and tissue-specific expression of H + -PPase. The lines obtained had spherical structures in vacuoles with strong fluorescence, which are referred to as bulbs. Quantitative analyses revealed that the occurrence of the bulbs correlated with the amount of GFP. Next, we prepared a construct of H + -PPase linked with a nondimerizing GFP (mGFP); we detected no bulbs. These results indicate that the membranes adhere face-to-face by antiparallel dimerization of GFP, resulting in the formation of bulbs. In plants expressing H + -PPase-mGFP, intravacuolar spherical structures with double membranes, which differed from bulbs in fluorescence intensity and intermembrane spacing, were still observed in peripheral endosperm, pistil epidermis and hypocotyls. Four-dimensional imaging revealed the dynamics of formation, transformation, and disappearance of intravacuolar spherical structures and transvacuolar strands in living cells. Visualization of H + -PPase-mGFP revealed intensive accumulation of the enzyme, not only in dividing and elongating cells but also in mesophyll, phloem, and nectary cells, which may have high sugar content. Dynamic morphological changes including transformation of vacuolar structures between transvacuolar strands, intravacuolar sheet-like structures, and intravacuolar spherical structures were also revealed.

show abstract

Characterisation of the ATP‐dependent phosphofructokinase gene family from Arabidopsis thaliana

Cited by 70 publications

References 65 publications

Glycolytic Enzymes Associate Dynamically with Mitochondria in Response to Respiratory Demand and Support Substrate Channeling

Glycolytic Enzymes Associate Dynamically with Mitochondria in Response to Respiratory Demand and Support Substrate Channeling

Transposable elements in Coffea (Gentianales: Rubiacea) transcripts and their role in the origin of protein diversity in flowering plants

Dynamics of Vacuoles and H+-Pyrophosphatase Visualized by Monomeric Green Fluorescent Protein inArabidopsis: Artifactual Bulbs and Native Intravacuolar Spherical Structures

Contact Info

Product

Resources

About