Characterisation of the Bioremediation of Chromate by Methylococcus capsulatus

Enbaia, Salaheldeen S.

doi:10.7190/shu-thesis-00200

Cited by 1 publication

(2 citation statements)

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“…Unlike Methylobacter Type I, the relative abundance of Methylcoccus was higher in EPP + 13 C + M1C2, EPP + 13 C + M2C2, EPP + 13 C + PE, EPP + 13 C + M1C2 + PE, and EPP + 13 C + M2C2 + PE by 8.99%, 7.33%, 1.63%, 11.23%, and 12.37, respectively. Methylococcus have been widely used as single and/or mix cultures for bioremediation of HMs such as lead and chromium [ 14 , 34 ]. The presence of HMs and PE stimulated Methylococcus , Methylocystis , and Methylosinus .…”

Section: Resultsmentioning

confidence: 99%

“…In our previous study, an epipelon biofilm was proved to be time- and cost-efficient for HMs removal under elevated methane concentrations [ 14 ]. A single culture of Methylococcus capsulatus (Bath) efficiently bioremediated chromium (Cr 6+ ) over a wide range of concentrations (1.4−1000 mg L −1 ) [ 34 ]. However, to the best of our knowledge, few research articles are available on the bioremediation of Pb 2+ and Cd 2+ by periphytic methanotrophs.…”

Section: Introductionmentioning

confidence: 99%

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Multifunctional Periphytic Biofilms: Polyethylene Degradation and Cd2+ and Pb2+ Bioremediation under High Methane Scenario

Faheem

Shabbir

Zhao

et al. 2020

IJMS

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Priority pollutants such as polyethylene (PE) microplastic, lead (Pb2+), and cadmium (Cd2+) have attracted the interest of environmentalists due to their ubiquitous nature and toxicity to all forms of life. In this study, periphytic biofilms (epiphyton and epixylon) were used to bioremediate heavy metals (HMs) and to biodegrade PE under high (120,000 ppm) methane (CH4) doses. Both periphytic biofilms were actively involved in methane oxidation, HMs accumulation and PE degradation. Epiphyton and epixylon both completely removed Pb2+ and Cd2+ at concentrations of 2 mg L−1 and 50 mg L−1, respectively, but only partially removed these HMs at a relatively higher concentration (100 mg L−1). Treatment containing 12% 13CH4 proved to be most effective for biodegradation of PE. A synergistic effect of HMs and PE drastically changed microbial biota and methanotrophic communities. High-throughput 16S rRNA gene sequencing revealed that Cyanobacteria was the most abundant class, followed by Gammaproteobacteria and Alphaproteobacteria in all high-methane-dose treatments. DNA stable-isotope probing was used to label 13C in a methanotrophic community. A biomarker for methane-oxidizing bacteria, pmoA gene sequence of a 13C-labeled fraction, revealed that Methylobacter was most abundant in all high-methane-dose treatments compared to near atmospheric methane (NAM) treatment, followed by Methylococcus. Methylomonas, Methylocystis, Methylosinus, and Methylocella were also found to be increased by high doses of methane compared to NAM treatment. Overall, Cd+2 had a more determinantal effect on methanotrophic activity than Pb2+. Epiphyton proved to be more effective than epixylon in HMs removal and PE biodegradation. The findings proved that both epiphyton and epixylon can be used to bioremediate HMs and biodegrade PE as an efficient ecofriendly technique under high methane concentrations.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%