Characterisation of the hepatic progenitor cell compartment in normal liver and in hepatitis: An immunohistochemical comparison between dog and man

IJzer, Jooske; Schotanus, Baukje A; Borght, Sara Vander; Roskams, Tania; Kisjes, Ronald; Penning, Louis C.; Rothuizen, J.; Ingh, T.S.G.A.M. van den

doi:10.1016/j.tvjl.2009.02.016

Cited by 33 publications

(47 citation statements)

References 25 publications

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“…5, 6). 6,15,19 The absence of any intermediate cells in the portal and septal DR in these cases might indicate that progenitor cell maturation is restricted by this setting. 12,13 In the normal feline liver, staining for mature Hepar1 was restricted to mature hepatocytes (Fig.…”

Section: Discussionmentioning

confidence: 91%

The Progenitor Cell Compartment in the Feline Liver: An (Immuno)Histochemical Investigation

et al. 2009

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Abstract. The hepatic progenitor compartment is of vital importance in liver regeneration when hepatocellular replication is impaired, as it occurs in acute fulminant hepatitis or severe liver fibrosis. It consists of resident progenitor cells in the normal liver, and ductular reaction and intermediate hepatobiliary cells in diseased livers. An histologic and immunohistochemical study was conducted to demonstrate putative hepatic progenitor cells in the normal liver (n 5 5) and in a range of hepatic diseases (n 5 13) in the cat. Formalin-fixed, paraffin-embedded specimens were stained with HE, the van Gieson stain, and the reticulin stain according to Gordon and Sweet, and immunohistochemically stained for cytokeratin-7 (CK7), human hepatocyte marker 1 (Hepar1), and multidrug resistancebinding protein-2/ATP binding cassette C2 (MRP2). The normal feline liver contains a liver progenitor cell morphologically similar to humans and dogs, which resides in the canal of Hering. In acute and chronic feline liver diseases a ductular reaction is present, whether in the parenchyma or in a portal or septal location. The putative progenitor cells could easily be demonstrated by staining for CK7, whereas they were generally negative for Hepar1 and MRP2. In a parenchymal ductular reaction mitotic figures and cells with an intermediate hepatobiliary phenotype could be demonstrated. This is the first account of hepatic progenitor cells in feline liver.

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Section: Discussionmentioning

confidence: 91%

The Progenitor Cell Compartment in the Feline Liver: An (Immuno)Histochemical Investigation

et al. 2009

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“…The CoH is a channel composed of a partial lining of cholangiocytes, the other half being lined by hepatocytes (Roskams et al 2004;Saxena et al 2004;Roskams 2006;Carpino et al 2012). The HPCs are confirmed to be resident at the bile ductules and/or CoH in human beings (Lowes et al 1999;Roskams et al 2004;Saxena et al 2004), cats (Ijzer et al 2009), dogs (Ijzer et al 2010) and animal models (Vessey and de la Hall 2001). It is strongly suggested that these HPCs can differentiate into both hepatocytic and cholangiocytic cell lineage (Lowes et al 1999;Theise et al 1999;Vessey and de la Hall 2001;Roskams 2006;Stalker and Hayes 2007;Nakanuma et al 2010).…”

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confidence: 97%

Cholangiocarcinoma of possibly hepatic progenitor cell origin in a cow

Ohfuji¹

2012

Veterinary Quarterly

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“…The bulk of ECM in the fibrotic liver is produced by myofibroblast (MF)-like cells. Three different MF-like cells have been described in rat and man based on location and immunohistochemical profile (Cassiman et al 2002;IJzer et al 2010). These three types comprise portal or septal MF, interface MF and perisinusoidally located hepatic stellate cells (HSCs).…”

Section: Liver Fibrosismentioning

confidence: 99%

“…A dog model with an inducible, controllable and reproducible form of chronic hepatitis (CH) with a well understood cause might be a valuable tool for evaluating new therapeutic strategies. Spee et al, IJzer et al and Schotanus et al demonstrated that the molecular pathophysiology of canine fibrotic liver diseases is highly comparable to the pathophysiology of their human counterparts (Spee et al 2006a;Schotanus et al 2009;IJzer et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Copper-induced hepatitis: the COMMD1 deficient dog as a translational animal model for human chronic hepatitis

Favier

Spee

Penning

et al. 2011

Veterinary Quarterly

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Chronic inflammatory liver disease regardless of aetiology leads to failing regeneration and fibrosis, ending in cirrhosis. Both in man and in animals this worldwide health problem has no definitive cure. Chronic liver injury causes hepatic stellate cells to proliferate and differentiate into matrix-producing cells. New therapeutic options will be developed upon detailed understanding of the molecular mechanisms driving liver fibrosis. This may lead to new anti-fibrotic therapies which need to be tested in suitable models before application in the veterinary and human clinic. On the other side, to restore the failing regenerative capacity of the diseased liver cells, adult progenitor cells are of interest, as an alternative to whole organ transplantation. In order to find the most suitable large animal model it is important to recognise that the typical histopathological reaction pattern of the liver can differ between mammalian species. It is therefore imperative that specialists in veterinary internal medicine and pathology, being familiar with the diseases and pathologies of the liver in different animal species, are teaming-up in finding the best models for veterinary and human liver diseases. Several large animal models have been mentioned, like pigs, sheep, and dogs. Based on the observations that man and dog share the same hepatopathies and have identical clinical, pathological and pathogenetic reaction patterns during the development of liver disease, the dog seems to be a properly suited species to test new therapeutic strategies for pets and their best friends.

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Characterisation of the hepatic progenitor cell compartment in normal liver and in hepatitis: An immunohistochemical comparison between dog and man

Cited by 33 publications

References 25 publications

The Progenitor Cell Compartment in the Feline Liver: An (Immuno)Histochemical Investigation

The Progenitor Cell Compartment in the Feline Liver: An (Immuno)Histochemical Investigation

Cholangiocarcinoma of possibly hepatic progenitor cell origin in a cow

Copper-induced hepatitis: the COMMD1 deficient dog as a translational animal model for human chronic hepatitis

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