Characterisation of the RNA Virome of Nine Ochlerotatus Species in Finland

Abstract

RNA viromes of nine commonly encountered Ochlerotatus mosquito species collected around Finland in 2015 and 2017 were studied using next-generation sequencing. Mosquito homogenates were sequenced from 91 pools comprising 16–60 morphologically identified adult females of Oc. cantans, Oc. caspius, Oc. communis, Oc. diantaeus, Oc. excrucians, Oc. hexodontus, Oc. intrudens, Oc. pullatus and Oc. punctor/punctodes. In total 514 viral RdRp polymerase sequences of 159 virus species were recovered, belonging to 25 families or equivalent rank, as follows: Aliusviridae, Aspiviridae, Botybirnavirus, Chrysoviridae, Chuviridae, Endornaviridae, Flaviviridae, Iflaviridae, Negevirus, Partitiviridae, Permutotetraviridae, Phasmaviridae, Phenuiviridae, Picornaviridae, Qinviridae, Quenyavirus, Rhabdoviridae, Sedoreoviridae, Solemoviridae, Spinareoviridae, Togaviridae, Totiviridae, Virgaviridae, Xinmoviridae and Yueviridae. Of these, 147 are tentatively novel viruses. One sequence of Sindbis virus, which causes Pogosta disease in humans, was detected from Oc. communis from Pohjois-Karjala. This study greatly increases the number of mosquito-associated viruses known from Finland and presents the northern-most mosquito-associated viruses in Europe to date.

Metatranscriptomic Sequencing of Medically Important Mosquitoes Reveals Extensive Diversity of RNA Viruses and Other Microbial Communities in Western Australia

“…We report two members of this family, Wilkie qin-like virus 1 (WQLV1) and Fitzroy Crossing qinvirus 1 (FCQV1), both clustering with viruses previously sequenced from mosquitoes ( Figure S11 ). Although the association of WQLV1 with mosquito hosts has been questioned [ 11 ], two recent studies have sequenced related viruses from mosquitoes sampled elsewhere [ 32 , 43 ], thus strengthening support for association with mosquito hosts.…”

Enhanced Viral Metagenomics with Lazypipe 2

“…Here, we presented Lazypipe 2, an updated version of our mNGS pipeline with significant improvements in code ability, transparency and support for new software components. The previous version, Lazypipe, and now Lazypipe 2 have been used, contributing to virus discovery and demonstrating its potential for unbiased NGS-based studies [ 23 , 24 , 25 , 26 , 27 ].…”

“…Our research group has contributed to this development with the Lazypipe mNGS pipeline designed primarily for virus discovery from clinical, animal and environmental samples with minimal requirements in terms of bioinformatic expertise and/or resources [ 22 ]. Lazypipe has been adopted as a standard module for mNGS analysis at the Finnish IT Center for Science ( , accessed on 21 December 2022) and has been successfully applied to detect and characterize a multitude of novel viral pathogens from a variety of different sample types, including arthropod vectors [ 23 , 24 ], mosquitos [ 25 ], farm animals [ 26 ] and wildlife [ 27 ]. Here, we present an enhanced version of our mNGS pipeline, Lazypipe 2.…”

Highly divergent and diverse viral community infecting sylvatic mosquitoes from Northeast Brazil

“…albopictus and Ae. aegypti (He et al, 2021), Psorophora species (Charles et al, 2018), Ochlerotatus (Truong Nguyen et al, 2022) and Armigeres (Thongsripong et al, 2021). Here we analyzed the virome of three Aedini species, our results showed a low diversity of viral families within pools analyzed ( Figure 2 ).…”