Characterisation of Trichomonas vaginalis Isolates Collected from Patients in Vienna between 2019 and 2021

Lesiak-Markowicz, Iwona; Walochnik, Julia; Stary, Angelika; Fürnkranz, Ursula

doi:10.3390/ijms232012422

Cited by 3 publications

(1 citation statement)

References 62 publications

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“…After obtaining axenic cultures, susceptibility analysis to MET was performed [43]. MET susceptibility testing was performed in 96-well flat-bottomed microtiter plates in 5% CO 2 at 37 • C. Two-fold serial dilutions of MET were performed in TYM medium.…”

Section: Pharmacological Activity Of Met-crg-cnw-cgmentioning

confidence: 99%

Carrageenan/Chitin Nanowhiskers Cryogels for Vaginal Delivery of Metronidazole

et al. 2023

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The development of polymeric carriers based on partially deacetylated chitin nanowhiskers (CNWs) and anionic sulfated polysaccharides is an attractive strategy for improved vaginal delivery with modified drug release profiles. This study focuses on the development of metronidazole (MET)-containing cryogels based on carrageenan (CRG) and CNWs. The desired cryogels were obtained by electrostatic interactions between the amino groups of CNWs and the sulfate groups of CRG and by the formation of additional hydrogen bonds, as well as by entanglement of carrageenan macrochains. It was shown that the introduction of 5% CNWs significantly increased the strength of the initial hydrogel and ensured the formation of a homogeneous cryogel structure, resulting in sustained MET release within 24 h. At the same time, when the CNW content was increased to 10%, the system collapsed with the formation of discrete cryogels, demonstrating MET release within 12 h. The mechanism of prolonged drug release was mediated by polymer swelling and chain relaxation in the polymer matrix and correlated well with the Korsmeyer–Peppas and Peppas–Sahlin models. In vitro tests showed that the developed cryogels had a prolonged (24 h) antiprotozoal effect against Trichomonas, including MET-resistant strains. Thus, the new cryogels with MET may be promising dosage forms for the treatment of vaginal infections.

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Section: Pharmacological Activity Of Met-crg-cnw-cgmentioning

confidence: 99%

Carrageenan/Chitin Nanowhiskers Cryogels for Vaginal Delivery of Metronidazole

et al. 2023

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Mucoadhesive polyelectrolyte complexes of fucoidan and chitin nanowhiskers to prolong the antiprotozoal activity of metronidazole

Dubashynskaya,

Petrova,

Ustyukhina

et al. 2025

Carbohydrate Polymers

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The Prevalence of Genital Mycoplasmas and Coinfection with Trichomonas vaginalis in Female Patients in Vienna, Austria

et al. 2023

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Trichomonas vaginalis causes trichomoniasis, the most recurrent sexually transmitted infection (STI) worldwide. Genital mycoplasmas, not considered STI agents, are frequently isolated from the female genital tract. A symbiosis between Mycoplasma species and T. vaginalis has been described. The aim of this study was to conduct molecular-based analyses of vaginal specimens, thus assessing the prevalence of non-STI Mycoplasma infections. In total, 582 samples from female patients and an additional 20 T. vaginalis isolates were analyzed by PCR using Mycoplasma specific 16S rRNA primers, and the obtained PCR products were sequenced. Mycoplasma species were detected in 28.2% of the collected vaginal samples. Mycoplasma hominis was found in 21.5% of the specimens, Ureaplasma species were found in 7.5% of the samples. The molecular data of the newly described species, CandidatusMycoplasma girerdii, were obtained for the first time in Austria, in a sample also positive for T. vaginalis. Analyses of the cultivated T. vaginalis strains confirmed the presence of M. hominis in two out of 20 samples. A comparably high prevalence of genital mycoplasmas was revealed through advanced diagnostic assays, with M. hominis and U. parvum being the most prevalent species. The previously described symbiotic relationship between M. hominis and T. vaginalis was confirmed.

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Characterisation of Trichomonas vaginalis Isolates Collected from Patients in Vienna between 2019 and 2021

Cited by 3 publications

References 62 publications

Carrageenan/Chitin Nanowhiskers Cryogels for Vaginal Delivery of Metronidazole

Carrageenan/Chitin Nanowhiskers Cryogels for Vaginal Delivery of Metronidazole

Mucoadhesive polyelectrolyte complexes of fucoidan and chitin nanowhiskers to prolong the antiprotozoal activity of metronidazole

The Prevalence of Genital Mycoplasmas and Coinfection with Trichomonas vaginalis in Female Patients in Vienna, Austria

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