Characterisation of water-extractable soil organic phosphorus by phosphatase hydrolysis

Phosphatase activity in temperate pasture soils: Potential regulation of labile organic phosphorus turnover by phosphodiesterase activity

“…This hypothesis is supported by evidence that RNA was hydrolysed much more slowly than simple phosphate monoesters (glycerophosphate and 3V mononucleotides of RNA) in a temperate sandy loam under grass in central USA (pH 6.5, total C 0.9%) (Bowman and Cole, 1978). Further, large proportions of the water-extractable organic P in some Australian pasture soils occurred as phosphate diesters, with labile monoesters present in negligible amounts (Turner et al, 2002b). The differences in the influence of soil pH on the two phosphatases may be due in part to the dominant microbes present in acidic and neutral soils (Paul and Clark, 1996).…”

Sampling, sample treatment and quality assurance issues for the determination of phosphorus species in natural waters and soils

“…Indeed, functional classification of water-extractable organic phosphorus from dry Australian pasture soils revealed similar proportions of microbially derived phosphate diesters and phytic acid from the non-biomass soil organic matter [59]. A similar mechanism probably occurs following freezing and thawing [60].…”

“…The recovery of alkaline phosphatase hydrolysable phosphorus was low in natural waters but good in sediments [146]. They also applied alkaline phosphatase to soils [59]. Acid and alkaline phosphatase and phytase have been used in combination to investigate organic phosphorus speciation in soils [149].…”

“…wheat, first acts on the C 6 atom while that from microbial sources acts on the C 3 atom. McKelvie et al [59,152] used a FI system with immobilised phytase for the determination of phytic acid in soils. Adenosine-5 -triphosphate was also hydrolysed but in low yields compared with phytic acid.…”

Quantification of pyrophosphate in soil solution by pyrophosphatase hydrolysis

“…Shand and Smith, 1997;Hayes et al, 2000;Turner et al, 2002). Phosphatases added to soil solution or extracts catalyze the hydrolysis of specific functional organic phosphorus groups, allowing detection of the released orthophosphate by routine colorimetry.…”