Characterisation of yeast microbial fuel cell with the yeast Arxula adeninivorans as the biocatalyst

Haslett, Nicholas D.; Rawson, Frankie J.; Barrière, Frédéric; Kunze, Gotthard; Pasco, Neil; Gooneratne, Ravi; Baronian, Keith

doi:10.1016/j.bios.2011.02.011

Cited by 84 publications

(23 citation statements)

References 19 publications

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“…In general, yeast based MFCs perform better than cyanobacteria but still have a lower power output than bacterial fuel cells 25 . An experimentally observed current of 16.09 ± 8 μA was recorded when the cell was operating under its lowest impedance in a previous S .cerevisiae based MFC 15 .…”

Section: Resultsmentioning

confidence: 96%

Exploration and comparison of inborn capacity of aerobic and anaerobic metabolisms ofSaccharomyces cerevisiaefor microbial electrical current production

Mao¹,

Verwoerd²

2013

Bioengineered

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Saccharomyces cerevisiae possesses numerous advantageous biological features, such as being robust, easily handled, mostly non-pathogenic and having high catabolic rates, etc., which can be considered as merits for being used as a promising biocatalyst in microbial fuel cells (MFCs) for electricity generation. Previous studies have developed efficient MFC configurations to convert metabolic electron shuttles, such as cytoplasmic NADH, into usable electric current. However, no studies have elucidated the maximum potential of S. cerevisiae for current output and the underlying metabolic pathways, resulting from the interaction of thousands of reactions inside the cell during MFC operation. To address these two key issues, this study used in silico metabolic engineering techniques, flux balance analysis (FBA), and flux variability analysis with target flux minimization (FATMIN), to model the metabolic perturbation of S. cerevisiae under the MFC-energy extraction. The FBA results showed that, in the cytoplasmic NADH-dependent mediated electron transfer (MET) mode, S. cerevisiae had a potential to produce currents at up to 5.781 A/gDW for the anaerobic and 6.193 A/gDW for the aerobic environments. The FATMIN results showed that the aerobic and anaerobic metabolisms are resilient, relying on six and five contributing reactions respectively for high current production. Two reactions, catalyzed by glutamate dehydrogenase (NAD) (EC 1.4.1.3) and methylene tetrahydrofolate dehydrogenase (NAD) (EC 1.5.1.5), were shared in both current-production modes and contributed to over 80% of the identified maximum current outputs. It is also shown that the NADH regeneration was much less energy costly than biomass production rate. Taken together, our finding suggests that S. cerevisiae should receive more research effort for MFC electricity production.

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Section: Resultsmentioning

confidence: 96%

Exploration and comparison of inborn capacity of aerobic and anaerobic metabolisms ofSaccharomyces cerevisiaefor microbial electrical current production

Mao¹,

Verwoerd²

2013

Bioengineered

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“…The biocatalytic activity of the nonconventional yeast A. adeninvorans in a mediator-less dual chamber MFC was investigated [58]. Results showed that A. adeninvorans was effectively used as a biocatalyst ion in the MFC, generating a power of more than 0.025 W/m 2 .…”

Section: A Adeninvoransmentioning

confidence: 97%

“…Several yeast strains have been studied as biocatalysts in MFC with or without external mediator such as Saccharomyces cerevisiae (S. cerevisiae) [41][42][43][44][45][46][47][48][49][50][51][52], Candida melibiosica 2491 (C. melibiosica) [53][54][55][56], Hansenula anomala (H. anomala) [40], Hansenula polymorpha (Hansenula polymorpha) [57], Arxula adeninivorans (A. adeninivorans) [58] and Kluyveromyces marxianus (K. marxianus) [59].…”

Section: Yeast As a Biocatalyst In Mfcsmentioning

confidence: 99%

Yeast as a Biocatalyst in Microbial Fuel Cell

Sayed¹,

Abdelkareem²

2017

Old Yeasts - New Questions

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“…Although some yeasts, such as Saccharomyces cerevisiae [53,54], Arxula adeninivorans [55] and Candida melibiosica [56,57], have been employed as catalysts in MFC, electricity-generating bacteria, such as Actinobacillus succinogenes [58], Clostridium species [59,60], Escherichia coli [61][62][63], Geobacter species [64][65][66], Klebsiella pneumoniae [67,68], Pseudomonas species [69,70], and Shewanella species [71][72][73], are commonly used.…”

Section: Microorganism Substrate Fuels and Operation Conditionsmentioning

confidence: 99%

Low-temperature microbial and direct conversion of lignocellulosic biomass to electricity: Advances and challenges

Zhao

Liu

Deng

et al. 2017

Renewable and Sustainable Energy Reviews

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Conversion of lignocellulosic biomass to electricity using fuel cell technologies is a promising but challenging research topic for sustainable electricity production. This is because that lignocellulosic biomass generally cannot be directly used as a fuel for electricity generation in a conventional fuel cell with high efficiency. Typical fuel cells that can convert lignocellulosic biomass to electricity under mild conditions (< 100 o C) include microbial fuel cells (MFC) and novel direct biomass fuel cells (DBFC) such as that mediated by polyoxometalates (POMs) developed recently. However, the efficiency and power output for these low-temperature fuel cells still need to be improved for practical applications. In this review, we focus on the research advances of electricity generation in fuel cells that can be operated at low temperatures. More specifically, we discussed the progress, challenge and perspectives of biomass-fueled MFCs. Recent interesting researches on DBFC were also highlighted in terms of the efficiency, principles, and technological obstacles. As concluded in this work, lignocellulosic biomass is a promising feedstock for fuel cells because it is renewable, carbon neutral, and sustainable. However, the power density of lignocelluose-fueled MFC are usually far below that required for commercial applications.Improving fermentable sugar release from lignocellulosic biomass and increasing the cell  Corresponding authors: jzhu@fs.fed.us; yulin.deng@ipst.gatech.edu 2 output power are the main research points. DBFC can obtain a high theoretical exergy recovery; however, it is still in its early stage of development with low efficiency. More research should be focused on the electrode development, cell design, parameter optimization, process integration, as well as understanding fundamental process mechanisms.

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Characterisation of yeast microbial fuel cell with the yeast Arxula adeninivorans as the biocatalyst

Cited by 84 publications

References 19 publications

Exploration and comparison of inborn capacity of aerobic and anaerobic metabolisms ofSaccharomyces cerevisiaefor microbial electrical current production

Exploration and comparison of inborn capacity of aerobic and anaerobic metabolisms ofSaccharomyces cerevisiaefor microbial electrical current production

Yeast as a Biocatalyst in Microbial Fuel Cell

Low-temperature microbial and direct conversion of lignocellulosic biomass to electricity: Advances and challenges

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